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Addgene

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We narrowed to 842 results for: NTS

Showing: 491 - 500 of 842 results

  1. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...particularly related to the neurosciences.  These developments in microscopy coupled with techniques that make...view of a cleared rat pons, medulla and the first segments of the spinal cord showing the cortico spinal ...spinal tract, CST, spinal cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the human...anterior, P= posterior, D= dorsal, V= ventral Developments in light sheet fluorescence microscopy Modern...

  2. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    Published
    Jan. 16, 2020, 2:18 p.m.
    ...require a standard or a reference, meaning saving on reagents (and time)! AAV titering using droplet digital...emulsion technology. Each droplet contains the ingredients for amplification of the target DNA. This partitioning...prepare your sample dilutions. Aliquot all of your reagents into single-use tubes and grab a fresh one for...digital PCR is becoming a popular option for PCR experiments.  References Furuta-Hanawa, Birei, Teruhide...

  3. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...is the first of what I, scientists, doctors and patients hope are many more clinical successes for gene...the human condition. Luck brought all these key elements together at the right time and place. But it is...limitation, with limited success so far. Luckily for patients, this gene can be targeted to RPE cells using ...improve the technology: engineering of new capsid variants, designing expression cassettes to regulate and...

  4. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

    Type
    Blog Post
    Published
    Dec. 2, 2014, 7:23 p.m.
    ...give to post-docs and graduate students as they complete their appointments in your lab?  Fujii: These days... makes it much easier to knock-in LexA binding elements into the target region. However, it struck me ...Throughout your career, you have held research appointments in the US (New York University School of Medicine...

  5. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ... type of memory which includes autobiographical events (see Jin & Maren, 2015). Directional flow of information... and memory retrieval. In neuronal tracing experiments, brain tissue is injected with compounds that...injection or iontophoresis (applying small electric currents via inserted electrodes) may be applied, but these...proteins VP1, VP2, and VP3. To date several hundred variants, or serotypes, have been found naturally or have...like these may be a starting point for future developments in the field of AAV vectors. Conclusion An ideal...

  6. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...significant, negative effects in RNA targeting experiments. If Cas7-11 lacked this toxicity, it would represent...forward for CRISPR-based RNA editing - and further experiments strongly supported their initial findings.  The... approach to gene replacement therapy for most patients. But how does one go from “cut and paste” to “...Programmable Addition via Site-specific Targeting Elements (PASTE). Finding the right integrase for this ...

  7. Transferable Skills Guide: Creativity

    Type
    Blog Post
    Published
    Jan. 18, 2022, 2:15 p.m.
    ...exercise: Consider the issue as if there were no restraints on your problem-solving resources. What would...which people were asked to create a device to help students during an interview. When they were instructed... ideas heard.  So how can we assure that all participants feel comfortable sharing their ideas? And how...have plans in place to be able to continue our experiments. Thinking about these plans before you’ve run...

  8. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...specific QC experiments performed varies for each viral lot. To learn which specific QC experiments were performed...ddPCR) using primers and probes targeting the ITR elements and an internal control of known titer (protocol...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...

  9. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    Published
    Nov. 21, 2024, 10:47 p.m.
    ...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...any conference is scientists, from techs to grad students to professors, stopping by to say, “Everything...— in their classroom, in their lab, in their experiments, to enable virtual learning during the pandemic...

  10. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...annealing can be affected by the presence of protein contaminants, which is a factor in AAV titration since the...repeating the exact same PCR twice can give titer measurements that vary up to two-fold. How can we get more...low levels of background protein and chemical components, ddPCR may not be an improvement over qPCR (Taylor...). What do Addgene AAV titers mean for your experiments? Because (physical) AAV titers are approximations...
Showing: 491 - 500 of 842 results