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Showing: 101 - 120 of 155 results
  1. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...mind that our customer support scientists wear many hats at this still relatively small nonprofit. If you...
  2. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ...expression systems including their advantages and caveats to keep in mind before choosing a system. Mammalian...
  3. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...that were previously difficult to engineer, like rats. If you have tools for creating knockout animals...
  4. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...then cleaving the ssDNA strands. In this way, Cas3 eats away at both the non-target and target strands by...
  5. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost entirely non-...
  6. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...targeting the viral genome or inverted terminal repeats (ITR). You should always have a high-quality plasmid...
  7. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...Hardly! 9E10’s accessibility and the variety of formats you can find it in likely mean it will remain an...
  8. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... such as viral plasmids with inverted terminal repeats. We use NEB Stable to transform and prep high quality...
  9. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...with 3DISCO to image non-transgenic animals such as rats (17).  In addition, the use of viruses allows much...
  10. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...flowers inside. 50 mL falcon tubes or plastic weigh boats are both great options for this, depending on the...
  11. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...adenine to inosine, which the translational machinery treats like guanine. This RNA editor would permit point...
Showing: 101 - 120 of 155 results