.... A unique evolutionary feature of K. phaffii, relative to S. cerevisiae, is that it predominantly uses...enabling study of human-relevant pathways (Liu et al., 2017). Beyond human relevance, yeast have many applications...following decades, Phillips made the decision to release the K. phaffii expression system to the general...glucose or glycerol). K. phaffii is only distantly related to S. pombe and S. cerevisiae; evolutionarily it...

...epitopes on GFP Monoclonal antibodies can yield relatively low signal in immunoassays due to only having...antibody per target just isn’t enough and we recently released Anti-GFP [N86/8R] as another option for targeting...tested hfYFP in expansion microscopy as well as correlative light and electron microscopy and observed high...

... the vaccine, we've been able to measure their relative ability to neutralize.”   Figure 2: Sera...resources on the Addgene blog Browse all COVID-19 related blog posts Learn more about pseudotyping Resources...

...trained on vast amounts of data to calculate the relative immunogenicity score (Mattei et.al., 2022).    ...Antidrug Antibody Formation in Oncology: Clinical Relevance and Challenges. The oncologist, 21(10), 1260–1268...

...make your own. In my next post, I’ll explain the relatively straightforward process of generating your own...

... a given disease. CRISPR has made genomic SDM relatively straightforward, but plasmid-based SDM has lagged...directed mutagenesis methods Current SDM methods rely on in vitro assembly. To make a point mutation, ...some of which are proprietary. These methods also rely on ssDNA ends to match up regions of homology, so...better one. Trehan et al. wondered if, instead of relying on in vitro assembly, they could make the E. coli...

...data behind the figures and results. This is a relatively new development, and it allows reviewers and ...significantly improve the reproducibility and reliability of science publications. Peer reviewing To make...make sure your manuscript is perceived as a reliable source of information, it is best to submit it to...

...populations and to study relative protein expression. Its reliability will be entirely dependent on the selectivity...and the abundance of the epitope in the sample, relative to cross-reactive antigens – that is, an antibody... are relying on correlations between actual antibody labeling and expected labeling. Correlation cannot...practice to combine correlation with other approaches when possible. Finally, correlation of -omics data ... antibody selectivity even more challenging. Correlation with RNA or Proteomic data from multiple cell...identification of the cell types of interest. By correlating your data sets, you can increase confidence that... of an orthogonal approach to understand the relationship between antibody staining intensity by flow ...

...induce a lower inflammatory response within hosts. Relative to the other viruses discussed in this article...lentivirus and gamma-retrovirus are so closely related, their required components are very similar. While...

...mutagenesis in the host. Adenoviruses can deliver relatively large cargo inserts (>8 kb). Adeno-associated...used to achieve transient, high expression of the relevant cargo without the fear of random genomic integration...

...their work on the shoulders of giants. The still relatively young movement of sharing open hardware has a...device for individual experiments. Manufacturers rarely provide the construction plans or CAD (computer...enabling this work. Sharing open-source plans that rely on such tools has allowed the community to regularly...bringing cutting-edge research to everybody by relying on tailored image processing and low-cost optical...

...can be very helpful if you're trying to find a relatively rare protein in an experiment with many proteins...

...absolute quantitative analysis of a sample. Instead, relative quantification can be done using either the direct...identifies proteins solely by weight and size, relying on the signaling molecule solely asmeans of visualization...assays.  A brief note Since the indirect method relies on multiple secondary antibodies attaching to a...

...sequence (NGG, etc.) as well as their orientation relative to the gRNA (5’ or 3’). In some cases, there is... Links to relevant resources The homepage displays a graphic describing CasIDs and relevant properties... What are the features? CasPEDIA has collected relevant information on all the Cas enzymes you know and...

... present, it will repress expression of mKate2 relative to EBFP2. Weiss’s group used these reporters to... it inactive, thus inhibiting neurotransmitter release. How is this light-mediated? The researchers split...visualizing the location and function of microbes rely heavily on fluorescent protein genes. These optical...

...efficiency of Cas9 cleavage is relatively high while the efficiency of HDR is relatively low, meaning that most...Anzalone et al., 2019). PE3, as originally introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering mismatch...act first. PE4 and PE5, as originally introduced, rely on the PE2 enzyme. PEmax: Optimizing prime editor...

... generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail.... The advantages of this method are that it’s relatively simple to implement and doesn’t require time-...Overall, integrating viral vectors efficiently and reliably generate iPSCs, but they have several safety concerns...important control. Additionally, Sendai virus doesn’t rely on promoters for transcriptional regulation, so ...Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate the Stem...

...our engineers (Fig. 2). Creating this guide was relatively easy. I took a picture of virtually every container...each given container, when applicable Any other relevant information Prescribing an official name to all...important documentation, including SOPs or project-related documents, we define new and potentially ambiguous...transferable skills Find all of our science career related blog posts Read our science communication blog ...

...binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins containing...or function of a single protein? Oftentimes, they rely on protein purification. In this strategy a single...contrast, the polyhistidine tag is very small and rarely affects protein function (Bornhorst 2000).  One...other affinity purification methods where binding relies on a specific protein conformation, the polyhistidine-metal...questions about polyhistidine tags. For more antibody-related information, sign up to receive alerts for antibody...

...DREADDs are ideal for chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...