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We narrowed to 855 results for: NTS

Showing: 561 - 570 of 855 results
  1. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...biological sciences and constitute foundational elements of biomedical research. Traditional restriction...purification, and, most importantly, excessive time requirements. FastCloning is a simpler yet reliable cloning...directly into chemically competent E. coli and transformants selected with the appropriate antibiotic. Although...
  2. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ... variety in their job. Good relationships with clients and colleagues Sometimes this means you have to... in mind at all times when making decisions, assignments and polices. Sometimes the boss has to be the...knowledge-intensive workers”. I have added a few comments of my own to this list. Need feedback on their...
  3. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...to reuse plasticware or think about conserving reagents and buffers.  However, I had very environmentally... using.  I started to do little things to save reagents and money.  This was the start of my interest ...ethidium bromide, less stop buffer and miniscule amounts of plasmid sample.  How satisfying?  With practice...
  4. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher. ...control – this is important later! Next, additional components are added - the ‘bells and whistles’ – including...on the other hand, was the wild west for fiber implants. Due to the soft nature of the intestines and ...
  5. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...in turn binds to calcineurin-dependent response elements (CDREs) and drives gene expression. Because calcineurin...high throughput screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical...iodine (PI), allowing for calcineurin activity measurements in stressed or aging cell populations. ...
  6. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    Published
    March 5, 2020, 2:15 p.m.
    ...find details they need for planning their next experiments using a specific AAV tool. They can search the...tools. This word cloud (using wordart.com) represents contributed data on brain injection site from... a guide for designing your future injection experiments. Have your data or lab notebook handy?  Contact...
  7. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type
    Blog Post
    Published
    Sept. 14, 2021, 1:15 p.m.
    ...RecA and RecBCD in bacteria. When two repeating elements love each other very much undergo recombination...sites, and other plasmids that contain repeating elements. Knowing how to amplify recombination-prone plasmids...save you the headache of troubleshooting failed experiments. Pick the right strain While common E. coli strains...
  8. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...reverse, making it unsuitable for some types of experiments. (To get more information on AraC and other promoter... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between... can do all of these more spatially confined experiments as well as those requiring reversibility that...
  9. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... Getting a lot of empty vector contaminants in your cloning experiments and tired of having to PCR many...the coming years as we stay on top of the many developments in science and the plasmids, technologies, and...
  10. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...of their luciferase assay, they concluded that components in the buffer used for protein purification must... expression would require multiple transduction events. For these reasons, iTOP is best suited to binary...small molecule NSDB and Na+ hypertonicity are key elements of iTOP. iTOP can be used to transduce Cas9 and...
Showing: 561 - 570 of 855 results