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We narrowed to 842 results for: NTS

Showing: 501 - 510 of 842 results

  1. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    Published
    Oct. 20, 2016, 2:30 p.m.
    ...questions and comments go up early in the day, and the moderators were on top of any comments that were at...Anything”) on r/Science. You can see some of Joanne’s comments on the AMA process below, but we also wanted to...Protocols: Using Apps to Save Time & Track Your Experiments Additional Resources See How We're Sharing ...

  2. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...biorepositories or have been directly isolated from patients. In these cases, a test known as Short Tandem ... the lower the passage number the better for experiments. This is to ensure the cells are as close to ...original Cell line you purchased, keeping all experiments carried out on low passage cells and this means...and we can progress faster to desired clinical endpoints.   Nick Amiss is an ATCC Outreach Scientist at...

  3. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ...smaller fragments of the transgene have been seen with the hybrid method. While these fragments are likely...co-infected with both part A and part B AAVs, the fragments reassemble and produce the full-length gene. Below... donors for two consecutive CRISPR-mediated HDR events which results in the integration of a larger donor...

  4. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...have considered myself neutral in any of their disagreements. In fact, conflict resolution isn’t just about...also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive...constructive) conflict. As you perform the experiments to address a reviewer’s concerns, you are making... around missed tasks, stalled projects, and disagreements over prioritization. The source of those conflicts...

  5. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...fun is building within the constraints given. And there were many constraints during the COVID-19 pandemic...talking to their webcams.  Build: I sent the participants interview questions they could answer, and I...remote filming. Learn: There were still a few adjustments I wanted to make to ensure smooth filming in ...

  6. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if...target time frame.   I suggest that all graduate students and postdocs form peer mentoring groups to practice... and data professionally). Do remember the fine points of visual aids. Here’s a great slideshare with ...sharing model works. See if you can elicit helpful comments from your audience as in “Does anyone else have...

  7. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...months. With CRISPR (B), you can inject CRISPR components directly into a zygote and obtain a knockout ...complexes of Cas9 protein and the separated guide RNA components (crRNA and tracrRNA) for maximum Easi-CRISPR ...gRNAs. As with other homology-directed repair experiments, you’ll want the guide cut site to be as close... simple method for delivery of genome editing components to the germline. In contrast to standard microinjection...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...about are (1) your experimental conditions and measurements; (2) the cells you’re targeting; (3) how much...and then test each vector before starting your experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger..., at an adequate level for your experimental measurements. The number itself is determined through validation...  Many other factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors...

  9. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...parallel between the timelines of the scientific advancements and the noteworthy work of fiction that it inspired...genetics was booming, SciFi saw the rise of literary giants such as Isaac Asimov, Arthur C. Clarke, James Blish... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...

  10. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...are often little tricks that are essential for experiments but that nobody talks about. After months of ...A549 cells to express Cas9. In these particular experiments, we generated A549 cells stably expressing Cas9...good gel image. If you are analyzing short DNA fragments, run PAGE instead of agarose to get sharper bands... quick and easy it is to share information and regents, it’s exciting to think that no matter who you ...
Showing: 501 - 510 of 842 results