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We narrowed to 513 results for: EMB

Showing: 151 - 180 of 513 results

  1. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ...example, DNA assembly methods, such as Gibson Assembly® and NEBuilder® HiFi DNA Assembly, require nick-selective...Plasmid Cloning Techniques Pushing the Limits of DNA Assembly Plasmid Cloning by PCR Resources on Addgene.org...

  2. 2021 Deck the Lab Winners!

    Type
    Blog Post
    Published
    Jan. 25, 2022, 2:15 p.m.
    ....twitter.com/DWKWaPs0iY— Xi Chen (@XiChen7889) December 10, 2021   2nd Place: The Winter and the Falcon...pic.twitter.com/rDaEjxTNZX— Heng Chooi (@YitHengChooi) December 4, 2021   3rd Place: O! Beaker Tree Created by...pic.twitter.com/WMb97fpUOI— Sam Mugford (@samtmugford) December 3, 2021   Congratulations to all our winners,...

  3. Alex Chavez on MD PhDs, CRISPR, Gene Drives, & More

    Type
    Blog Post
    Published
    Nov. 15, 2016, 3:30 p.m.
    ... extensively with CRISPR, and recently became a member of the Addgene advisory board where he will help...and talk with us.  Alejandro (Alex) Chavez is a member of the Addgene Scientific Advisory Board with a...Ready-to-Use CRISPR Lentivirus Learn about Other Members of the Addgene Leadership Team ...

  4. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...pic.twitter.com/X6QYXteRLT — Nika Heijmans (@nikaheijmans) December 21, 2018 Eppi the red nosed reindeer from @ladiesinlabcoats....co/MYMnXicWqb — Matthew J. Sikora (@mjsikora) December 18, 2018 Fireplace from the Dyer Lab If you’re...pic.twitter.com/bDRqAKrWHz — Dyer Lab (@DyerLab_Emory) December 14, 2018 Thanks to all who participated in ...

  5. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...them. Collectively, the pool of CRISPR software embodies solutions that facilitate most experimental applications...in the table): Basic functions: These functions embody the main goals of the software and should be the...genomes are denoted by organism name and genome assembly version. The names in the table appear as they...organisms and will help you whether you have a full assembly or not. It is an offline tool, but comes with ...

  6. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...meetings include all collaborators, from the lab members who are conducting the experiments to the PIs. ...closely replicates how you’d interact with team members in person, and improves overall communication.  ... informal communication you’d likely have with members of your lab that you see in person, and helps improve...

  7. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ... the pores being opened in the pathogen’s cell membrane (Dunkelberger & Song, 2010). As cells cannot survive... survive when holes are punched in their membranes, the pore'd pathogens quickly lyse and die.  Antibody...providing structure and isotype determination, remember that it can do so much more than that!    References...

  8. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...This grows our relationship with our community members. We also find that, as a nonprofit, we attract ...is strength in diversity and in empowering team members to take initiative and give their best As we continue...Research Listen to Our Interview with Addgene Board Member, Connie Cepko Resources on Addgene.org Learn ...

  9. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...characteristics like pH, redox state, and voltage across membranes. These sensors generally follow the same pattern...an organelle-specific targeting signal or a transmembrane domain, biosensors can be targeted to a particular...endoplasmic reticulum (ER), vacuole or cellular membranes. Examples include blue flame mitochondria-targeted...

  10. New and Upcoming Viral Vectors - Spring 2019

    Type
    Blog Post
    Published
    March 26, 2019, 1:40 p.m.
    ...pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) of this membrane-embedding voltage detector. Find Archon1 and its cre-dependent...GABA sensor (pAAV.hSyn-FLEX.iGABASnFR) has good membrane localization and brightness. The F102G variant...

  11. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...& Biology Society (EMBC), 4322–4325. https://doi.org/10.1109/EMBC44109.2020.9175600 Park, S., Loke, G....who together developed multifunctional fibers, embedded, and validated simultaneously in the brain and...

  12. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...10,000 users per month in November 2014 to ~30,000 users per month in November 2015. We will continue ...providing recorded interviews with Addgene community members. In addition we’ve experienced incredible growth...

  13. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...Tat. Although CPP fusions can translocate a cell membrane, the presence of the CPP may alter protein function...various loxP reporters. In both mouse and human embryonic stem cells, they found very high recombination...neuronal and gut stem cells, dendritic cells, embryonic fibroblasts, glia cells and neurons, and cell ...

  14. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...editing system to correct the cystic fibrosis transmembrane conductor receptor (CFTR) by homologous recombination...of CRISPR/Cas9." Cell Stem Cell. 13, 659-662 (5 December 2013). 8. Gerald Schwank et al. "Functional Repair...Fibrosis Patients." Cell Stem Cell. 13, 653-658 (5 December 2013).    ...

  15. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ... out these questions ahead of time so that lab members have time to prepare their thoughts. Then at the...Set up a brief bi-weekly meeting where all lab members talk no science. No data presentation will appear...remotely in science are endless. Let’s adapt and embrace new ways of working, reconnect as human beings ...

  16. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...differentiation, the Zhang lab transduced human embryonic stem cells with the viral TF library, with each...lentiviral particles and used to transduce human embryonic stem cells. Transduced stem cells were differentiated...this screen successfully differentiated cells resembling all three germ layers (ectoderm, endoderm, and...

  17. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...Institute for Biologically Inspired Engineering and member of the Addgene Advisory board, on the difficulty...expressing Cas9. As John Doench (Broad Institute and member of the Addgene Advisory Board) says: "When you'... running. Both Alex Chavez and John Doench are members of the Addgene Advisory Board. Additional Resources...

  18. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ...everyone gets a chance to talk and less vocal team members still have their voices heard. Here's a template...of the agenda, and assign each one using group members’ initials. If appropriate, email this around after...to help keep you accountable for moving along. Remember, many of the more in-depth discussions can happen...

  19. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...and improving accessibility on our website.  As members of Addgene’s content team, we — Jo, Brook, and ... at the Society for Neuroscience conference in November 2023, we hosted our very first professional development...clear writing Let’s use Figure 1 as our example. Remember, our goal is to use words to describe the image...

  20. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ...viral envelope, which fuses with the target cell’s membrane, allowing the viral particle access into the cell...inside the nucleus, without the need for envelope disassembly. Both lenti and retro viruses can accommodate...of murine leukemia virus DNA depends on mitosis.” EMBO J. 12 (1993) 2099-2108. PubMed PMID: 8491198. PubMed...

  22. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

    Type
    Blog Post
    Published
    July 10, 2014, 7:17 p.m.
    ...and names, swapped leadership, and grown to 25 members.  What is Sample6? Sample6 is an enrichment-free...technologies, effectively communicating the advantages embodied, and driving the enterprise value of Sample6.  ...Read On! Synthetic Biology & the Importance of Assembly Standards Story of a SynBio Startup: RevBio's ...

  24. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ... indirect (secondary) methods. In those cases, remember that a secondary should be used when signal amplification...price point, shipping time, or other factors. Just remember that these are secondary considerations to validation...validated using one or more of the proposed pillars.  Remember, validation does not mean that the antibody is...

  25. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ... grow. We are hiring again and getting ready to embark on new exciting projects. Right now we don’t .... We are making an impact.  {% video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist..._playlist=True, viral_sharing=False, embed_button=False, width='1280', height='720', player_id='7039679868...

  26. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...found at Addgene! Figure 1: Strategy for assembling the barcoded gRNA library pool. (Adapted from...barcoded gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone pAWp12...your experiments. Figure 2: Strategy for assembling barcoded combinatorial gRNA libraries. (Adapted...

  28. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...While this method seems simple, I found it hard to remember to describe all four parts of a STAR answer once...Writing a rough outline of these stories helped me remember them for the interview.  Prepare a list of questions...then thank them once the interview is complete. Remember, your interviewers took time out of their day ...

  29. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...poster readable While we are talking about font, remember this: bigger is better! Your poster should be ...poster more navigable.  Choose your content wisely Remember that less is more! Posters should not look like...easily read. Similarly, be cognizant of audience members that are color-blind; avoiding red and green is...

  30. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...Protein A and B interact, the FP fragments can assemble the full structure of the fluorescent protein,...Split superpositive GFP Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting...behavior of the split FP in your system of interest. Remember that the FP fragments themselves can sometimes...
Showing: 151 - 180 of 513 results