.... On this page, you can also find a table with a list of methods and the species they were used in. iPSCs...successfully differentiated from human iPSCs. Below is a list of some of these cell types [13]:  Germ cells...approaches differ in several ways. Below is a table listing some of critical differences (adapted from Zhou...Complete removal to convert to iPSCs and re-establishment after Partial rearrangement from one cell type... pursue a career in science communication and publishing after completing her postdoctoral training from...

...conformation changes. Several types of assays, such as ELISAs and Westerns, frequently denature the proteins ...absorbing of the proteins to the microtiter walls for ELISAs and a more accurate separation of proteins by mass...Monoclonal antibodies suitable for a Western or ELISAs may therefore often be specific to denatured versions...validated for Western blots, but find one validated for ELISAs, it has a good chance of also working for a Western...

...blogger Courtney Price, the Education & Outreach Specialist for the Arabidopsis Biological Resource Center...Plant Sciences at The Ohio State University. Established in 1991, the Arabidopsis Biological Resource ...Center (ABRC) set out to remedy that with the establishment of an outreach program in 2011. More than a...!  Courtney Price is the Education & Outreach Specialist for the Arabidopsis Biological Resource Center...

...Mentee: Make a list of topics/skills you are interested in exploring. Mentor:  Make a list of topics/skills...discuss these in person. See the extensive resource list available from this post. Use an activity to start...the discussion after that will lead to learning. Listen to our podcast interview with Harvard Medical School...

... below lists potential EAs and which professional skill sets are strengthened. This short list highlights...innovative solution with a strong impact, or a journalist does not understand the high-level science discussed...as the Individual Development Plan to generate a list of potential professions Google potential careers...

... working towards a specific goal, and when you realise that your goal might not lead to a career you actually...available to those who are scientifically minded! Listen to Biotech Founder Michael Koeris' Journey from...really show that you have some of the abilities listed above. Showing that you are willing to exercise...Experiment with New Careers while in Grad School Listen to Biotech Founder Michael Koeris' Journey from...

...Commemorative Travel Awards: Melissa Chiasson, Elizabeth Unger, and Michal Bernach. Melissa Chiasson: Quantifying...one at a time is time consuming and unscalable.  Melissa Chiasson, a graduate student in Douglas Fowler'...Pantoea agglomerans interaction. But the shape and metabolism of leaves can be changed by light and temperature...

...each plasmid item. Given a specific Addgene ID # listed in a publication, you can quickly pull up the associated...Additionally, proper citation of these Addgene IDs in published work allows readers to quickly track down the ...Resource Identifiers (CURIEs) combined with well-established prefix resolution services such as Identifiers.org...that the “How to cite this plasmid” section now lists the multiple PIDs separated by semi-colons. For ...

...long run.  If you are overwhelmed with your to-do list and your team doesn’t seem to have enough to do…...the entire process is completed more efficiently. Listen to our podcast interview with Harvard Medical School...desired outcomes. Concern yourself with what is accomplished, rather than detailing how the work should be...in more engaged, successful employees who can accomplish great things.   To read more on this topic and...

...Outreach Manager for Science Borealis (hyperlink http://scienceborealis.ca/)    ... grant application? Are you familiar with the publishing or patent application processes? How about facilitating...look at the jobs you are interested in. Consider listing the skills you already have, and the skills you...

...genes across the genome. Although this tool was published in 2011, it was only recently made available through...outer mitochondrial membrane. Sequestration is accomplished by knocking down your endogenous protein of ...shRNA. This system requires less work than using established methods and minimizes some of the technical issues...the CRUSH vector in transgenic mice, the lab established a dual-color RNAi “sensor” mouse strain in which...Chen and colleagues for genome editing inXenopus tropicalis. (Guo et al., Development. 2014..) A new, higher...plasmids you would like to seen on our Hot Plasmid list. Other popular plasmids & blog posts @Addgene: ...

...Tools to Try  Checklists Found in surgical suites, air fields, and military bases, checklists are the ultimate...accurately. Thankfully, you should already have a checklist for everything you do in the form of a protocol...protocol a few times, you no longer need the full checklist. Great! But don’t just stop using it cold turkey...

... includes biochemical properties, previously established uses, and intended applications. Within CasPEDIA...containing that enzyme, and embedded in the References lists on every page you can find direct links to plasmids...content and much, much more.     Fig. 2 – List of resources and properties for a Cas entry (SpyCas9a...      CRISPRpedia IGI’s digital resources in English/Spanish  ...

...take a course through the school of journalism on science journalism.” She also interned two days a week...Susan  finds important for her work include: “Listening. Evaluating evidence. Critical thinking. Being...Addgene Blog Volunteer to write for the Addgene blog Listen to Our Podcast Episode on Science Communication...

... vice versa, let’s take a look at cancer cell metabolism. Cancer cells promote oxidative stress leading...of cells affect tumor progression, enzymes and metabolism reprogramming aren’t the whole story. It’s essential...Portugal. She is particularly interested in science journalism and writing about biomedical issues. Follow her...260. PubMed PMID: 16807804. Hanahan, Douglas, and Lisa M. Coussens. "Accessories to the crime: functions...

... for more offerings. The viral vector team is publishing research, hosting conferences, and are expanding...documentaries in 3 languages (Japanese, German, and English) and even on 60 Minutes. We are blessed with a ...often focus on the excitement of what we are accomplishing and what more we might do. I am grateful every...embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button=False...

...central and peripheral organs because of their established roles as molecular sensors and governors of endocrine-hormone...gene expression (see www.nursa.org for a curated list of coregulators). Coregulators can function as coactivators... regulatory regime (such as genes involved in metabolism, growth or cytokine action). Primary or core ...the NURSA website will feature direct links to listings of available plasmids for our curated NRs and ...

...dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different genes cloned into plasmids...designated as DE3. For Phagocata morgani and Phagocata gracillis, the process is a little more complicated. These...the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the dsRNA from bacteria and..., is available from Addgene. If you use these published protocols and want to share pictures, feedback...

...disparities. But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists...misconduct. Past ethical breaches such as the Tuskegee syphilis experiment, the case of Henrietta Lacks, the Havasupai...between these communities and the biomedical establishment. This, along with concerns about the use of ...obtaining my PhD and working in digital academic publishing for a year, I’ve since returned to pgEd as a ...

...team from China’s Ribobio, the illustrious speaker list attracted a host of sponsors based all over the ...one take dried seahorse for?), but there were no English speakers around. Opening talks – Genomics discoveries...they are making fairly remarkable progress on establishing a system for the chemical replication of RNA...resistant bacteria. See the picture to the left for his list of the bacterial species that “ESKAPE” antibiotic...

...well defined peptides. Munro and Pelham’s first published peptide tagging experiment involved a peptide ...recognized by Myc1-9E10 down to 10 amino acids (EQKLISEEDL) - larger than the substance P peptide, but still... antibody binds. For example, Myc1-9E10 binds EQKLISEEDL (pronounced “equi-kli-seed-le”). (3) Tag all ...limitations was described by Schüchner, et al., who published a paper showing that the amino acids neighboring...

...enrichment via enzyme-linked immunosorbent assay (ELISA) The enriched sybody pools from the phage display...the protein of interest. The Seeger lab used the ELISA technique described in their protocol because it...starts out with anti-Myc antibodies bound to an ELISA plate to capture Myc-tagged sybodies. Then, the ...detected using a colorimetric reaction. After the ELISA step, the narrowed-down pool (usually 10-30) of ...

... is a list of poster titles or abstracts available before the conference, comb through that list and note... this is being used, it’s best to turn to an established social media platform. During the conference ...something on social media. If someone is presenting unpublished data they may ask the audience to not share their...

...’ button a new content-aware suggestion dropdown list will let you jump directly to the type of information...to the plasmid page at the bottom of the dropdown list: The search bar will suggest direct links to other... antibody plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94...the ‘X’ next to any filter to clear it. Here’s a list of available filters to refine your search results...

...specific cell types (Rouet et al. 2018). This was accomplished using a Cas9 mutant (Cas9M1C/C80S) that harbors...been an area of intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection... transfection of protoplasts and biolistic bombardment of immature embryos (Liang et al. 2017).  PEG-mediated... to perform, often show low efficiency, while biolistic bombardment, which can have high efficiency, requires...

... Protein tags are usually smallish peptides incorporated into a translated protein. As depicted in the...purification with amylose, but wicked huge Myc EQKLISEEDL  1.2 Affinity Frequently used for western blots...tags and fusion proteins as examples. The tables list more common tags than are described in the post,...Journal 2012 , (7): 620-634. Full Text A complete list of free handbooks for protein purification is provided...

...fluorophores are constantly being engineered. Table 1 lists a few common fluorescent proteins and their mutations...mutations relative to wildtype GFP. Although not listed here, many permutations within each color also exist...plasmids as a means to many functional ends. We’ve listed our favorites below, but many other uses currently...Haimovich of greenfluorescentblog. Gal will share his list of 10 things to keep in mind when choosing the best...

...reversible. Late last year, the Akhmanova Lab published a new optogenetic tool for localized microtubule...transport, and cell division, but given the long list of roles played by microtubules, Opto-katanin will...understand their roles in forming the cilia that help establish left-right organization of the embryo (Aljiboury...the membrane itself? The Baskin Lab previously published OptoPLD, an optogenetically controlled phospholipase...

... contest is now closed. Stay tuned to see the finalists and vote for your favorite! Our mascot who has...neutral name. The Addgenies will choose up to four finalists and we’ll ask you to vote for your favorite name...We hope you submit your best ideas because the finalists will receive some newly designed Addgene swag....

...Mammalian Cells Note: This is not an extensive list Acceptor labeling: Sometimes it's okay to be wrong...However, proper control experiments are needed to establish the dynamic range of your assay. These experiments...energy transfer values you can expect, thereby establishing the dynamic range of your FRET assay (Figure... developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies. FRET experimental...

...Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist, Rebecca. They will be working...is rounded out with our science communication specialist (you'll know me by my Tweeting — er, posting!...

...helped us update many of our educational resources. Listen to hear all about Professor Goedhart, his lab, ..., and some of the tools he’s developed.   Listen to the interview with Joachim Goedhart Podcast breakdown... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...learn how they think about communicating science. Listen to the interview with Science in the News, Part...Non-Scientists How to Approach Different Audiences Listen to the SITN Podcast Many thanks to our guests,... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed...This selection should populate your “PubMed filter list” to the left of your screen. That’s it! Next time...to set-up emails to alert you when papers are published on your favorite topic. If you have any other...

...board where he will help shape Addgene's future. Listen to this episode for insights into the process of... tools, and the potential uses of gene drives. Listen here! Podcast breakdown (Time : Topic) 0:00 - 2... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...some time learning and doing research at Novartis. Listen to learn how Kwabena plans to use his research ...opportunities to Ghanaian scientists Click here to listen! Podcast breakdown (Time : Topic) 0:00 - 7:11 :... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...great advice on all of these topics. Listen to Part 1 Here Listen to Part 2 here! Time : Topic 0:00 - ... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

... on sustainable practices in the lab, where I’ll list some easy but effective habits you can incorporate...long-term goals to stay motivated and to feel accomplished. In addition to doing some of the quick things...has a lot of resources about this, and even has a list of biological samples that are stored long term ...

...transition from graduate student to entrepreneur, listen to our full interview with Dr. Koeris below. Check... specific subjects in the interview and happy listening! Download the podcast here! Podcast breakdown ... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...simple as possible. We are the Customer Support Specialists here at Addgene. If you’ve called our customer... purchase order, which is an official document listing materials and pricing information that is sent ...send an invoice to the billing address that you listed during checkout. This invoice will reference your...