... best bet is the Plasmid Map: We auto-generate a plasmid map based off of the plasmid sequence and feature...colleagues. Top of the plasmid page The ‘breadcrumb’ trail at the top of any plasmid page gives the hierarchy... to the individual plasmid page. The depositing lab page contains all the plasmids from the same lab while...contains all the plasmids deposited from the same publication. Next up, we have the plasmid name: This is...other plasmids with similar names in the collection, but you can use this name to find this plasmid from...A quicker way to find this plasmid through search is... The Addgene plasmid number: This is the unique... given to every plasmid in our catalog - if you know the catalog number of the plasmid you are searching...

... here at Addgene – 500,000 plasmids shipped! That's a 1/2 million plasmids sent to happy scientists around... you all the plasmids you need. We're focused on our mission – documenting your plasmids, storing them...500,000 plasmids go? Far and wide – that's where! Check out the map below which depicts where plasmids have...Sidney Street. Then the plasmids travel to labs around the world. We've shipped plasmids to more than 90,000...providing quality control and customer support, making plasmids easy for you to find and request in our online...shipping them to you quickly.   So, what does plasmid shipping look like at Addgene? Here's an inside... workings in Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture format...

...previous posts for our Plasmids 101 series, we examined a number of important plasmid elements – promoters...Resources on Addgene.org Find Cre plasmids Find loxP plasmids  ... expression proceeds. One popular lox-stop-lox plasmid is from Tyler Jack's lab: Lox-Stop-Lox TOPO.  Cre-dependent...  plasmids 101...

...localization, or protease protection. Thus far Plasmids 101 has covered GFP and its related fluorescent... however, those are just one (admittedly large) class of common fusion protein tags. Biochemists and molecular...stability activity, can be readily purified using plasmids found in this paper (available at Addgene).   ...interest: Which Fluorescent Protein Should I Use? Plasmids 101: Green Fluorescent Protein (GFP) Fluorescent...   plasmids 101...

...with a plasmid that already has the multicistronic element and reporter cloned in. In these plasmids you ...interest. Some of the plasmids listed in the table above (and their related plasmids) are designed to have... a one-stop reference guide for plasmids. We've combined our Plasmids 101 blog posts and additional resources...desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid lovers and ... an introduction to plasmids, allowing you to spend less time researching plasmid basic features and spend...May 11.) PubMed.   Read more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular biology...techniques including co-transfection of two or more plasmids, the use of multiple or bidirectional promoters...

...More: Plasmids 101: Antibiotic Resistance Genes Plasmids 101: Origin of Replication All Plasmids 101 Articles...Although plasmids do not naturally exist in mammals, scientists can still reap the benefits of plasmid-based...their genomes. Unlike plasmids used for expression in yeast and bacteria, plasmids used for stable transfections...Expression Plasmids? Check Out Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone...origin of replication (ORI) will not allow for plasmid replication in mammalian cells, for example, and...In this blog post we will discuss how mammalian plasmids differ from their bacterial counterparts, including...transfected cells. Before getting into the mammalian plasmid components, it may be useful to describe the means...

...types of yeast plasmids are defined below: Yeast Integrating plasmids (YIp): These plasmids lack an ORI...our first few Plasmids 101 posts, we focused mainly on the elements required for plasmid maintenance within...not only the number of plasmids found in each cell, but also whether the plasmid gets integrated into the...integration. Yeast Episomal plasmids (YEp): These are most similar to bacterial plasmids and are considered “...simple gene replacement/mutation. Yeast expression plasmids used in the lab typically contain all the necessary...how the regulation of bacterial ORIs determines plasmid copy number within the bacterial cell. Similarly...included within a yeast vector determine how the plasmid is replicated and maintained within the yeast cell...

...your plasmid; separate plasmid DNA from unwanted cellular “debris”; and then clean-up the plasmid DNA ...debris from the plasmid, and then clean-up the plasmid so that you are left with pure plasmid DNA. Image created...post-consumer recycled plastic Reduced plastic in buffer bottles Fewer plastic consumables Fewer pipette... help you choose the plasmid prep kit that is right for you. What type of plasmid prep do you need? As...As we covered in a previous post on plasmid preps, not all plasmid preps are the same. Your downstream...arise during the plasmid purification process. One is the possibility of damaging the plasmid DNA itself, ...carrying over contaminants from the plasmid prep process into the final plasmid eluate. Possible contaminants...

...for your work. Species Antibody Subclass Nanobody Addgene Plasmid Rabbit N/A* TP897 pTP1183 (1x... one IgG subclass.   Express the Nanobody In Bacteria: Table 2 also has a link to the plasmid you’ll need... How to Use the Nanobody Toolbox Find Nanobody Plasmids Structural comparison As the name suggests, ...different species, or at least from different IgG subclasses, so that secondary antibodies exclusively recognize...Must use different species and/or different IgG subclasses of 1° for multi-color staining Can be expensive...limited cross-reactivity between different IgG subclasses and different species, nanobodies are useful ...require using primary antibodies from different IgG subclasses. Instead, you can pre-incubate individual primary...

...example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors...reason, the top requested plasmid of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’...Plasmid technologies are constantly evolving, but sometimes a technology is so useful it forever enhances...’s lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000 times (over 800...the broad usefulness of pMD2.G and VSV-G. This plasmid has been used in studies of the secretome, ubiquitin...to check out the Trono Lab website. If you have plasmids you’d like to deposit with Addgene, be sure to...Who knows, maybe yours will be the top requested plasmid of 2018! Additional Resources Quick Guide to ...