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Addgene

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We narrowed to 599 results for: REV;

Showing: 51 - 60 of 599 results

  1. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ... useful. Step 4: Read and learn from the reviewers Reviewers may take 6-12 months to give feedback on...of the art technologies. Therefore, take time to review the literature and offer educated insight into ... the agency and benefits the field reminds the reviewer why your project is the project to fund. Using...limits) provided in this section will convince the reviewers that the proposal is strong, well-thought out,...your advisor to supply a budget form used in a previously funded grant application to see how funds were...application. Make sure to invest time and consider the reviewer's comments. Whether the proposal is funded or not...for improvement, deliver outsider opinions, and reveal items to include for the next application. Although...

  2. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    Published
    Dec. 20, 2018, 1:15 p.m.
    ...was to be revisited at meetings when sufficient time, additional public fora for data review, and more... Bioethics. On November 25, the MIT Technology Review dropped a bombshell report. A scientist working... were born in a twin live birth several weeks previously. While this claim has yet to be independently...report on the ethics of “Novel techniques for the prevention of mitochondrial DNA disorders.” This document...public views on emerging IVF techniques designed to prevent the transmission of mitochondrial disease;” The...Council began their research into both novel and previously established ethical issues raised around mitochondrial...stop shuffling our feet on necessary actions to review and decide on the future of genome editing technology...

  3. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...guide always gets an expert review, separate from the author, and a second review from someone less familiar...channel just for asking people if they can write or review things and another for asking specific science ...answers from our incredible scientists. Expert reviews on blog posts are done on a case-by-case basis,...case of the antibody guide, primary research and previously written Antibody 101 blog posts were used, as...panel recommendations. A few discussions in the review process led to quick dives back into the literature...sharing repositories. Then we format, do a final review to catch those pesky typos, and hit publish. Making...content is an iterative process. We are constantly reviewing the resources we currently offer to identify gaps...

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...off-target activity, and prevent cytotoxic effects. For a more detailed review, check out our blog post...externally control the location, timing, and reversibility of the genome editing process. Read on to learn...expression while imparting spatiotemporal control, reversibility, and repeatability. The system developed by ...ASCL1 gene could be spatially controlled in a reversible and repeatable manner. With their light-activated... after 30 hr. The system was also shown to be reversible and repeatable when blue light (450nm) was cycled...paCas9-2 system could be spatially controlled and reversibly activated by turning blue light on and off. As...repressor (dCas9). The activity of all variants was reversible and repeatable. Since these initial studies ...

  5. A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression

    Type
    Blog Post
    Published
    Nov. 2, 2021, 1:15 p.m.
    ... sites in the same orientation preventing the transgene from reverting. Figure created with BioRender.... (FSF) sequence prior to an ATG start codon to prevent transcription in cells where the recombinase was...direction of arrows) the inverted transgene is reverted into the sense orientation and one of the unused... diagram showing the mechanism of spontaneous reversion during AAV plasmid amplification. On the left ...cre-recombinase sites lead to greater frequency of reversion and leaky expression. On the right non-homologous...scrambled recombination sights lead to reduced reversion and less off-target expression. Figure created...2020) it was determined that the optimal titer to prevent off-target expression of the sensitive FLEx-split...

  6. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

    Type
    Blog Post
    Published
    Sept. 13, 2018, 1:55 p.m.
    ... and identifying >88% of previously identified essential genes while previous Cas9 and shRNA library screens...and non-essential genes for both pathways had previously been identified (Hart et al, Bassik et al). The...10% false discovery rate and identified 67% of previously identified ricin toxicity genes. The screen also... also discovered several genes previously not associated with ricin regulation, including almost all genes...Results from the screen also reproduced several previously observed characteristics that influence gRNA ...A systematic mammalian genetic interaction map reveals pathways underlying ricin susceptibility." Cell...to conduct genome-wide screens with CRISPR/Cas9 Review the basics of CRISPR with this cheat sheet Read...

  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...structures, and genetic mutations are not easily reversible. Late last year, the Akhmanova Lab published ...This design allows highly localized, rapid and reversible microtubule severing and has very low dark-state...Mahlandt et al., 2023). These Opto-RhoGEFs provide a reversible and non-invasive way to activate a RhoGTPase ...light-induced binding of the plasma membrane. In previous publications, they described tools to control ... fusion protein away from its usual location, preventing it from carrying out its proper function (Figure...with these CIB1-Rabs in other contexts are sure to reveal even more insights. Super-duper lipid editor And...composition of the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically controlled...

  8. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...Cells. Annual Review of Neuroscience. 2022 Jul 8;45:447-469. https://doi.org/10.1146/annurev-neuro-111020... Cells. Annual Review of Neuroscience, 45(1), 447–469. https://doi.org/10.1146/annurev-neuro-111020-100834...The blood-brain-barrier (BBB) exists to prevent substances in the blood, like proteins and viruses, from...interest, while miRNA target sites can be used to prevent expression in cell types not of interest. Systemic...both mouse strains. It is therefore important to review the literature for use of a capsid in your strain...al. 2022. Image created with BioRender.com. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory.../ENEURO.0373-21.2022 Brown, D., Altermatt, M., Dobreva, T., Chen, S., Wang, A., Thomson, M., & Gradinaru...

  9. Plasmid Cloning by PCR

    Type
    Blog Post
    Published
    March 29, 2016, 2:30 p.m.
    ...TAAGCAGAATTCATGTGGCATATCTCGAAGTAC-3'. For the Reverse Primer, the design is similar, but we need to use the reverse complement to get...primer and the downstream restriction site to the reverse primer. Next, we need to examine the DNA sequence...homology to the ORF). We now need to generate the reverse-complement of this sequence so that we can successfully...successfully amplify the ORF. You can generate the reverse-complement using existing software (a quick internet...others). If we put the sequence we chose for our reverse primer (5’-TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-...TGGCATATCTCGAAGTACTGAGCGGCCGCTAAGCA-3’) into this calculator we get a final Reverse Primer sequence of 5’-TGCTTAGCGGCCGCTCAGTACTTCGAGATATGCCA...

  10. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...you. Screen variations Forward and reverse screens Forward and reverse screens involve manipulating or ...manipulation of the population. This is contrary to reverse screens, where there is removal of known factors...genes. This is a perfect example of an unbiased, reverse screen – all known genes are assayed for their ...types of screens are very similar to forward and reverse screens, except that they begin with a uniform ...manipulations which either enhance or suppress the previously defined disease phenotype. These screens employ... same downstream manipulations as a forward or reverse screen (CRISPR, drug, etc.), just with an added...
Showing: 51 - 60 of 599 results