We narrowed to 583 results for: BASE;

...Medical School/Brigham and Women’s Hospital (BWH). Based on her experience co-directing Mentor Circle Programs...-Europe professional collaborations, setting up bases and contacts with Spanish laboratories to start ...key to the success of this program. As we build a base of former mentees, we encourage them to get involved...consulting firm focused on new health care models based on integrative and eco-social health strategy, and...their degree. Students filled in a pre-program, baseline self-assessment survey to determine their expectations...expectations of the program and their baseline skills. Mentors were Spanish scientists (MD, PhD and MD/...defining expectations of the program and their baseline skills and a post-program self-assessment, participants...

... after I arrived, I was looking through a paper-based, affordable, and portable microscope called the ... known as gas vesicles) as acoustic biomolecule-based dyes. This technology is widely applicable as bacterial...

... predict. Slower Dephosphorylation Rates: FRET-based reporters can have slower dephosphorylation rates...occurs in the cytoplasm and/or nucleus, while FRET-based reporters can be used to measure kinase activity...

...difficult, but other tissues like the retina have cell-based therapies underway. Chapter 7: Generating entire...entire lungs using a decellurized scaffold as a base. Darrell hopes that further advancement of this technique...

... you. Traditional restriction enzyme and ligase based cloning is a relatively inexpensive way to get the...a try. This method, developed by Invitrogen and based on lambda phage integration sequences, relies on...inserts a breeze. Among them are some of the homology based methods, like Gibson or SLIC, since one can pool...

...viewed more as a guideline, since they are typically based on the copy number of the empty plasmid backbone...matrix could clog the matrix when performing filter based plasmid prepping. Be sure to stay within the recommended...for each plasmid/strain combination individually, based on determining the cell density or by harvesting...

...two broad categories, prokaryotic or eukaryotic, based on the functional elements they contain. Plasmid...polyadenylation? The role of the terminator, a sequence-based element, is to define the end of a transcriptional...terminators are not usually employed in plasmid-based expression systems, so these will not be detailed...

... that need them. Adeno-associated virus (AAV) – based delivery techniques may hold the key. In the simplest...effective. Challenges for AAV gene therapy Although AAV-based gene therapies are promising, scientists are working...should be taken into account when developing AAV-based therapies. Furthermore, deeper understanding of ...

...Francois St-Pierre’s lab developed a new form of FACS-based cell screening, Single-cell Phenotypic Observation...Plasmids and Resources page.  CDDR (CRISPR–Cas9-based Dual-fluorescent DSB Repair) is a double-strand ...double-strand break repair assay for mammalian cells. It's based on the introduction & resolution of two DSBs in a...eliminate competing mobile genetic elements. Image by Basem Al-Shayeb in the Doudna Lab. Scientists looking...

...in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature” library — with some... the deposited scientist  The backbone elements based on the information provided and vector type  Any...assembly issues that arise due to the short 200–250 base pair reads sequenced on the Illumina MiSeq.  Resolving...

...Cas9  NG  - SpRY nuclease  C to T Base Editor SpRY A to G Base Editor SpRY   NYN/NRN NYN is less ...SpRY was validated in double stranded cleavage and base editing applications, with high editing capacity...

...have proposed developing new cloning techniques based on these vectors (Shareck et al., 2004). Plasmid...al., 2023). In this post, we’ve grouped plasmids based on the phenotype they offer their host cell, but...Lee, B., & Miguez, C. B. (2004). Cloning Vectors Based on Cryptic Plasmids Isolated from Lactic Acid Bacteria...

...introduce insertions, deletions, and all possible base-to-base changes.   1. Plasmids 101: Transformation,...

...know it’s there? For this task, you can use PCR based detection, DNA staining, fluorescent tagging, agar...and cost-effective (Sung, 2006). The sensor cell-based method uses specialized cells that can detect the...from different biotech companies which are enzyme-based mycoplasma detection kits. These kits detect mycoplasma...YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species. J Microbiol .44...

...experiences with the new method and look for advice/tips. Based on the discussions in the forums, it appeared that...majority of the respondents (70%) used a lipofectamine-based method to deliver NgAgo ingredients into the cell.... Overall, the real take-home message thus far, based on survey respondents "Final Verdict", is that more...

...lab developed a polymerase chain reaction (PCR)-based system that allows users to generate a plasmid containing...Masato Kanemaki's lab has developed a CRISPR/Cas-based system for tagging endogenous proteins with an auxin-inducible...Schmid-Burgk, Jonathan L., et al. “CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent...

... in this blog post, we're going to focus on PCR-based applications (qPCR and ddPCR.) qPCR Quantitative.... Quantitative analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc Hum Genet...genetic elements can be determined by SYBR-Green-based quantitative real-time PCR. J Microbiol Methods....sample with known copy number set as the expression baseline. Copy number is then determined by comparing results...

..., as it only changes one gene at a time. CRISPR-based systems enable gene knockout in immune cells in ...out a single gene in defined cell types (lineage-based) pXPR_070 Violet-excited GFP (vex) Lineage-Cre...

...in order to increase the accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR...demonstrated the feasibility and power of CRISPR-based library screens in cell lines from humans and other...? The Open Repository of CRISPR Screens (ORCS) database is a new freely available resource developed by...which can also be used to search the entire CRISPR database for particular screens of interest.   Figure...screen data included in the BioGRID ORCS CRISPR database are encouraged to contact BioGRID curators at ...

... inspired to produce intrachromosomal deletions based on the experiments of Kim and colleagues using zinc.... Regulatory DNA function is typically inferred based on correlative biochemical characteristics and ectopic...

...dedicated to generating new tools to manipulate biology based on molecular features found in nature, with the ...recently introduced the SpyTag/SpyCatcher system, based on a protein isolated from Streptococcus pyogenes...

... the very beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing...increase the expression of a gene that is millions of base pairs away. (For details on DNA compaction and the...parsed for spCas9 PAM sites (NGG) and then filtered based on two main criteria: no TTTTT allowed (this is ...here with permission. Quilt is a collaborative database for genomics. In this article, Quilt CTO Aneesh...is CTO at Quilt Data. Quilt is a collaborative database. Aneesh's research interests span machine learning...

..., technical support is often covered by the lab based team as part of their general job duties. Bigger...etc. Technical support roles are primarily office based, and email/phone are the most popular ways to communicate... pressure which are often found in sales or lab-based roles in industry. What to look forward to in a ...answers for customers, internal resources and databases are scrutinized, and this information is enriched...

...would represent a major step forward for CRISPR-based RNA editing - and further experiments strongly supported...CRISPR could cut DNA, the second generation could do base editing, and with the third generation came Prime...ability to now make any small changes, including base edits, insertions, and deletions. But CRISPR’s uses...deletions and small insertions, leaving a several kilobase-sized gap in the gene editing field. The AbuGoot...now developed PASTE, which can “drag and drop” kilobases’ worth of DNA with CRISPR-driven precision into...

...label based on antibody-like sensors) changes that. Developed by the Arnold Lab, ATLAS is based on an ...synapses, and cytoskeleton components) and in a FRET-based biosensor for cAMP dynamics. The mGold2 variants...

...the most popular options, so most of this post is based on reading results from a NanoDrop. The principles...calculate a sample’s nucleic acid concentration based on the sample’s absorption at 260 nm (A260). ...use a complementary assay, such as a fluorescence based assay or running an agarose gel. In the case of ...

...number of ways, from small edits that change single base pairs, to chromosomal inversions and large deletions...1 CRISPR systems make up the majority of CRISPR-based immune systems in bacteria and archaea. Class 1 ...deletions of up to hundreds or even thousands of base pairs, stopping either at boundaries defined by ...originally suspected that Cas3 moved a couple of kilobases down the strand of DNA, Cascade caught up to it...

...common to use many preys with a single bait in Y2H-based screens. In this way, proteins that interact with...proteome of a particular cell type, or a subset based on a cellular compartment or growth stage). A prey...protein-protein interactions in mammalian cells based on protein splicing. Anal Chem. 2001; 73:2516-2521... Including a prey-only control also provides a baseline level of reporter activation. Vary the expression...

...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...Arbabi-Ghahroudi et al., 2017). Although they’re based on the immune system of llamas, synthetic nanobodies...mixture of proteins. The proteins are separated based on molecular weight by SDS-PAGE, and are subsequently...well as tools for developing and producing plasmid-based antibodies - and we’re always growing our collection...

...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...

...lab at Duke University developed similar systems based on the light-inducible heterodimerizing cryptochrome...systems have been developed for use with CRISPR-based gene editing (see table below). For example, the...possibilities for optogenetic manipulation of CRISPR-based systems in animal models. Use Anti-CRISPRs to Turn...Hasegawa S, Yoshimoto K, Sato M (2017) CRISPR–Cas9-based photoactivatable transcription systems to induce..., Kawano F, Suzuki H, Sato M (2015) CRISPR-Cas9-based Photoactivatable Transcription System. Chemistry...transcription Zhou et al., 2020   NPOM-caged nucleobases Cas9, photocaged-gRNA Gene editing For more...

... Santalucía Lab has developed a cloning toolkit based on the Multisite Gateway® technology from Life Technologies...MultiSite Gateway Kit is a customizable Gateway®-based toolkit that allows scientists to generate fusion... Medical School has recently developed a CRISPR-based multicolor tool enabling the imaging of multiple...cancer-associated chromosome shattering. This method is based on the recognition of genomic loci by three orthogonal...does it work? RUSH is an ingenious two-state assay based on the reversible interaction between a hook protein...Neveu laboratory designed a new iterative, chaining-based cloning method that simplifies the process of constructing...presented Archaerhodopsin 3 (Arch) as a new rhodopsin based voltage indicator for use in the detection of neuronal...

...to provide improvements to existing technologies based on polymerase processivity. Please find the MarathonRT...Myers, a Scientist in the group, developed a CRISPR-based approach to detect proteins that associate with ...

... time, we’re developing ourselves as a knowledge base for the processes of viral production and testing...connections. Expanding the viral vector knowledge base: You can help! We’ve been expanding our viral vector...

...tools to overcome this restraint include an AAV-based sparse labeling system where a limiting amount of... truncated attP site. The three SPARC variants, based on the length of the attP site, allow for dense ...

... 2015). All of Addgene’s antibodies are plasmid-based and recombinant. Learn more about recombinant antibodies...affinity reagents, such as nanobodies and ScFvs, based on community demand. Figure 1: Antibody...from Addgene via our Antibody Data Hub once the database is available. This data will help the collection...

...Addgene’s collection also includes a base editing sensor library, a base editing knockout library, a prime...

...alternative careers. Science Careers has developed a web-based, career-planning platform called the Individual ...to learn about a variety of science professions based on their skills and interests. Once scientists identify...

...found in my tissue.” It is based on the same principles as other antibody-based assays, like an ELISA or...cases, a whole organism. But, as with any antibody-based technique, validation and proper experimental design...different companies, such as Validated Antibody Database and CiteAb. The antibody you choose can make or...

...should be in real scientific practice, so it is based on a win-win strategy. After each team presented...developed to teach gamma-retrovirus or lentivirus-based ex-vivo gene therapy’ strategies to undergraduates...