Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 628 results for: rel

Showing: 81 - 100 of 628 results

  1. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...the reaction on an agarose gel to determine the relative sizes of the resulting DNA fragments. Restriction...) shows 3 possible plasmid conformations, with relaxed and nicked marked with asterisks (*). When the ...conformations the most abundant being: supercoiled, relaxed and nicked. If your digest lanes look like your...

  3. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    .... A unique evolutionary feature of K. phaffii, relative to S. cerevisiae, is that it predominantly uses...enabling study of human-relevant pathways (Liu et al., 2017). Beyond human relevance, yeast have many applications...following decades, Phillips made the decision to release the K. phaffii expression system to the general...glucose or glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily it...

  4. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays due to only having...antibody per target just isn’t enough and we recently released Anti-GFP [N86/8R] as another option for targeting...tested hfYFP in expansion microscopy as well as correlative light and electron microscopy and observed high...

  7. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...adding more terms.  Weighting We determined the relative importance of different bits of information for...completeness of our information, and the accuracy and relevance of our search results. The footer and improved...search engine to return only the filters that are relevant to a specific query and category. For example,...shortcuts to specific catalog items as well as related items (for example, a kit that contains a plasmid...

  8. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al., 2022).    ...Antidrug Antibody Formation in Oncology: Clinical Relevance and Challenges. The oncologist, 21(10), 1260–1268...

  9. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ... a given disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based SDM has lagged...directed mutagenesis methods Current SDM methods rely on in vitro assembly. To make a point mutation, ...some of which are proprietary. These methods also rely on ssDNA ends to match up regions of homology, so...better one. Trehan et al. wondered if, instead of relying on in vitro assembly, they could make the E. coli...

  10. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...significantly improve the reproducibility and reliability of science publications. Peer reviewing To make...make sure your manuscript is perceived as a reliable source of information, it is best to submit it to...

  11. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ...populations and to study relative protein expression. Its reliability will be entirely dependent on the selectivity...and the abundance of the epitope in the sample, relative to cross-reactive antigens – that is, an antibody... are relying on correlations between actual antibody labeling and expected labeling. Correlation cannot...practice to combine correlation with other approaches when possible. Finally, correlation of -omics data ... antibody selectivity even more challenging. Correlation with RNA or Proteomic data from multiple cell...identification of the cell types of interest. By correlating your data sets, you can increase confidence that... of an orthogonal approach to understand the relationship between antibody staining intensity by flow ...

  12. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...raw %}{"no_follow":false,"open_in_new_tab":true,"rel":"noopener","sponsored":false,"url":{"content_id"...critical roles in nerve and muscle function, hormone release, and cell signaling throughout the body. Because...fluorescent toolbox Modern neuroscience experiments often rely on multiplexing, where multiple fluorescent tools...

  13. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...induce a lower inflammatory response within hosts. Relative to the other viruses discussed in this article...lentivirus and gamma-retrovirus are so closely related, their required components are very similar. While...

  14. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated...used to achieve transient, high expression of the relevant cargo without the fear of random genomic integration...

  15. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...their work on the shoulders of giants. The still relatively young movement of sharing open hardware has a...device for individual experiments. Manufacturers rarely provide the construction plans or CAD (computer...enabling this work. Sharing open-source plans that rely on such tools has allowed the community to regularly...bringing cutting-edge research to everybody by relying on tailored image processing and low-cost optical...

  17. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...identifies proteins solely by weight and size, relying on the signaling molecule solely asmeans of visualization...assays.  A brief note Since the indirect method relies on multiple secondary antibodies attaching to a...

  19. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is... Links to relevant resources The homepage displays a graphic describing CasIDs and relevant properties... What are the features? CasPEDIA has collected relevant information on all the Cas enzymes you know and...

  20. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...efficiency of Cas9 cleavage is relatively high while the efficiency of HDR is relatively low, meaning that most...Anzalone et al., 2019). PE3, as originally introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering mismatch...act first. PE4 and PE5, as originally introduced, rely on the PE2 enzyme. PEmax: Optimizing prime editor...
Showing: 81 - 100 of 628 results