We narrowed to 855 results for: NTS

... corrective amounts of protein for any such disease. This technology therefore represents a potential ...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423...doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT (2019) Long-...

...number of cycles.  Choosing a method  As with all experiments, a number of factors will determine which approach...Resources needed Most molecular biology labs and departments will have qPCR machines available for use. ddPCR...Chung WK. Quantitative analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc... The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative...

...particularly related to the neurosciences.  These developments in microscopy coupled with techniques that make...view of a cleared rat pons, medulla and the first segments of the spinal cord showing the cortico spinal ...spinal tract, CST, spinal cord and cranial nerve afferents labelled with a AAV8 expressing eGFP under the human...anterior, P= posterior, D= dorsal, V= ventral Developments in light sheet fluorescence microscopy Modern...

...require a standard or a reference, meaning saving on reagents (and time)! AAV titering using droplet digital...emulsion technology. Each droplet contains the ingredients for amplification of the target DNA. This partitioning...prepare your sample dilutions. Aliquot all of your reagents into single-use tubes and grab a fresh one for...digital PCR is becoming a popular option for PCR experiments.  References Furuta-Hanawa, Birei, Teruhide...

...is the first of what I, scientists, doctors and patients hope are many more clinical successes for gene...the human condition. Luck brought all these key elements together at the right time and place. But it is...limitation, with limited success so far. Luckily for patients, this gene can be targeted to RPE cells using ...improve the technology: engineering of new capsid variants, designing expression cassettes to regulate and...

...give to post-docs and graduate students as they complete their appointments in your lab?  Fujii: These days... makes it much easier to knock-in LexA binding elements into the target region. However, it struck me ...Throughout your career, you have held research appointments in the US (New York University School of Medicine...

... type of memory which includes autobiographical events (see Jin & Maren, 2015). Directional flow of information... and memory retrieval. In neuronal tracing experiments, brain tissue is injected with compounds that...injection or iontophoresis (applying small electric currents via inserted electrodes) may be applied, but these...proteins VP1, VP2, and VP3. To date several hundred variants, or serotypes, have been found naturally or have...like these may be a starting point for future developments in the field of AAV vectors. Conclusion An ideal...

...sequencing and bioinformatic technologies are used.  Improvements to sequencing technologies and bioinformatic... lot of effort to stay up-to-date with these improvements. In this post, we discuss the sequencing technologies...addition, DNA synthesis stops. The resulting DNA fragments are pooled and then separated by size using electrophoresis...depositor-provided sequences to confirm the correct plasmid components. For more information on this process, see our...

...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...any conference is scientists, from techs to grad students to professors, stopping by to say, “Everything...— in their classroom, in their lab, in their experiments, to enable virtual learning during the pandemic...

...specific QC experiments performed varies for each viral lot. To learn which specific QC experiments were performed...ddPCR) using primers and probes targeting the ITR elements and an internal control of known titer (protocol...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...