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We narrowed to 105 results for: transfection

Showing: 81 - 100 of 105 results

  2. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...over for other applications, like PCR, cloning, transfection, or long-term storage. But what about those ...

  3. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...electroporation or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem...

  4. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...too young and should not be used for important transfection experiments right after they’ve been thawed....

  8. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...structures within cells. HeLa cells were transfected with plasmids encoding FPs tagged with the plasma...

  9. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...could interfere with interpreting the results of transfection assays. Go forth and perform your DNA extractions...

  10. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...protein of interest, such as a sample transiently transfected or stably infected with the protein of interest...

  11. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...restriction digest! Then, you can transform or transfect the cells that you want to use for your experiments...

  12. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple...

  13. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...optimizing this step, or consider enriching for transfected cells by flow sorting or drug selection, assuming...

  14. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...plasmid, and (3) the packaging plasmid. These are transfected into target cells which produce lentiviral particles...

  16. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...genome. While this may effectively serve as a transfection or transduction control, a different gRNA would...

  17. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... as early as 48-72 hours post-transformation/transfection in a transient assay. For stable transformation...

  20. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... With the ability to produce neuron specific transfection using Cre-lox, this new method could allow for...
Showing: 81 - 100 of 105 results