... by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high...high-throughput profiling New England Biolabs® (NEB) researchers recently published a method for the high-throughput...blogger, Greg Lohman! Greg Lohman is a biochemistry researcher at New England Biolabs, specializing in the enzymology...Thermus thermophilus DNA ligase." Nucleic acids research 24.15 (1996): 3079-3085. PubMed PMID: 8760897....in duplex DNA by T4 DNA ligase." Nucleic acids research 10.5 (1982): 1425-1437. PubMed PMID: 7041091. ...can be sealed by T4 DNA ligase." Nucleic acids research 15.21 (1987): 8755-8771. PubMed PMID: 3684572....mononucleotide repeat sequences." Nucleic acids research 27.24 (1999): e40-e47. PubMed PMID: 10572192. ...

...idea of the themes in the field, I searched for recent research papers on these particular themes, for... your ability to write, to read efficiently, to search the literature, and to synthesize a large volume...particular topic. Alternatively, maybe you’re researching a topic for which no relevant or recent review... the field you plan to review. If it’s a well-researched field, you may need to get specific to make sure... has the benefit of exposing you to relevant researchers in the field. Finally, you can sound credible...an expert in the field. To find reviews, I just searched online and found ones that I thought “looked good...cared about. I also used reviews to get a list of research papers that I needed to read. Once I had an idea...

...distribution of plasmids for COVID-19 research is accelerating essential research in laboratories all around the...sometimes wonder if we can take another blow. Back in March, we never expected to be in this here and now. Our...Here's the message I shared with the Addgenies on March 11, 2020 after rumors spread in our building that...scientists around the world mobilized on COVID-19 research, cures, and diagnostics. Almost immediately we...proud of the work we’ve done to support COVID-19 research. Our collection of available materials is growing...the crucial role Addgene plays in accelerating research. Yet this was still not enough to ensure we could... doing almost every type of biomedical science research. Our work accelerates and enhances productive ...

...individuals whose research ranged from wildlife conservation to abortion law to poetry. A research discipline...not ultimately translate to a desire to do the research itself. Several years of exclusively writing lab...depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps; and an essay on the life...versatility in the face of disparate spheres of research. In my case, an interest in neuroscience was carried...occupied my attention, until these coalesced into a research topic on concussion in sport and the public’s ...sport my entire life, I was curious as to what the research had to say. Moreover, this subject allowed me ...public utilizes insights gleaned from scientific research is, after all, one of the enduring goals of science...

...and an inability to improve upon these critical research tools. Addgene is excited to announce that we ...problem.The grant, which is part of the NIH’s Brain Research through Advancing Innovative Neurotechnologies...high-quality antibodies/nanobodies for neuroscience research and is an expert at validating these tools. While...offer numerous advantages, including more secure archiving, unambiguous molecular definition, and potential...the most commonly used reagents in neuroscience research today, but there is a critical unmet need for ...financially self-sustaining through distributions of research materials, such as plasmids and viral vectors....acquisition of affinity reagents and will enable researchers everywhere.” The timing is ideal as the NIH’...

... Overview of the CRISPR classification hierarchy. The hierarchy flows from Class to type to subtype to...a decade of frenzied interest in bacterial and archaeal immune systems. During this time, scientists discovered...perhaps provide insights and ideas for your own research! Classification Structure Principles of CRISPR...fast evolution of these adaptive bacterial and archaeal immune systems has led to extreme sequence divergence...classifications were placed into a fairly simple hierarchy: class to type to subtype to variant. Despite ...seemingly complicated steps to arrive at this hierarchy, the main aspects of each class and type are easy...identified CRISPR-Cas in bacteria and nearly 100% in archaea, though Class 1 remains largely underutilized compared...

...proteins have enabled scientists to pursue creative research avenues previously unavailable to them. With these...interactions. Beyond these common applications, researchers are finding new ways to apply fluorescent proteins...Roger Tsien played enormous roles in enabling researchers to utilize and develop these tools by making ...fluorescent protein technologies widely available to the research community. To honor their legacy, we recently ...help scientists share their fluorescent protein research with the academic community by funding their travel...demonstrated the utility of these FLiPs in a variety of research applications and particularly notes their ability...inducible enzyme technologies. She hopes that other researchers will be able to use similar designs to create...

...publication.   Do research! Nearly everything we write entails a primary literature search to collect up ...webinars, or use other research methods. In the case of the antibody guide, primary research and previously ...easily and quickly access tools to further their research. However, tools aren’t very useful if you don...goal is to provide informative content to help researchers in creating and sharing knowledge, related to... types of materials we help distribute and the research communities that we serve. How we curate (and...you didn’t know you wanted to know about, like research breakthroughs or cool new tools. And while this... Center, help.addgene.org, where you can easily search articles answering all of our frequently asked ...

... the progress of research, they can also present hurdles for the team of researchers involved. Communication... where researchers spent time in each other’s labs. This is an opportunity for the researchers involved...Communication is a major challenge when researchers are communicating across disciplines or doing different...or Google Hangouts is a great way to include researchers from different locations. Having virtual face-to-face...best for them. This also accelerates the pace of research by circumventing the slower, sequential communication...document. Set and manage expectations When the researchers involved in a project are scattered across the...future directions of the project. The state of research is constantly shifting, and long-distance collaborations...

...describe all cell lines used in research, using four methods to search out cell lines: Periodic surveying...approximately one-third of cell lines used in scientific research are misidentified! Rigor and reproducibility of...collections websites and catalogues Literature searches utilizing Google Scholar alerts and the NCBI LitSuggest..., such as their vaccine production, SARS-CoV-2 research, or adenovirus packaging cell lines dataset collections...was described. Using Cellosaurus Cellosaurus is searchable by cell line name (recommended names and synonyms...You can also use the Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your...adjusted based on query, reference, and shared allele search preferences to help you identify proximal lines...

...pAAV.hSyn-FLEX.iGABASnFR.F102G pAAV-Syn-Archon1-KGC-GFP-ER2 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] pAAV-FLEX-tdTomato...constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) ...iGABASnFR and its F102G variant in AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical ...brain was measured with implanted electrodes. The Archon1 voltage reporter allows a way to monitor neuronal...imaging. Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction with optogenetic...this membrane-embedding voltage detector. Find Archon1 and its cre-dependent version at Addgene! Fluorescent... viral protocols and videos Find AAVs for your research ...

...Having seen CRISPR’s success in basic research, researchers are eager to apply it in a clinical setting...Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your Research ...preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections Pages Duchenne muscular... around 30 years of age. Despite the amount of research conducted on DMD, there is still no good treatment...it difficult to deliver via gene therapy, so researchers have set their sights on another approach. Since...oligonucleotide treatment comes with many challenges, researchers have begun to explore genome editing approaches...CRISPR-mediated exon skipping approach in mice, DMD researchers are very excited. This approach may also be applicable...

...skills required Research skills The ability to think critically and carry out in-depth research is applicable...career, do your research Once you have identified possible careers, the next step is to research what each ...Epidemiologist Scientific Recruitment Meteorologist Research and Development Healthcare Consulting Lab Manager...Manager Patent Lawyer Science Market Research Statistician   Attention to detail Patience, accuracy...include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor Lab Manager...Before you begin to apply for a job, it is useful to search online job websites to see what requirements or...Once you’ve assessed your skill set and done your research, it’s time to start sending out applications. ...

... CRISPR technologies available to the academic research community. At Addgene, we’ve helped distribute...provide ready-to-use CRISPR lentiviral preps to researchers across the globe. Why provide CRISPR as virus...proceed to use it for experiments.” - Alex Chavez, researcher at the Wyss Institute for Biologically Inspired...can make lentivirus production frustrating - a researcher can complete an entire lentiviral production ...receiving a pooled gRNA library in plasmid format, a researcher must amplify the library, use next-generation...reagents from Addgene can greatly expedite a researcher's ability to make discoveries." If you ever run...plasmids to create your own lentivirus stocks. Our research scientists have provided their standard lentivirus...

...focused on translational research is encouraging. Patients are key to the research, but they are also the...observations regarding what makes this group of researchers special: 1. Stem cell scientists care deeply ...Listen to Our Podcast Interview with Stem Cell Researcher Darrell Kotton 2. Stem cell scientists care about... on spinal cord injuries; Jessica Young shared research on stem cells and Alzheimer's Disease; and Kevin...the reason this research is happening. Stem cells scientists have certainly not lost site of that. 3. Stem...acquisition is not a rate-limiting step in stem cell research. People told me they couldn't have done their ... to helping the stem cell community with their research, I think I can speak for Addgene when I say that...

...Information DatabaseResearcher: Amos BairochViral Vectors 101: Systemic CapsidsResearchers: Cynthia Arokiaraj...work with Addgenie writers to help share their research, tools, and knowledge. Guest authors The Codon... Benedict Diederich Course-Based Undergraduate Research Experiences (CUREs)Author: Jason PellettieriNew...NEBGCE4All: Making Genetic Code Expansion AccessibleResearcher: Ryan Mehl; Andy KarplusIBEX Knowledgebase...Knowledgebase: A Data Resource for Multiplex Tissue ImagingResearchers: Andrea Radtke, Ziv YanevCellosaurus: A Cell...ShayMultifunctional Neuronal Fibers: Modulation from Gut to BrainResearchers: Atharva Sahasrabudhe; Polina Aniveeka   A...blog this year! Your contributions have helped researchers around the globe find and share knowledge, resources...

...statement: Accelerate research and discovery by improving access to useful research materials and information...post was contributed by Kyle Hooper at Promega. Researchers have been sharing plasmids ever since there were...examples of technologies that can accelerate your research. The creation of NanoLuc® Luciferase from a rather... or above 600 nm wavelengths should be used. Researchers often use bioluminescence to overcome these issues...brightness of NLuc has caught the attention of researchers wanting to perform whole-animal imaging, however...leads to spurious activation of the FRET sensor. Researchers have sought to alleviate many of these challenges...also activate the optogenetic accuators. The researchers took a FRET reporter and adapted it into a BRET...

...Novartis researcher, Carrie Bhang. The ClonTracer Library, deposited by Carrie Bhang, a research investigator...was actually really research oriented [and] somewhat similar to an academic research environment” with ...Oncology, is an exciting new tool that allows researchers to individually label millions of mammalian cells...because she wanted first-hand experience in industry research. When she came to Novartis, she was happily surprised...easily be missed. The ClonTracer library allows a researcher to label individual members of a starting population...she wanted to make it as easy as possible for researchers to use this tool in their own work. She says,...resource to the Addgene community! Carrie Bhang is a research investigator in In Vivo Pharmacology at Novartis...

...various light-controlled CRISPR tools available to researchers - some readily found at Addgene. Shining light...position to activate gene transcription. The researchers tested a variety of combinations to optimize ...pattern during blue light exposure (470nm), the researchers showed that expression of the human ASCL1 gene...cells to induce expression of human IL1RN, the researchers saw an 11-fold increase in mRNA levels after ...cycled on-off-on. Using a slit photomask, the researchers also demonstrated the ability to spatially control...-mediated CRISPR labeling to study chromatin architecture (Hoffman et al., 2020). Fluorescent dCas9 proteins...can potentially alter the genome and chromatin architecture, it is useful to regulate the time in which ...

...Addgene to accelerate their research. Read on to learn how these researchers plan to use plasmids from ...Genomics Institute to provide plasmid grants to researchers working on rare diseases through the BeHEARD ...from Addgene to advance their research on diseases of glycosylation, arginine mutagenesis, and neural development...She leads the Glycoimmunology group based at the Research Unit on Applied Molecular Biosciences (UCIBIO)... (APCDG) and an international platform for CDG research, named the CDG&Allies – Professionals and Patient... Colorado, USA. The primary focus of his lab’s research is the mechanisms by which proteins assemble into... a professor at the University of Buffalo. His research focuses on understanding the structural determinants...