Skip to main content
Addgene
Showing: 251 - 300 of 510 results

  1. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...now, it’s time to cast aside those concerns and embrace a new resource to solve the problem: CasPEDIA, ...collection for plasmids containing that enzyme, and embedded in the References lists on every page you can ...

  2. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...rmmAkm6PQd — Elizabeth Kenkel (@ElizabethKenkel) December 5, 2018 How social media has helped me in science... researcher’s work day. It’s also important to remember that it’s not the amount of time you spend on ...

  4. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ...your network, which includes your friends, family members, and professional network such as co-workers, professors...narrative that is positive, and forward-looking. Embrace it, and let your personality leap out of the page...

  5. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    Published
    Nov. 24, 2020, 2:15 p.m.
    ...customers, software developers, as well as team members in automation, sales, marketing, and more! This... up. Finish any communications with my UK team members before they log off for the day. 1:45 PM--Write...

  9. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...you give someone a card they are more likely to remember you if you follow up and if you give someone your... online to follow up with in person. Email lab members that have moved on to see if they will attend. ...

  10. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...use in stem cells. (See the complete protocol at StemBook). Addgene: How are you now using this tool? Musunuru...includes 834 plasmids for the pieces you need to assemble. Our system for CRISPR/Cas9 (also available from...

  11. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...proliferation of basal-like breast cancer cells. eLife. September 2017. 6: e26693. PubMed PMID: 28926338. PubMed...Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588.e28 PubMed PMID: 29224777...

  12. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...between one heavy chain and one light chain. When assembled, the four chains make a Y-shaped protein that ...antib8020028    Wessel GM, McClay DR (1986) Two embryonic, tissue-specific molecules identified by a double-label...

  13. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...and targeting abilities of the other Cas family members make for useful implements in the scientist toolbox...Spectrum of Long-Range Genomic Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular ...

  14. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...going to pour these plates now” to a fellow team member. But, at Addgene, we use seven different types ... professional life, let me know that, too. And remember, if you discover during grad school or during ...

  16. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    .... Protein kinases deliver messages to the next members of their pathways, but where the kinases are located...nucleus. But if the inhibitor targeted upstream members of the pathway (Raf1, EGRF), the KTR localized ...

  17. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    Published
    April 13, 2017, 2:30 p.m.
    ...Autophagosomes are relatively easy to identify: double-membraned structures containing undigested cytoplasmic contents...stands for protein) associates with autophagosomal membranes, so tagging it with GFP allows for tracking the...

  18. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies, as ...resources and protocols! We invite feedback from all members of the research community to help us develop these...

  22. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...targeted DNA modifications in transgenic animals, embryonic stem cells, and/or tissue-specific cell types:...oncogene only in a particular organ, or bypassing embryonic lethality. To facilitate the use of Cre-lox technology...

  23. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...days, weeks, or months. It is always helpful to remember that if a PI asks for assistance in the grant ...of the statements may be slightly far reaching, remember that you are writing a proposal to test hypotheses...

  25. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...TET, can, like the iron ball-and-chain that they resemble, actually impede your investigation into the regulation...CpG so that nothing else can interact with it. Remember, the goal of this experiment is to expose an unmethylated...

  26. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...variants:   Figure 2: Inner mitochondrial membranes imaged with structured illumination microscopy...2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature Methods, 1–9. https...

  27. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ...sensors available that will localize at the plasma membrane. GCaMP-X is a nucleus-targeted GCaMP6 with an ...the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References and Resources...

  28. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...have a half life of 84 hours in primary mouse embryonic fibroblasts. This fusion protein can be used to... The researchers tested several scenarios from embryo development to myeloid proliferation in acute myeloid...

  29. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...will be held on Friday 16th and Saturday 17th September respectively. Join us to discuss advocacy efforts...scientists, both young and established, as they embark on or continue their careers in science-related...

  30. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...overlooked regulatory fragments of these transcripts. I remember when small RNA was just something we ran off the...hypothesized that life started with RNA and a simple cell membrane. His recent work shows that they are making fairly...

  32. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... adaptable into future magnetogenetic tools.  Embracing science in the open The York Lab operates with...applications across a wide range of topics. He embraces open science, self-publishing directly on his ...

  33. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...associate with the endogenously expressed SCF complex members, an E3 ubiquitin ligase complex. Finally, when ...Chem Biol. 2015 Sep;11(9):713-20. doi: 10.1038/nchembio Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi...

  34. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...aliquoting purified and buffer exchanged virus, remember to make one or two small aliquots to use for qPCR...Tips When calculating the titer for your AAV, remember to account for the dilution of the sample for ...

  35. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    Published
    May 23, 2019, 12:26 p.m.
    ...break), write down some notes on the card so you remember who they are and what you’ve talked about. After... Resources on the Addgene website Become of a member of the Addgene depositor community ...

  38. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...development of vertebrate embryos.  The first 3D reconstructions of clear embryos were obtained by using ...

  39. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...else you’d like to say to our readers? Patron: Remember plants. Every time you look at your plate, think...Journal. A modular cloning system for standardized assembly of multigene constructs. Weber E, Engler C, Gruetzner...

  40. Course-Based Undergraduate Research Experiences (CUREs)

    Type
    Blog Post
    Published
    Feb. 9, 2023, 2:15 p.m.
    ...!). In 1997, Joseph Provost, then a new faculty member at Minnesota State (and now Professor of Chemistry...and it can be difficult to give up the control embedded in “cookbook” lab activities (follow the recipe...

  41. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ... derived from the mastadenovirus members (mammalian-infecting members) of the Adenoviridae family, which...

  42. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...2007). Alternatively, if you prefer Golden Gate assembly, there are a series of plasmids from the Dinneny...Arabidopsis.html?id=IfZAMNPWVk4C&redir_esc=y (Accessed: 21 September 2018). Additional resources on the Addgene blog...

  43. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...likely due to quarantining {% video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist..._playlist=True, viral_sharing=False, embed_button=False, autoplay=False, hidden_controls=False, loop=False...

  44. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...want to know the orientation of the insert. One member of this type of primer pair anneals to a sequence...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read about...

  45. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...nucleus. To circumvent this requirement for transmembrane proteins, a split ubiquitin system has been ...genetic system optimized for systematic studies of membrane protein interactions. Proc Natl Acad Sci U S A...

  47. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...technically gamma-retroviruses - another, separate member of the retroviral family. While both lentiviruses...Lentiviruses are able to enter an intact nuclear membrane allowing them to infect both dividing and non-...

  48. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    Published
    March 20, 2014, 3:04 p.m.
    ...Maybe you’ll need to care for an elderly family member, want to train for a triathlon, finally buy that...laundry, take public transportation, call a cab and remember their own school assignments, the better. In my...

  49. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... thereafter, using mouse instead of human AAG (remember, MPG and AAG are the same protein!) (Chen et al...A•T-to-C•G base editing in mammalian cells and embryos. Nature Biotechnology, 42(4), 638–650. https://...

  50. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...produce ultrasound contrast. That is, until earlier members of our lab - the Shapiro Lab - developed Acoustic...student studying bioengineering. They are both members of the Shapiro lab at Caltech.   References and...
Showing: 251 - 300 of 510 results