We narrowed to 842 results for: NTS
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TypeBlog PostPublishedJune 2, 2020, 1:15 p.m....in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin...high throughput screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical...iodine (PI), allowing for calcineurin activity measurements in stressed or aging cell populations. ...
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Celebrate Open Data Day with Addgene’s AAV Data Hub!
TypeBlog PostPublishedMarch 5, 2020, 2:15 p.m....find details they need for planning their next experiments using a specific AAV tool. They can search the...tools. This word cloud (using wordart.com) represents contributed data on brain injection site from... a guide for designing your future injection experiments. Have your data or lab notebook handy? Contact... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog PostPublishedSept. 14, 2021, 1:15 p.m....RecA and RecBCD in bacteria. When two repeating elements love each other very much undergo recombination...sites, and other plasmids that contain repeating elements. Knowing how to amplify recombination-prone plasmids...save you the headache of troubleshooting failed experiments. Pick the right strain While common E. coli strains... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....reverse, making it unsuitable for some types of experiments. (To get more information on AraC and other promoter... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between... can do all of these more spatially confined experiments as well as those requiring reversibility that... -
Multifunctional Neural Fibers: Modulation from Gut to Brain
TypeBlog PostPublishedAug. 31, 2023, 1 p.m....hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher. ...control – this is important later! Next, additional components are added - the ‘bells and whistles’ – including...on the other hand, was the wild west for fiber implants. Due to the soft nature of the intestines and ... -
Addgene's Top 10 Blog Posts of 2015
TypeBlog PostPublishedDec. 7, 2015, 6 p.m.... Getting a lot of empty vector contaminants in your cloning experiments and tired of having to PCR many...the coming years as we stay on top of the many developments in science and the plasmids, technologies, and... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....of their luciferase assay, they concluded that components in the buffer used for protein purification must... expression would require multiple transduction events. For these reasons, iTOP is best suited to binary...small molecule NSDB and Na+ hypertonicity are key elements of iTOP. iTOP can be used to transduce Cas9 and... -
Celebrate National Book Month with Addgene
TypeBlog PostPublishedOct. 3, 2019, 12:47 p.m....relationships between microbes and insects, mammals, and plants, among others. I particularly enjoyed the emphasis... their paths cross with each other and with the events of the times." - Aliyah Weinstein, Marketing...community know what your favorite book is in the comments! Additional resources on the Addgene blog Learn... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....Recombinases And more! Control AAV For many AAV experiments, we recommend validating the serotype in your...vectors to more serotypes. We currently package four variants of these biosensors, which are optimized for specific... should improve the specificity of influence measurements by reducing photostimulation of the axons and... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....a degron, a domain that induces degradation. In plants, auxin binds to TIR1 of the SCF-TIR1 ubiquitin ...versatility of the engineered SpCas9-NG and its variants can be used to engineer specific sites that were... factors developed thus far for these latter experiments, such as transcription factors tethered to dCas9...