We narrowed to 855 results for: NTS

...labeling of neurons for tracing experiments.  Neuronal tracing experiments: While synthetic promoters with...the retina, it was more likely to have borrowed elements from a natural promoter that was active in the...labeling would be useful for neuronal tracing experiments where lower levels of labeling makes it easier...

... survive in competitive environments — like by living where no one else wants to. Some organisms manage...their host organisms to thrive in competitive environments. The genes encoding these functions form the...cryptic plasmid is among the most numerous genetic elements in the human gut. Cell, 187(5), 1206-1222.e16....

..., or universities may have someone senior from accounts or HR on the judging panel. It’s important for...Skills 5. Practice makes perfect, apply for small grants too There are actually quite a lot of different... researchers, so look into applying for smaller grants from within your institute or a private funding...the experience I gained in writing small travel grants to attend conferences and a seed funding grant ...

...companies and organizations selling biological reagents have a ton of data on the catalog page. It’s not.... Although many may not realize it, all of the reagents available at Addgene come from researchers, and...don’t have the resources to recapitulate the experiments performed by our thousands of depositors. That...Google Scholar, which searches scientific articles, patents, and even some doctoral dissertations. Streamline...

... it can amplify DNA using only a short list of reagents and several heating and cooling steps. PCR relies...strand of DNA to add nucleotides to.  Using these reagents and a series of heating (denaturing) and cooling...cycle. However, it is common for dNTPs and other reagents to run low during the final cycles which would...of different molecular biology techniques and experiments. End point PCR’s goal is to amplify a specific...

...to develop therapies that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing... process can be a bit of a mystery to graduate students in academia. Getting a drug to the market usually...pages. In academia, however, writing papers and grants is an inescapable and essential part of being able...’re often required to at least attempt to write grants. If you don’t enjoy or excel at writing, an industry...

...microbiology meeting. Create an outline: Write key bullet points for what you would like to say to help build your...result, and the fundamental takeaway. Writing these points will help you remember what to say and provide ...outline you created for yourself. Hit the key bullet points and elaborate if your audience seems captivated... the project as a whole. In addition to time constraints, there may be particular poster you are interested...

... corrective amounts of protein for any such disease. This technology therefore represents a potential ...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423...doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT (2019) Long-...

...the degron domain is terminal. Between the tag elements (protease and degron) and the protein of interest...other systems reviewed here. The only required components are 1) a SMASh-tagged protein and 2) asunaprevir...reviewed here. The AID system relies on three components. First, the protein of interest must be AID-tagged...SMASh). If you don’t want to deal with additional components beyond the minimum required for a degron system...

...produced in multiple different cell types, including plants, mammals, and insects. In this post, we will compare...producing virus within 24 to 48 hours. Harvesting timepoints will depend on your specific virus, so this is...methods like ultracentrifugation, while lentivirus supernatants are centrifuged and filtered to eliminate any... important to you — virus type, cost, time, or reagents and equipment. Hopefully this post has provided...