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We narrowed to 265 results for: rigi

Showing: 151 - 180 of 265 results
  1. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...you used? Share your tips below! This post was originally written by Jason Niehaus in June 2014 and was...
  2. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...cells. In adenovirus, the ITRs serve as a type of origin of replication to initiate replication of the dsDNA...
  3. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...scientific support staff receives – in fact, the blog originally started as a way to easily store and share in-depth...
  4. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ... clever combination of FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a...
  5. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ... deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference...
  6. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...the orientation of the two fragments. However, rigidity can be introduced through mutation, allowing diabodies...
  7. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...homogenization solution and phenol addition of the original no-kit protocol into one step. After homogenization...
  8. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... through factors (both of endocrine and dietary origin) that are diffused or actively transported from...
  9. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...Ward lab created a tool-kit to expand upon these original tools that allows for both spatiotemporal protein...
  10. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ... something completely different than what was originally intended. Sharing unverified plasmids can lead...
  11. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...contain an ampicillin resistance cassette and an origin of replication, which are not schematized. Sizes...
  12. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...functional relevance following a set hierarchy. MoClo originally developed from Golden Gate Cloning, in which ...
  13. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...our chemogenetics poster! This blog post was originally written by Maya Peters Kostman in May 2020 and...
Showing: 151 - 180 of 265 results