We narrowed to 619 results for: LIS

...intellectual property, it worked away in isolation. It published a paper a year (or less) and limited the exchange...public and to other academic labs. Being unable to publish or attend conferences makes it very challenging...As a Master’s student, she had less pressure to publish and more freedom to attend conferences, but after...occasionally thinking about how we could bypass the establishment hiring system altogether. We had a lot of ideas...restricted to science jobs. Keira’s an optimist, I’m a realist, and together we make a pretty good team. We have... – although we didn’t figure out how we would accomplish the color change until a few months later. RevBio...

...access to a list of posters until the day-of, take a moment to step aside to read through the list at the ... a day in advance. Dress for the venue and professionalism of the conference, and remember to wear comfortable...about the relationship of your work to recently published papers or “hot topics,” so be sure to be well-...strategically use your time. Don’t have access to a list of posters at all? No problem! At the beginning ...traverse the sea of posters If you come prepared with a list of posters of interest, then you’ve already set ...

... can be helpful. Publishing: As a postdoc at AstraZeneca, you are expected to publish, but this can vary...prerequisite “industry experience” that I’ve seen listed as a requirement on some industry job postings....vary depending on which company you choose. If publishing is important to you, make sure that your project... Regeneron, and Genentech, among others, and the list is growing. Some post positions as they come up,... Novartis, have specific web sites for postdoc listings, so the method of posting varies between companies...

... thanks to our guest bloggers Alissa Lance-Byrne and Alex Chavez! Alissa Lance-Byrne is a graduate student...This post was contributed by guest bloggers Alissa Lance-Byrne and Alex Chavez, researchers at the Wyss...gene or introduce a desired genetic alteration. Listen to Our Podcast Interview with Alex Chavez ... induce Cas9 to make edits (AC, WLC, and JQ, unpublished results). An additional benefit of the use of...PMC4265475. Additional Resources on the Addgene Blog Listen to Our Interview with Alex Chavez Learn about Sequencing...

... new accomplishments to add. Obtain copies and translations of documents that are not in English. Create...development, consulting, policy, big data and the list goes on and on. As you think about your career (... to obtain permanent residence. The recently established case Matter of Dhanasar provides the three-prong...qualify, you need to satisfy 3 out of the 10 criteria listed below. An international or national award for ...therapeutics to cure the cancer. Authorship:  Publishing books, journals online and/or in print. Having...demonstrate outstanding achievement Evidence of published material in professional publications written ...

... already answered this question and used it to publish!  Working with AAV vectors in neuroscience Let...performance in our system and yet none of that data was published.    Fig. 1: Testing of three different ... for your system or to successfully replicate published works. Of particular importance to these efforts...build on others’ results is available not through published manuscripts, but through word of mouth or old ...asked your team members or colleagues: How much unpublished AAV data do you have sitting in computers and... O’Connor, D. M., Lutomski, C., Jarrold, M. F., Boulis, N. M., & Donsante, A. (2019). Lot-to-Lot Variation...

...made a table that listed each protein in the class, then for each protein we listed all the studies that...include every piece of information that was ever published. Obviously this isn’t possible. So, define your...people cared about. I also used reviews to get a list of research papers that I needed to read. Once I... page as possible. This could be in the form of lists, streams of consciousness, or anything else. Like.... Curate and present some useful data Typically lists and pictures are the most useful parts of reviews...

...specific subjects in the interview. Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic) ...Harvard Medical School researcher Connie Cepko. Listen to the podcast below to hear all about the research... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer... Plasmid Tools Being Developed in the Cepko Lab Listen to our Interview with Michael Koeris Get Cepko ...

...endogenous homologous recombination enzymes can also accomplish the desired genomic modification. This technique... which results in a merodiploid. This can be accomplished by selecting for an antibiotic resistance marker...counter-selection system relies on the sacB gene product of B. subtilis (Hmelo et al., 2015). The sacB gene product is...binding site (using the online calculator from the Salis group) (Espah Borujeni et al., 2014). Replaced the...scarless modification of bacterial genomes. It can accomplish large deletions, insertions, and even precise..., Amin, Anirudh S. Channarasappa, and Howard M. Salis. "Translation rate is controlled by coupled trade-offs...

...get people to remember you). What better way to accomplish all of these things naturally than to give a ... talk without looking at their slides or notes. Listen to their stories and narratives (see below “Tell...presentation appropriately.  Find out who will be listening.  Are they early, mid, or late career?  Will many... Tell a story We are biologically programmed to listen and remember better when we hear material delivered...should be very simple. The goal is to get the listeners to be thinking “What happens next?”  Describe .... During your practice rounds, encourage your listeners to ask questions and record those that were asked...

... Video Production Specialist and Arthur Guerra who is a Customer Support Specialist. Both of these Addgenies...Hola! Que tal? My name is Brandon Solis, and I am a rising senior studying Sociology and Dance at Skidmore...of challenging what I am used to. Brandon Solis, marketing intern. Something that I struggle ... Brandon Solis...

...which he submitted a phony paper to a list of 304 open access publishers. By the time the magazine went to...world. 4)      The Annals of Internal Medicine published systematic reviews on an agent used to promote... into account. 7)     Science’s John Bohannon published an article describing a sting operation in which... publications, although many don’t necessarily publish in OA journals themselves. A good number of those...

...is busy and has a To-Do list long enough that there is no hope of accomplishing it all. How do we encourage...teams at Addgene will forgo their regular To-Do lists (other than emergency items) and focus on a single... Hack Days, and we’ve seen great success at accomplishing “we-should-really-make-time-for-that” type projects...wonderful tradition for university departments to establish, with the goal of fostering better communication...

...along the way in this ongoing series of blog posts. Listen to our podcast interview with Harvard Medical School...’ – inspire you.” “Also a mentor can give you realistic expectations. (Don't think you can finish that...is instructive (and entertaining) to review this list of bad mentor behaviors. Sadly, it is Yoda who is... concrete goals and helps you reach your goals Listens well and communicates clearly and openly Gives ...like to be validating without being coddling, to listen without being taken advantage of, and to respect...

...CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited in...in BioGRID ORCS to allow facile queries of published data by biomedical researchers and ready access to...identifiers and links provided, in addition to a list of individual screens curated for the publication... approach serves to maintain consistency with published screen interpretations and allows users to query...curators at [email protected] to submit their published data for rapid curation review and upload. In ...

... full picture and listen for hints at the same concerns from multiple people. Listen to what they say,...path and help you pursue any career alternative? Listen to our podcast interview with Harvard Medical School...Ask alumni of the lab. Most labs have websites listing alumi and it is easy to use publication records... end of this blog post. This document includes a list of questions to ask your potential advisors and ...

...seems like another dragon to slay on the way to publish your work in a scientific journal. The peer-review...touchstone of the scientific method, ensuring that published work meets the accepted standards of reporting...read, I pay more attention to details and use a checklist for individual sections of the paper (see figures...authors put in the manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge...sharing whatever she knows with whoever cares to listen. In March 2020 she will start her own research ...

... a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast!  MarathonRT: A highly...transcriptase Article contributed by Will Arnold  Listen to the MarathonRT podcast segment Researchers from... surface Article contributed by Marcy Patrick  Listen to the nanobody toolkit podcast segment Nanobodies...Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment Steven Carr’s lab... C-BERST Article contributed by Klaus Wanisch  Listen to the C-BERST podcast segment Similarly to the...

...   Enzyme-linked immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single...samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked Immunosorbent ...example, cell lysate or media from cultured cells. ELISA can be used to quantitatively measure the amount...in a series of samples, such as Western blots or ELISAs, or for other experiments to study protein-protein...

...professors how they hire scientists for their labs. Tom Ellis selects new hires from a pool of CVs collected from...collaborations. Alternatively, perhaps the lab publishes and presents regularly with labs around the world...you might enjoy working closely with others to accomplish your goals. You must understand your own style...for Scientists". This post also includes a useful list of questions for a candidate to ask lab heads, lab... awhile. Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

...a recent plasmid deposit please sign up here.  Listen to the hot plasmids episode! New, highly efficient...knockdown Article contributed by Alyssa Cecchetelli  Listen to the C. elegans RNAi podcast segment RNA interference... miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo podcast segment A ... outbreaks Article contributed by Izzy Mueller  Listen to the viral detection podcast segment As the ... motifs Article contributed by Michael Lemieux  Listen to the SH3 domain podcast segment The human proteome...

...each listed: An “official” name for the container to be used in cross-team communication A list of “AKA...lexicon. The base lexicon was and continues to be a list of commonly used (and misused) words that clearly...the training frequently. In hindsight, getting a list of frequently used words like this when starting...Happier colleagues. Feeling like people aren’t listening to you or understanding you can be incredibly ...

...plasmid deposit please sign up here. No time to read? Listen to our Hot Plasmids Podcast! TRIM-Away: Targeted...degradation Article contributed by Mary Gearing Listen to the TRIM-Away podcast segment CRISPR and RNAi...nanoscopy Article contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment The first...epigenome Article contributed by Joanne Kamens Listen to the Fire-Cas9 Podcast Segment Jerry Crabtree... in yeast Article contributed by Tyler J. Ford Listen to the Synthetic Yeast Transcription Factors Podcast...

...scientists”  These comments are well correlated with published data. For example, in 2010, less than 15% of postdocs...many notable contributions in this area include publishing the book ‘How Economics Shapes Science’ and contributing... Biology at Brandeis University, has recently published an article arguing against reducing the number...postdocs. Among other involvements, he helped to establish grants through the Sloan foundation for postdoc-related...employ them, the diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the...

...helped me remember them for the interview.  Prepare a list of questions to ask your interviewers You’re interviewing...“questions to ask an interviewer,” you can find lists that have lots of general questions about job responsibilities...before the interview. If you are not provided with a list of who will be interviewing you, ask the hiring ... this information. I found it useful to write my list of questions in a notebook that I took with me to.... This is a chance to highlight your prior accomplishments and demonstrate your science communication ...

...recent plasmid deposit please sign up here.     Listen to the Hot Plasmids episode! Expanding the optogenetics...CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust podcast segment The Won Do Heo...optogenetics Article contributed by Meghan Rego  Listen to the soCoChR podcast segment Optogenetic stimulation...operon Article contributed by Angela Kaczmarczyk  Listen to the synthetic decaffeination operon podcast ...fluorescent proteins for use as FLIM-FRET acceptors  Listen to the FLIM-FRET acceptor podcast segment Article...

... set up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting...originality to your work is to collaborate with specialists in different fields that can provide different...insight into an area - not just working within the established network of your peers. Furthermore, using new...of the work was. Ideally this would be results published in a paper, but positive outcomes can include ...

...Biotechnology Communications Specialist at AsisChem Inc. and Social Media Content Specialist at Thermo Fisher Scientific...landscape facing all scientists, both young and established, as they embark on or continue their careers ...effective advocacy strategies on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor... Programs at Personal Genetics Education Project Lisa Coleman, PhD - Chief Diversity Officer Harvard University...

... physical titer are direct p24 measurements via ELISA and qPCR for viral RNA. Measuring p24 levels in ...as there are several commercially available p24 ELISA kits that can quantify p24 levels in a matter of...drawback of this method, however, is that a p24 ELISA measures all p24 in the sample regardless of whether...alternative to direct p24 measurement. Like a p24 ELISA, this method is relatively quick, providing results...than a day, but tends to be less expensive than an ELISA kit. In this approach, viral RNA is first converted...

...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire one image, thereby significantly ...  Thank you to our guest bloggers, Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor...Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an Associate Professor at the University....janelia.org/lab/keller-lab http://www.physikalischebiologie.de http://www.lavisionbiotec.com/ultramicroscope-overview.html...