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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 260 results for: uros

Showing: 81 - 100 of 260 results

  1. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ... Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969-4.   Increased neuronal expression...flexibility for in vivo proximity labeling Increased neuronal expression using Cre-mediated AAV Targeted Evolution...found at least two variants that display increased neuronal expression in the brain while having decreased...M-CREATE plasmids here!  Goertsen D, et al. Nature Neuroscience. 2022. https://doi.org/10.1038/s41593-021-00969...

  2. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...via airway administration. AAV11 can transduce neurons for retrograde labeling, making it a powerful tool... tool for circuit tracing and other neuroscience applications (Figure 4). Check out our blog post on AAV...enables potent retrograde labeling of projection neurons. Created with BioRender.com. Addgene has tested... Bentley While several viral tools can perform neuronal tracing, anterograde tracing poses challenges,...labeling across single synapses, a key attribute for neuronal tracing. Labeling was nontoxic and activity-dependent...

  3. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...the data is heavily dominated by the U.S. (42%), Europe (23%), and China (14%). Spikes in plasmid requests...majority of our plasmid orders come from the U.S. and Europe, we see a corresponding dip in plasmid request ...following hierarchical model: Because the U.S., Europe, and China account for over 75% of all of our plasmid...(the last date in our dataset) for the U.S. and Europe. To improve the stability of our model, we considered...scientists placing orders per day across regions, with Europe most impacted and China least impacted. The inferred...

  4. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...far-red genetically encoded indicators of neuronal activity. J Neurosci Methods 362, 109314 (2021). Yu, D. et... Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living mouse. Image courtesy...(2015). Shcherbakova, D.M., Stepanenko, O.V., Turoverov, K.K. & Verkhusha, V.V. Near-Infrared Fluorescent...improved monomeric infrared fluorescent protein for neuronal and tumour brain imaging. Nat Commun 5, 3626 (...

  5. What's New in CRISPR - December 2019

    Type
    Blog Post
    Published
    Dec. 17, 2019, 2:37 p.m.
    ...fluorescent tags in neurons. Their toolkit named ORANGE (Open Resource for the Application of Neuronal Genome Editing...CRISPR/Cas9-based toolkit for epitope tagging in neurons Harold MacGillavry’s lab developed a CRISPR-based...targeted genomic integration of fluorescent tags in neurons.  Read the paper in bioRxiv Find the ORANGE toolkit...

  6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ... in Frontiers in Neuroanatomy and summarized some considerations for precise neuronal targeting using ...Considerations For Precise Neuronal Targeting Using AAV Technologies Using AAV for neuronal tracing Resources...

  7. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...ion-based communication is not just for neurons. Non-neuronal cells do this too. It's just that for non-neural...you hear the phrase “action potential?” Firing neurons are likely the first thing that comes to mind. ...exhibits an inactivation maximum of 590 nm light. Aurora and Phobos optogenetic tools - A collection of ...

  8. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... expression.  Cloning and expressing RNAi pMKO.1 puro (Plasmid #8452) RNA interference (RNAi) is an RNA-mediated...Vectors    Expressing your gene of interest pLenti-puro (Plasmid #39481) You can clone your gene of interest....1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This is a lentiviral...Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. No time to produce virus...

  9. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...Since the pioneering days of Golgi and Cajal, neuroscientists have developed and refined many tools to tease...understand the complex interworking of the 86 billion neurons in the human brain. But studying synaptic connections...generally very small, and can contain different neurotransmitters. Using genetically encoded fluorescent proteins...inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind to the endogenous synaptic proteins...

  10. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...Brain Research Through Advancing Innovative Neurotechnologies (BRAIN) Initiative Cell Census Network, the... Fig. 1: Antibody production methods    NeuroMab and others have an extensive library of monoclonal...mouse tissue and compare the IHC results to both NeuroMab and the Allen Brain Atlas. The comparison with...intuitive guide for checking their staining and neuroanatomy without the fear of referencing a wrong section...

  11. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...in green monkeys and cultured human neurons, with broader neuronal expression than AAV9. This new capsid...https://doi.org/10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate...-brain barrier and delivering a transgene, with neuron bias observed in young macaques and vascular bias...

  12. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    Published
    Sept. 17, 2015, 2:30 p.m.
    ...channelrhodopsin for activation of CNS neurons. Current protocols in neuroscience / editorial board, Jacqueline... Simon. The surgeries and standard molecular neuroscience validation experiments we discussed last week...optogenetic molecules in freely moving mice. Neuroscience research. 2011;70(1):124-7. doi: 10.1016/j.neures...

  13. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... rare genetic eye disease, Leber’s congenital amaurosis. This is the first of what I, scientists, doctors...childhood-onset form of blindness name Leber’s Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 ...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and ...muscular dystrophy, spinal muscular atrophy, neurodegenerative diseases and metabolic diseases are also underway...

  14. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    Published
    Dec. 3, 2019, 11:38 a.m.
    ...expressing validated antibodies for neuroscience research (see the NeuroMab Collection for details). The following..., I wanted to find a specific plasmid from the NeuroMab collection for an antibody against PARIS, a zinc...zinc finger protein involved in neurodegeneration in Parkinson’s disease. Knowing the primary investigator...

  15. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...Domains Tweeted by Biologists. 7. Biologists, neuroscientists and ecologists (re)tweet scientific domains...Domains, Categorized by Discipline. Biologists, neuroscientists, and ecologists (orange box) post tweets containing...however, ~20% of life scientists  (biologists, neuroscientists, ecologists) tweet scientific domains 20% or...

  16. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...iPSCs) to take on a “neuron-like” phenotype when targeting genes involved in neurogenesis, which had proven...EGFP, mCherry, iRFP, tdTomato, luciferase, LacZ, puromycin or CreERT2 can be combined with custom-cloned ...a separate reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral... voltage indicator for use in the detection of neuronal action potentials. The Cohen lab wished to improve...the genome. In this work, they use AAVS1_Puro_PGK1_3xFLAG_Twin_Strep and nuclease driven recombination ...

  17. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...effective at editing cytosines preceded by guanine (Thuronyi et al., 2019). Following the development of adenine...high product purity and low off-target editing Thuronyi et al., 2019 evoAPOBEC1-BE4max   Improved editing... K. T., Eton, E., Lapinaite, A., Newby, G. A., Thuronyi, B. W., Wilson, C., Koblan, L. W., Zeng, J., Bauer...891. https://doi.org/10.1038/s41587-020-0453-z Thuronyi, B. W., Koblan, L. W., Levy, J. M., Yeh, W.-H....

  18. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...image the calcium response in the dendrites of T5 neurons by placing the calcium indicator GCaMP6f in the...optogenetically control the depolarization of R2 neurons using CsChrimson::tdTomato, an optogenetic tool...projection-specific brain-wide reconstruction of single neurons. Nature Methods 15:1033–1036 . https://doi.org/...

  19. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...heard of a Freshers Fair? If you’re from outside of Europe, the answer is probably no, but these fun and informative... of the main ways budding young scholars across Europe get introduced to the cultures and opportunities... and scientific community activities throughout Europe and the world. So get in touch! Resources at Addgene...

  20. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ...Technologies and Methods for Targeted Neuronal Manipulation. Front Neuroanat 13: . https://doi.org/10.3389/fnana...expression, while the human synapsin 1 promoter is neuronal specific (Haery et al., 2019). Most AAV transfer...
Showing: 81 - 100 of 260 results