We narrowed to 259 results for: try

There are many ways to communicate your science - so here we're covering how to pick the best one for you.

July 1, 2022, 1:15 p.m.

Learn how to identify negotiation skills you already use in your lab and life and develop them further as a professional skill set.

March 3, 2022, 2 p.m.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Aug. 21, 2018, 12:31 p.m.

Having trouble with your transductions? Find troubleshooting tips from Addgene scientist Leila Haery.

Aug. 11, 2016, 2:23 p.m.

Colony PCR is a useful technique for quickly screening for successful constructs after a plasmid cloning experiment.

May 12, 2016, 2:30 p.m.

Tips to help you save time when using the the Basic Local Alignment Search Tool (BLAST) provided by NCBI to compare sequence results.

May 29, 2014, 1:29 p.m.

Our depositors don't just deposit great plasmids - they also do some incredible science! Check out some incredible depositor research stories from 2022.

Dec. 15, 2022, 2:15 p.m.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Oct. 4, 2018, 12:44 p.m.

Find tips on how to write your first review article - a useful experience for any scientist.

Feb. 16, 2017, 3:30 p.m.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Jan. 12, 2017, 3:30 p.m.

Welcome to the Addgene blog! Here you'll find all the info you need on plasmids, CRISPR, viral vectors, fluorescent proteins, science careers, and more.

Jan. 25, 2021, 5:54 p.m.

In our new Hot Plasmids segment on the Addgene podcast, you can quickly learn about a variety of new plasmid technologies from our quarterly newsletter.

May 17, 2017, 3:37 p.m.

We are excited to officially re-launch our redesigned AAV Data Hub, with easy submission and DOIs to help share science!

Feb. 7, 2023, 2:15 p.m.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Aug. 20, 2020, 2:30 p.m.

There are many method to identify protein-protein interactions. Learn about different method of proximity labeling based on biotin-streptavidin interactions.

Dec. 2, 2019, 2 p.m.

Learn more about unique organisms that are being developed as model systems in this blog post.

May 2, 2019, 12:41 p.m.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

April 7, 2016, 2:30 p.m.

Read our highlights from the Future of Research Symposium Boston 2015 and how scientists are tackling structural problems in academic research.

Nov. 25, 2015, 4 p.m.

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

April 9, 2015, 3:29 p.m.

Create a more sustainable lab by making small changes in your day-to-day or aim for long-term conservation goals. Find easy action items in the blog post.

April 22, 2019, 12:26 p.m.