We narrowed to 855 results for: NTS

...specific QC experiments performed varies for each viral lot. To learn which specific QC experiments were performed...ddPCR) using primers and probes targeting the ITR elements and an internal control of known titer (protocol...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...

...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...any conference is scientists, from techs to grad students to professors, stopping by to say, “Everything...— in their classroom, in their lab, in their experiments, to enable virtual learning during the pandemic...

...annealing can be affected by the presence of protein contaminants, which is a factor in AAV titration since the...repeating the exact same PCR twice can give titer measurements that vary up to two-fold. How can we get more...low levels of background protein and chemical components, ddPCR may not be an improvement over qPCR (Taylor...). What do Addgene AAV titers mean for your experiments? Because (physical) AAV titers are approximations...

...questions and comments go up early in the day, and the moderators were on top of any comments that were at...Anything”) on r/Science. You can see some of Joanne’s comments on the AMA process below, but we also wanted to...Protocols: Using Apps to Save Time & Track Your Experiments Additional Resources See How We're Sharing ...

...biorepositories or have been directly isolated from patients. In these cases, a test known as Short Tandem ... the lower the passage number the better for experiments. This is to ensure the cells are as close to ...original Cell line you purchased, keeping all experiments carried out on low passage cells and this means...and we can progress faster to desired clinical endpoints.   Nick Amiss is an ATCC Outreach Scientist at...

...smaller fragments of the transgene have been seen with the hybrid method. While these fragments are likely...co-infected with both part A and part B AAVs, the fragments reassemble and produce the full-length gene. Below... donors for two consecutive CRISPR-mediated HDR events which results in the integration of a larger donor...

...have considered myself neutral in any of their disagreements. In fact, conflict resolution isn’t just about...also be good. In science, a reviewer’s critical comments on your manuscript is a form of (hopefully constructive...constructive) conflict. As you perform the experiments to address a reviewer’s concerns, you are making... around missed tasks, stalled projects, and disagreements over prioritization. The source of those conflicts...

...fun is building within the constraints given. And there were many constraints during the COVID-19 pandemic...talking to their webcams.  Build: I sent the participants interview questions they could answer, and I...remote filming. Learn: There were still a few adjustments I wanted to make to ensure smooth filming in ...

...products that resulted from their research, “aha” moments, and future research directions. Don’t worry if...target time frame.   I suggest that all graduate students and postdocs form peer mentoring groups to practice... and data professionally). Do remember the fine points of visual aids. Here’s a great slideshare with ...sharing model works. See if you can elicit helpful comments from your audience as in “Does anyone else have...

...about are (1) your experimental conditions and measurements; (2) the cells you’re targeting; (3) how much...and then test each vector before starting your experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger..., at an adequate level for your experimental measurements. The number itself is determined through validation...  Many other factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors...