We narrowed to 262 results for: rigi

1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

   Type

   Blog Post

   Published

   March 5, 2019, 1:08 p.m.

   ...with Brainbow The Lichtman group developed the original iteration of this technology in 2007. Used to ...introduction to live cell imaging Learn more about the origin of the Brainbow system Visit our fluorescent proteins...

2. An Integrin Antibody Toolkit from IPI

   Type

   Blog Post

   Published

   March 28, 2024, 1:15 p.m.

   ...into two groups. Seven are reconstructions of the original hybridomas as new reproducible rat or mouse rAbs.... Based on the established applications of the original hybridoma monoclonals, we anticipate that our ...

3. Plasmids 101: Common Lab E. coli Strains

   Type

   Blog Post

   Published

   Nov. 7, 2014, 2:56 p.m.

   ...cloning and maintenance of a plasmids with R6Kγ origin; contains a mutant allele of the pir gene that ...organisms! Our Most Popular Plasmids 101 Posts: Origin of Replication The Promoter Region – Let's Go! ...

4. Technique: Probe Phage Genomes for Host Binding Proteins

   Type

   Blog Post

   Published

   Sept. 29, 2016, 2:30 p.m.

   ...receptor binding protein hit and can go back to the original plate to identify which phage gene encodes that...colony expressing a functional binding protein was originally located. Now you can grow up your colonies of...

5. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

   Type

   Blog Post

   Published

   May 18, 2018, 7:32 p.m.

   ...so-called “negative” results that conflict with our original hypotheses. Looking for novelty in our stories... presents only the results that align with the original hypothesis. Other practices that fall into this...

6. Plasmids 101: Dimers and Multimers

   Type

   Blog Post

   Published

   Jan. 10, 2023, 2:15 p.m.

   ...et al., 2009). Since multimers have additional origins of replication compared to monomers, they are also...  Plasmids 101: What is a plasmid? Plasmids 101: Origin of ReplicationPlasmids 101: PCR...

7. How to Design Your gRNA for CRISPR Genome Editing

   Type

   Blog Post

   Published

   Sept. 24, 2020, 1:15 p.m.

   ...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...control, one should revert the edit back to the original in order to prove that the phenotype was really...

8. 27 Hot Plasmids from 2016

   Type

   Blog Post

   Published

   Dec. 22, 2016, 3:03 p.m.

   ... Dr. Rod Devenish (both at Monash University) originally developed the system for monitoring and analysing...prototrophic strains. The vectors were based on the original EasyClone vectors (Jensen et al 2013) allow for... with an appropriate phosphatase, a technique originally pioneered by Markus Seeliger for the high-yield...

9. Important Considerations When Using AAVs

   Type

   Blog Post

   Published

   June 13, 2017, 2:30 p.m.

   ...which cell type? Location (3): Traveling from the original location - Axonal Transport in the CNS What tissue...your particular experiment! Traveling from the Original Location - Axonal Transport It has been demonstrated...

10. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...simplest and smallest bacteria. The absence of a rigid cell wall makes them resistant to antibiotics and...its ability to change shape and the absence of a rigid cell wall. Here, I will cover some of the best ways...

11. Typing CRISPR Systems

    Type

    Blog Post

    Published

    March 18, 2025, 1:15 p.m.

    ... and variants are typically based on species of origin. There are currently two classes, six types, 33...s0006297921100114 Koonin, E. V., & Makarova, K. S. (2019). Origins and evolution of CRISPR-Cas systems. Philosophical...

12. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...acceptors, then biarsenicals are a good option. Originally developed by Roger Tsien and co-workers (Griffin...compounds such as British anti-Lewisite (which was originally developed to treat heavy metal intoxication in...

13. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    Published

    Feb. 27, 2024, 2:15 p.m.

    ...you to quickly associate your plasmids with the original publication where you described them. Search for...available to the research community. This article was originally written by Tyler Ford in January 2016. It was...

14. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type

    Blog Post

    Published

    Dec. 20, 2017, 2:33 p.m.

    ...journal editor) of the work of those in your field. Original contributions to the field that have had a major... the same or allied academic field Evidence of original scientific or scholarly research contributions...

15. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type

    Blog Post

    Published

    Sept. 28, 2020, noon

    ...Originally published Mar 3, 2016 and last updated Apr 13, 2021 by Will Arnold. Although CRISPR systems...This pEcCas/pEcgRNA system works similar to the original pCas/pTargetF but has many other advantages including...

16. 15 Hot Plasmids from 2017

    Type

    Blog Post

    Published

    Jan. 9, 2018, 3:02 p.m.

    ...Tol2 Gateway-Compatible Toolbox is based on the original Tol2kit generated by the Chi-Bin Chien lab (Kwan... variant HF-Cas9. They subsequently tested the original BE3 and HF-BE3 using a gRNA with high off-target...

17. 22 Hot Plasmid Technologies from 2014

    Type

    Blog Post

    Published

    Jan. 6, 2015, 5:21 p.m.

    ...contextualized knowledge of their roles inside cells. The original APEX (enhanced ascorbate peroxidase) reporter ...their canonical positions (SP218, SP222) using the original or improved phosphoprotein synthesis reagents....

18. Antibodies 101 Series on TikTok

    Type

    Blog Post

    Published

    March 19, 2024, 1:15 p.m.

    ...antibodies #molecularbiology #science #scicomm ♬ original sound - Addgene We'll be posting these animations...

19. Meet Aavery, Blugene's New Virus Friend!

    Type

    Blog Post

    Published

    Feb. 13, 2020, 2:34 p.m.

    ...* Aavery! Welcome to Addgene, Aavery!       Original post: In December, we introduced you to Blugene...

20. Our New Antibody Mascot is....

    Type

    Blog Post

    Published

    Feb. 24, 2022, 2:15 p.m.

    ...unveil the winning name on Feb. 24. Stay tuned!    Original Blugene and Aavery would like your attention, ...