We narrowed to 855 results for: NTS

...about are (1) your experimental conditions and measurements; (2) the cells you’re targeting; (3) how much...and then test each vector before starting your experiments (Rolland et al., 2016; Su et al., 2004; Taschenberger..., at an adequate level for your experimental measurements. The number itself is determined through validation...  Many other factors can also affect your AAV experiments. Luckily, the other posts in our Viral Vectors...

...are often little tricks that are essential for experiments but that nobody talks about. After months of ...A549 cells to express Cas9. In these particular experiments, we generated A549 cells stably expressing Cas9...good gel image. If you are analyzing short DNA fragments, run PAGE instead of agarose to get sharper bands... quick and easy it is to share information and regents, it’s exciting to think that no matter who you ...

...parallel between the timelines of the scientific advancements and the noteworthy work of fiction that it inspired...genetics was booming, SciFi saw the rise of literary giants such as Isaac Asimov, Arthur C. Clarke, James Blish... that revolves around synthetic humans called replicants which are genetically engineered by a powerful...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...

.../error, variance, data ranges. how closely data points fall to each other (see Fig. 1). Alpha values...certain information about. The assumptions and requirements that need to be met for a parametric test are... - Quantitative If your data doesn’t fit the requirements above, a non-parametric test may be the way ... were additive, etc.). ANCOVA and MANCOVA All variants of ANOVAs discussed so far assume that the independent...

...AAV experiments to the Data Hub. Submit your data by Nov. 12 to compete. The top ten participants will...winner each receiving a $500 travel award. All participants will receive an Addgene t-shirt.  Our judges...

...capture in these types of samples is their fast movements. Depending on the sample, a temporal resolution...millisecond scale may be needed. Most commonly these experiments are performed on a fluorescent widefield microscope...your institution when heading into a new set of experiments. Many thanks to our guest blogger, Doug Richardson...Choose the Brightest Fluorescent Protein for Your Experiments Resources on Addgene.org Browse Our Fluorescent...

...CRISPR tools for use in plants.  Listen to the segment on CRISPR tools for plants. Special thanks to our...enhancing iPSC production, and CRISPR tools for plants. You can find additional hot plasmids in our quarterly...

...scientific communities and welcome suggestions in the comments.  X has moved significantly away from the functionality... Every community benefits from a diversity of viewpoints, and we acknowledge there may be a variety of...invite you to follow us across our social media accounts.  -Addgene   ...

... Lab tip #2: Tube flick trick When combining reagents in the lab, it is important to mix them thoroughly...before use.  For efficient mixing, add the various reagents to a single tube and simply flick the tube several...everything Use barcodes and scanners to keep track of reagents in your lab. With barcode tracking, you will always...

...removed the uracil glycosylase inhibitor (UGI) components, which inhibit BER during cytosine-to-uracil ...developed two fusions of a CBE with other BER components: the DNA repair protein rXRCC1, or the DNA binding...both E. coli and C. elegans could be effective components of CGBEs. Their eOPTI-CGBE and cOPTI-CGBE had...they expanded CGBE target scope by creating BE variants with other Cas9s that recognize different PAMs...