We narrowed to 855 results for: NTS

... strong as the basis for many genome editing experiments. So, without further ado, the most request plasmid...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...

... be used for A•T to G•C edits in protoplasts or plants of rice and wheat. Read the paper in Genome Biology...cloning plasmids Mobile-CRISPRi allows CRISPRi experiments in diverse bacteria Carol Gross’s lab recently...can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration...

...tools from depositors who quickly shared their reagents: detection methods, synthetic nanobodies, SARS-CoV...began working with tech transfer offices to make reagents available to scientists in both academia and industry...Chromoproteins: Colorful Proteins For Molecular Biology Experiments by Jennifer Tsang Chromoproteins are a subset...

...Joanne's Reddit AMA Here they are annotated with my comments: Everyone on the team talks and listens in roughly...to get, as well as give, information to make improvements. Members connect directly with one another...in meetings, via email, at formal communications events or at informal gatherings, like at lunch. Many ...

...competition featuring a selected ingredient. Anyone who wants to participate brings in a dish that features that... for our website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success...would be a wonderful tradition for university departments to establish, with the goal of fostering better...

...This represents a considerable barrier to furthering research and developing effective treatments for ...dozens of wild-type genes and their associated mutants in other model systems. It is possible that a given...

...scientists quickly find much-needed phages for patients.   A phage directory is crucial because it's hard...Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires, it's likely that...in the works. To keep up to date with the new developments at Phage Directory and in the phage field, subscribe...

...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ... may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that GFP can ...enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well as a wide ...

...selectable gene product for isolation of successful transformants. Addgene distributes several ready-to-use, modular...specific filter sets and experimental output requirements. Select from these yeast-optimized fluorophore...vectors for your single or multi-color imaging experiments. Interested in epitope tags? Others may be interested...

...immediately clear that Addgene wanted to ensure applicants understood what the expectations were for the...time. This made time for me to attend to other commitments, without feeling guilty about missing work. As...Northwestern University. She is one of several students completing their co-op at Addgene. This is her...