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Using CRISPR/Cas9 to Edit Disease Out of the Genome

Type

Blog Post

Published

June 23, 2022, 1:15 p.m.

...’t yet been realized. The researchers chose to explore its potential in mice with a dominant cataract ...
Trading Spaces - Visiting Scientist Edition

Type

Blog Post

Published

Aug. 22, 2023, 1 p.m.

...you. Here, we will chat about who might want to explore this role, how to seek out such an arrangement,...
Multiplexed Overexpression of Regulatory Factors (MORF) collection

Type

Blog Post

Published

March 30, 2023, 1:15 p.m.

...community clusters of individual cells along with color coded nominated reference cell types. (Adapted ...
Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

Type

Blog Post

Published

Dec. 10, 2019, 1:58 p.m.

...apoB gene. Using this reporter, the researchers explore many aspects of ApoB-LP biology, including quantification...
Plasmids 101: NGS Quality Control for Pooled Libraries

Type

Blog Post

Published

Oct. 26, 2017, 1:59 p.m.

...create a “cumulative wealth distribution” curve, or Lorenz curve. By calculating the area under the curve ...
Four Factors that Differentiate the Stem Cell Field

Type

Blog Post

Published

Sept. 28, 2016, 2:35 p.m.

...Shinya Yamanaka, George Q. Daley, Christine Mummery, Lorenz Studer, and Deepak Srivastava--addressed a battery...
5 Great Apps for Lab Life

Type

Blog Post

Published

Sept. 22, 2016, 2:30 p.m.

...reading this article has a smartphone nearby. We tailor these devices to our own interests with the content...
R Bodies: Membrane-Rupturing Microscopic Tools

Type

Blog Post

Published

April 14, 2016, 2:30 p.m.

...their natural function (see below). Or you could explore their ability to penetrate other barriers (like...
Resume Writing for Non-academic Science Careers

Type

Blog Post

Published

Dec. 18, 2014, 4:06 p.m.

... what should be highlighted on your resume, and tailor the resume for the job posting. For example, if...
Fluorescent Proteins 101: Luciferase

Type

Blog Post

Published

June 24, 2014, 3:59 p.m.

...complex cell treatments can be designed to further explore the pathway. Which luciferase and assay have you...
Improved Plasmid Maps Powered by SnapGene

Type

Blog Post

Published

June 29, 2017, 1:06 p.m.

...table includes feature names, locations, sizes, colors used to indicate them on the map, direction (if...
A Practical Approach to Choosing the B(right)est Fluorescent Protein

Type

Blog Post

Published

June 1, 2017, 2:30 p.m.

...I Use? Choosing Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors...
Addgene’s Viral Service - Why Virus? Why Now?

Type

Blog Post

Published

March 23, 2017, 2:30 p.m.

...nonprofit best practices and encouraging us to explore new directions. I would like to thank them for ...
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

Type

Blog Post

Published

May 3, 2018, 1:48 p.m.

...favors cleavage at uracils, but a more detailed exploration of this cleavage pattern is necessary. Konermann...
Early Career Researcher Toolbox: Social Media for Scientists

Type

Blog Post

Published

Dec. 11, 2018, 12:46 p.m.

... wrote a blog post about a research paper that explored how scientists connect on Twitter that I realized...
AAV Q&A with Tim Miles

Type

Blog Post

Published

May 17, 2022, 1:15 p.m.

...This will be highly dependent on vector dose. The color diversity in Vector Assisted Spectral Tracing (VAST...
Flow Cytometry Readouts: Yes, No, and Everything in Between

Type

Blog Post

Published

Aug. 20, 2024, 1:15 p.m.

...you’ve also adjusted voltage and compensated your colors. Here are some good practice tips on adjusting...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

Published

Aug. 6, 2024, 1:15 p.m.

... References and resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses...
Viral Vectors 101: Biosensors

Type

Blog Post

Published

July 6, 2023, 1 p.m.

... fluorescent output, which will turn on, change color, or change intensity of fluorescence in the presence...
Viral Vectors 101: Viral Applications

Type

Blog Post

Published

April 13, 2023, 1:15 p.m.

...etc.) to raise immunity. Viral vectors are being explored as the delivery mechanism for increasingly popular...
Sharing is Caring: But How to Distribute Open Hardware?

Type

Blog Post

Published

Feb. 28, 2023, 2:15 p.m.

...cutting-edge research to everybody by relying on tailored image processing and low-cost optical setups. ...
Clear and Accessible Writing

Type

Blog Post

Published

Feb. 15, 2024, 2:15 p.m.

...workshop with you! Accessibility In order to explore accessibility, we first have to define it. There...
Behind the scenes: Addgene’s new search engine and more

Type

Blog Post

Published

Dec. 12, 2019, 2:22 p.m.

...our user experience experts, our software team tailored Elasticsearch to support the different usage patterns...
7 Tips to Secure a STEAM Internship This Summer

Type

Blog Post

Published

Feb. 2, 2021, 2:15 p.m.

...areas! 2. Resume and cover letter know-how Tailor your resume to the audience that will be reading...
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Type

Blog Post

Published

July 10, 2014, 7:17 p.m.

...Concisely and effectively presenting the value tailored to each and every audience uniquely (not a focus...
Plasmids 101: Yeast Vectors

Type

Blog Post

Published

Feb. 25, 2014, 7:11 p.m.

...methylmercury. S. cerevisiae no no Can be used for color and growth selection if divalent lead ions are used...
Lighting Up Cell Signaling with Photoswitchable Kinases

Type

Blog Post

Published

July 25, 2017, 1:20 p.m.

...goes dark, and MEK1 is active or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when ...
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

Type

Blog Post

Published

June 15, 2017, 2:30 p.m.

...I Use? Choosing Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors...
Choosing the B(right)est Fluorescent Protein: Photostability

Type

Blog Post

Published

June 8, 2017, 2:30 p.m.

...I Use? Choosing Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors...
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

Type

Blog Post

Published

Feb. 2, 2018, 3:20 p.m.

...Communication Posts Resources on Addgene.org Explore careers at Addgene Other Resources The National...
Celebrating 15 Years of Scientific Sharing

Type

Blog Post

Published

Jan. 14, 2019, 2:08 p.m.

...organizational level, we keep passing our audits with flying colors. While of course always being vigilant about resources...
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

Type

Blog Post

Published

March 3, 2020, 2:15 p.m.

...recombine independently and results in a variety of colors depending on the combination of fluorescent proteins...
Antibodies 101: Affinity Reagents

Type

Blog Post

Published

April 14, 2022, 1:15 p.m.

...either with or as fluorophores in a wide variety of colors. To antibody or not to antibody? That is the ...
The time and cost required to make a plasmid

Type

Blog Post

Published

March 23, 2023, 1:15 p.m.

...money or time, there are a few options you can explore to maximize the use of both when making plasmids...
Giving gRNAs a Facelift - Synthetic and Beyond

Type

Blog Post

Published

Oct. 20, 2022, 1:15 p.m.

...modifications are even going beyond stability, adding color and even on-off switches. Stabilizing gRNA modifications...
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

Type

Blog Post

Published

April 12, 2016, 2:30 p.m.

...any genetic elements of interest. It can thus be tailored to address the users’ specific research questions...
Addgene's Tips for Plasmid Quality Control

Type

Blog Post

Published

Jan. 14, 2016, 3:30 p.m.

... Julian Taylor-Parker...
The Future of Research Symposium Boston 2015

Type

Blog Post

Published

Oct. 20, 2015, 2:30 p.m.

...fields (6). Melanie Sinche is a STEM career counselor, Director of Education for the Jackson Laboratory...
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

Type

Blog Post

Published

March 8, 2017, 3:15 p.m.

... apramycin, blasticidin, bleocin (zeocin), chloramphenicol, erythromicin, gentamicin, hygromycin, kanamycin...
Pathways Over Time Plasmids Engage Students in Functional Genomics Research

Type

Blog Post

Published

May 22, 2018, 1:38 p.m.

...about Basic Lab Techniques High School Students Explore Careers at Addgene Resources on Addgene.org Find...

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