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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 135 results for: tTA

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Showing: 81 - 100 of 135 results

  1. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...canonical loxP sequence is ATAACTTCGTATA-GCATACAT-TATACGAAGTTAT. The loxP sequence does not occur naturally ...

  2. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...product is a mini-Y with both Fab regions still attached to one another. Size: ~110 kDa. Fab' - These fragments...

  3. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ...the Tuskegee syphilis experiment, the case of Henrietta Lacks, the Havasupai incident, and the sterilization...

  4. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ... elegans cell lines to express wrmScarlet1-10 unattached to any other cellular protein. They used CRISPR...

  6. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ... 13, 653-658 (2013). Dimitri, A., Herbst, F. & Fraietta, J.A. Engineering the next-generation of CAR T-cells...

  7. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...pattern and an auxin inducible degron (AID) sequence attached to your protein of interest. TIR1 interacts with...

  9. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T. (2019). CRISPR-Cas3...

  10. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...libraries may not have amplification protocols attached.  Final step: validation  Remember, there is ...

  11. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...self-cleave and the protease and degron remain attached to the tagged protein. In this event, the degron...

  12. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...transfer DNA (tDNA) into the genomes of the plants it attacks Growing the transformed agrobacterium culture Dipping...

  13. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...labels (green circles in figure 2 below) can be attached to each antibody. But being big is a double-edged...

  14. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ..., Chrétien S, Cartier N, Aubourg P, Fischer A, Cornetta K, Galacteros F, Beuzard Y, Gluckman E, Bushman...

  15. Plasmids 101: Protein tags

    Type
    Blog Post
    Published
    Dec. 11, 2014, 4:26 p.m.
    ...TAP GRRIPGLINP WKRRWKKNFI AVSAANRFKK ISSSGALDYD IPTTASENLY FQGEFGLAQH DEAVDNKFNK EQQNAFYEIL HLPNLNEEQR NAFIQSLKDD...

  17. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...sample (i.e. cultured cells or tissues).  Step 2: attach anchors to your sample that will link biomolecules...

  18. QC Sequencing Technologies at Addgene

    Type
    Blog Post
    Published
    July 15, 2025, 1:15 p.m.
    ...3). It uses (aptly named) “nanopores” that are attached to the flow cell membrane and have an electrical...

  19. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of tRNAs that...

  20. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ...Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that facilitate...
Showing: 81 - 100 of 135 results