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We narrowed to 105 results for: transfection

Showing: 81 - 100 of 105 results

  2. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ...over for other applications, like PCR, cloning, transfection, or long-term storage. But what about those ...

  4. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...too young and should not be used for important transfection experiments right after they’ve been thawed....

  6. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...electroporation or poor cellular entry with other transfection methods. The Junwei Shi Lab addressed this problem...

  8. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...restriction digest! Then, you can transform or transfect the cells that you want to use for your experiments...

  9. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...structures within cells. HeLa cells were transfected with plasmids encoding FPs tagged with the plasma...

  10. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...could interfere with interpreting the results of transfection assays. Go forth and perform your DNA extractions...

  11. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...protein of interest, such as a sample transiently transfected or stably infected with the protein of interest...

  12. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...scientists use a multitude of techniques including co-transfection of two or more plasmids, the use of multiple...

  13. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...plasmid, and (3) the packaging plasmid. These are transfected into target cells which produce lentiviral particles...

  14. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...optimizing this step, or consider enriching for transfected cells by flow sorting or drug selection, assuming...

  16. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...genome. While this may effectively serve as a transfection or transduction control, a different gRNA would...

  17. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ... as early as 48-72 hours post-transformation/transfection in a transient assay. For stable transformation...

  19. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... With the ability to produce neuron specific transfection using Cre-lox, this new method could allow for...
Showing: 81 - 100 of 105 results