We narrowed to 855 results for: NTS

...https://doi.org/10.1126/science.1138389 Le TTH, Tran NT, Dao TML, Nguyen DD, Do HD, Ha TL, Kühn R, Nguyen... veteran and new Rett researchers can find the reagents and resources they need to cure Rett Syndrome....cardio-respiratory dysfunction, distinctive hand movements, and show autistic-like behaviors.  The role of...

...various mechanisms. The RNA guide is typically 20 nts and the genomic locus must often be proximal to a...off-target events There are several strategies to lower the predicted frequency of off-target events, many ... to ensure off-target events are below a given threshold for certain experiments where they can’t be tolerated...single cell clones to use for multiple downstream experiments. You only successfully generate 1 null clone ...single clone is low, but since all downstream experiments will rely on that clone not having any confounding...rate of 5% is far too high when considering human patients and the diseases associated with an elevated mutation...the way of ‘good enough’. Predicting off-target events As mentioned in the above section, there are gRNA...

...flanking primer binding sites (designed to have 20-30 nt length, ~60 oC annealing temperature, and ~50% GC...are influenced by temporally ordered biochemical events. How do RSMs work? The state of an RSM is defined...interrogate state with qPCR. The re-arrangement of DNA segments in each state of a register enables the design... of a set of chemicals. Specific examples of arrangements that should be able to record all permuted substrings...gene regulation programs. The software has two components - a database of pre-compiled register designs...

...generated from a long hpRNA trigger, only a single ~21-nt interfering RNA is produced using shRNA/amiRNA. Although...possible in plants outside of limited, single cell protoplast analysis. Instead, microscopic plants must be...consuming, as RNAi mutants with a reduced growth phenotype will require additional plants to have enough ...LOF studies, the high gene copy number found in plants makes traditional knockouts unattractive from a... In either pathway, short double-stranded RNA fragments (siRNAs) are produced from long dsRNA via an RNase...interpretation of RNAi. Fortunately, researchers studying plants and other organisms lacking this innate immunity...transcriptionally or post-transcriptionally. In plants, different dicer-like enzymes (DCL) process the...

.... Use lots of plants: Lots of plants produce lots of seeds. I typically use 8-10 plants per construct ...of non-transformants and make finding true transformants more difficult. Don’t leave your plants on selective...and Dinneny, 2013). Preparing plants for transformation Whatever plants you are transforming into will...efficiency will be low. Tips: Use healthy plants: Your plants should show no signs of stress, such as anthocyanin...recover a transformant. Use young plants: If you transform early, your plants will still have plenty of time...PCR and glycerol stocks. Dip/drip plants more than once: Dip your plants, let them recover for a week, and...integration. Selecting Arabidopsis transformants Once your seeds from your T0 plants are dry, it’s time to select...

... and purify the DNA fragments. Finally, you simply incubate these three fragments together along with ... region between fragments also better ensures correct assembly order of the fragments as compared to the...chromosome fragments. CRISPR cutting was used to circumvent the need to PCR amplify the fragments of the ...regions, have no requirements for restriction enzymes, and no scar sequences between fragments. Some of these...and fine tuned many different ways to clone DNA fragments which have provided appealing alternatives to ...are using Gibson assembly to put together DNA fragments. As a fun way to start, I highly recommend watching...allowing easy assembly of multiple linear DNA fragments, but can also be used in basic cloning of an insert...

...Delivering CRISPR components to plant cells Once you have selected the correct CRISPR components for your application...deliver these components to your target cells. Remember, efficient delivery of CRISPR components is essential... editors have been developed for plants and prime editing for plants has been developed for rice by Yiping...used to efficiently deliver CRISPR-Cas9 components into plants. Nanoparticles (carbon nanotubes, Kwak ...the potential of CRISPR for genome engineering in plants is also being realized. There are a variety of ...using genome editing to change the genetic code of plants, including the development of crops with longer...requiring several rounds of selection to isolate plants with the phenotype of interest. Genome engineering...

...Successful editing of transformants then typically occurs in T0 plants (from callus) or T1 plants (Arabidopsis ...to betanin, a member of a class of pigments primarily found in plants in the order Caryophyllales, such... family than the red/purple plant pigments found in all other plants, anthocyanins. Figure 1:...the development of the mutant plants, but the seeds needed for experiments can only be collected from heterozygous...heterozygous individuals. Only 1/4 of the descendants of the heterozygous plants, therefore, are useful for research...with these mutant plants. Using RUBY to simplify the identification of these mutants will speed up my ...Antibiotic selection would only then kill the desired plants! PCR or fluorescent markers are currently used ...

...and recruit students to join their societies. Freshers Fairs tend to be used as welcome events for new students... students that provide ways for students to network, find groups with common interests, and settle into...their upcoming events and intro sessions, and give out useful items and information to students. Freshers ...information events and summer internship information sessions to help undergraduate students with their...develop a range of talks for both students and the general public, career events, and networking meetups. The...answer is probably no, but these fun and informative events are one of the main ways budding young scholars...members, who comprise undergraduates, graduate students, postdocs, faculty members, and the general public...

...companies, and governments on the most effective and efficient ways of delivering healthcare. Clients typically... that clients face imminently. Consulting firms deploy case teams, usually with 3-6 consultants, who work...biotech clients. In this realm, there are a few different kinds of challenges that consultants get called...therapeutics. Consultants often work with such teams to help make decisions about R&D investments in either...diseases they can treat, and diagnostic advancements. Consultants can help these teams develop communication...services that help diabetic patients manage doses and timings of their drug treatments. The process of rising...approach brings to patients, doctors, and insurance providers, and is crucial to how our clients’ products will...