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We narrowed to 573 results for: BASE

Showing: 251 - 300 of 573 results

  2. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...conduct their hands-on experiments.  As a project-based curriculum, BioBuilder expands learning outcomes...Mary Tamer from BioBuilder! Mary Tamer is a Boston-based writer, editor, and consultant whose work is focused...

  3. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...commonly used for the prediction of T cell epitopes based on the amino acid sequence of the antibody.  NetMHCPan...Assays to Assess Immunogenicity Risk of Antibody-Based Biotherapeutics. PloS one, 11(8), e0159328. https...

  4. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...to altered function or maybe your favorite FRET-based biosensing technique can be applied to an important...experiments enabled by physical, software, and biology-based tools as well as collaborations between different...

  5. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...scientists can still reap the benefits of plasmid-based research using synthetic vectors and cultured mammalian..." mammalian ORIs, scientists have usurped viral-based ORIs to fill the void. These ORIs, however, require...

  6. Meet Aavery, Blugene's New Virus Friend!

    Type
    Blog Post
    Published
    Feb. 13, 2020, 2:34 p.m.
    ... more than one person, we selected the finalist based on the first time of submission.)     We love...

  8. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...staining. Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...of two. There are four IgG subclasses, numbered based on their abundance in blood: IgG1, IgG2, IgG3, and...

  9. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...by gel or by nanodrop to assemble the  fragments based on their molar ratio. Assembly of the fragments ...you can assemble the different parts of a plasmid based on the cloning strategy you followed. While most...

  10. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...critiques or endorsements. In contrast, journal-based peer review is usually a confidential process among...ensure a manuscript’s quality. But even journal-based peer review can be prone to biases or errors, with...

  11. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...all be ssDNA within the viral particle. The ITRs base pair with each other to allow DNA synthesis of the...57 human adenovirus types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral...

  12. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...mechanism for increasingly popular nucleic acid-based vaccines. These vectors provide several advantages...delivery system for human patients. Multiple viral-based gene therapies have already been approved by the...

  13. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...hours of hands-on time to make a single plasmid, based on the assumptions outlined in Table 1.   Of course...performing the experiments. This cost varies hugely based on a number of factors, but we can assume it’s at...

  15. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...that covers most things CRISPR - from nickases to base editing to pooled library screening. It also has...

  16. Lucky Thirteen: Guest Contributors of 2023

    Type
    Blog Post
    Published
    Dec. 5, 2023, 2:15 p.m.
    ...Open Hardware? Author: Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author...AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers...YanevCellosaurus: A Cell Line Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic ...

  17. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...causes of irreproducibility with some solutions based on what I’ve learned. I hope you can apply some ...all human extracellular proteins with community-based validation. They also have plans to expand to other...with larger and larger datasets and use computer based analysis techniques, an important component of reproducibility...on the Addgene Blog FPbase: A new community-editable fluorescent protein database ReFigure: Save Scientific...technologies later. Organizations such as BenchSci and FPbase are working hard to enable researchers by making...BenchSci collates information about antibodies while FPbase curates information about fluorescent proteins ...

  18. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...TAG. Design your repair template with 55-100 base homology arms upstream and downstream of the insertion...using the ivTRT method, try to make the first two bases of the left homology arm guanine (i.e. GG); this... PMC3961003  Miura, Hiromi, et al. “CRISPR/Cas9-based generation of knockdown mice by intronic insertion...

  19. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...References Abril, U.N.O., et. al. (2022) A plasmid-based system for locating and containing escaped flies...

  20. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...the 3rd day of CRISPR, Addgene gave to me: dCas9-based gene editing for cleavage-free genomic knock-in ...

  21. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...learn how to communicate their work differently based on their audience. How you talk about your experiments...problem was the creation of an Addgene lexicon. The base lexicon was and continues to be a list of commonly...

  22. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...the concentration of proteins in an ELISA well is based on a colorimetric reaction. Most commonly, this ...concentration of protein in the experimental wells based on the equation of the line. With these tips in ...

  23. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a new tool developed... quantification of this depletion using imaging-based measurements (Ashley et al., 2021).   Schematic...

  24. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...the Rare Genomics Institute by providing plasmid-based tools for rare disease research. Congratulations...events, we will utilize BRET-, FRET-, and intensity-based signaling biosensors to monitor intracellular signaling...

  25. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    Published
    Oct. 20, 2022, 1:15 p.m.
    ... modified) for optimal stability and efficacy.  Base modifications What is more stable than RNA? DNA!...gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation. ACS 6, 5, 695-703 (2020). https...

  26. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... broadens the conversation about genetics pgEd, based at the Department of Genetics of Harvard Medical...universities or cities have organizations (often volunteer-based) that put on public lectures, science cafés or science...

  27. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ... delivery systems, especially lentiviral vector-based CRISPR pooled libraries that allow functional screening...barcoding library consists of 96 retroviral plasmids (based on the Murine Stem Cell Virus, MSCV) each with a...

  28. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...protein for live cell imaging would be mTurquoise2, based on the data acquired at low excitation powers. Note...Amsterdam) where she develops genetically encoded FRET based biosensors.     Additional Resources on the Addgene...

  29. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ... on the identification of autophagic structures based on morphology. Autophagosomes are relatively easy...summary of what color fluorescence Rosella emits based on its localization. Table 1: Summary of Rosella...

  30. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...and sensitive enough to detect gene copy number based on the number of fluorescent puncta observed. By...Addgene Blog Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent...

  31. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...barcoded gRNA library is now ready for CombiGEM-based assembly into the CombiGEM lentiviral vector backbone...: PMC4420636. 7. Ma, Hongming, et al. "A CRISPR-based screen identifies genes essential for West-Nile-...

  32. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...expand your search. Results in your feed are ranked based on their influence on the network, or body of literature...library. The library will suggest additional papers based on the papers you have saved. This library can also...

  33. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...Nanoblades are a Cas9 RNP delivery vehicle that’s based on mouse leukemia virus (MLV) virus-like particles...Homology directed repair (HDR) with Nanoblades CRISPR-based HDR allows for the precise insertion of a DNA sequence...

  34. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...specificity. Strength and accuracy The strength is based on binding specificity and exposure time. We first...: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ... dedicated to creating a public and accessible database for low-cost, high-quality antibodies for IHC....reference. The Gift of Giving As an open-source database, the mAb3D Atlas will foster collaboration, progress...reliable, high-quality antibodies. We are creating a database for goldilocks antibodies and, alongside that,...

  35. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...this limitation, we created a generalizable tag-based strategy, which we refer to as the degradation tag...system is versatile and can be used in both cell-based assays and mouse models. The dTAG system The dTAG...

  36. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ... lentiGuide-based) Mammalian Lentivirus pRDA_722 (CMV-driven; lentiCRISPRv2-based; preferred ...

  37. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...mammalian cells. Despite the popularity of CRISPR-based knockout/knock-in systems, these systems remain ...difficult. Addgene depositors have developed new CRISPR-based methods for various knock-ins, discussed in our ...

  38. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...your email or phone call. Though my experience is based solely on my time at Addgene, I’m confident that...minutes from our phone call. If your concern is based on data you have produced, please share the data...

  39. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...illustrious speaker list attracted a host of sponsors based all over the world. With three Nobel Prize winners...hypothesis that these pathways are involved in RNA based surveillance of repetitive gene families and provide...

  40. CRISPR 101: Mammalian Expression Systems and Delivery Methods

    Type
    Blog Post
    Published
    Sept. 24, 2015, 2:30 p.m.
    ...or repressor domains to control gene expression. Base editors can help you make precise point mutations...consider the best form for those CRISPR components based on your model system. CRISPR reagents can be delivered...

  41. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...photos/72005145@N00/5600978712/ What CRISPR-based reagents are available for genome-wide screens? ...enriched using either drug selection or fluorescence-based cell sorting and screened for a particular phenotype...

  44. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...event, and assigns each event to a channel number based on intensity. Higher intensity events are assigned...to the protein in its native state.  Antibodies-based flow cytometry can use both direct and indirect ... background information for setting up antibody based flow cytometry experiments.       References and...

  45. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    Published
    March 21, 2024, 1:15 p.m.
    ...is vital for the accurate interpretation of FRET-based experiments. A popular combination is Alexa594, ...dyes, expanding the capabilities of fluorescence-based techniques in biotechnology. Recent examples of ...2016). Development of a quantitative fluorescence-based ligand-binding assay. Scientific Reports, 6. https...

  46. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...questions, which are critical in setting up a FRET-based assay or screen (the Addgene blog has an excellent...different city, you can still develop a successful FRET-based assay since energy transfer only occurs when donor...10. Saraheimo, Satu, et al. "Time-Resolved FRET-Based Approach for Antibody Detection–A New Serodiagnostic...

  48. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ... John Doench et al. shows that mutating a single base pair may not be enough to prevent re-binding. Lastly...region in a UTR, CrispyCrunch flips the two important base pairs of the PAM (NGG -> NCC, CCN -> GGN). This ... much as possible, it uses standard tools and databases, including primer3, biopython, bwa, samtools, ...

  49. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...point where the hydrogen bonds break between the base pairs. This results in the separation of the two...using PCR. qPCR requires the addition of a probe based fluorescent dye that intercalates with any dsDNA...

  50. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... scanning By Seth Kasowitz As multiple antibody-based vaccines are deployed around the world to combat...deactivate photocleavable gRNAs within seconds. A CRISPR-based lineage tracing tool captures rare clones that represent...
Showing: 251 - 300 of 573 results