...also be designed with other DNA-binding domains, including the commonly used LexA and rTetR systems such ...could be delivered throughout the nervous system, including the motor cortex, cerebellum, and spinal cord....easily adaptable to neuroscience applications, including optogenetics, and they should make it much easier...Other proteins may also be utliized as scaffolds, including the set of red fluorescent proteins. The T-DOGGs...

...fat-soluble hormones, vitamins, and dietary lipids. Included in this family are receptors for endocrine steroids... to treat a multitude of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular...these key regulatory molecules, and has provided clues to their previously uncharacterized cellular roles...interface, expanded numbers of curated molecules, including 400 or so coregulators, and a greatly expanded...

... delivery technologies have been investigated including lentiviruses, adenovirus, and inorganic delivery...additional safety benefits: they lack all viral genes, including those that are responsible for integration into...capable of infecting a wide range of cell types including but not limited to muscle, liver, and brain cells...clinical trials have been directed at diseases including but not limited to hemophilia B, LPL deficiency...

...contains both fragments of split mVenus (aa 155), includes I152L in N-terminal fragment An enhanced recombinant... Dan Mulvihill mVenus Split mVenus (aa 155), includes I152L in N-terminal fragment Constant rate of ...  When designing experiments, it’s important to include proper controls to assess the behavior of the split...Biosensors Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein tools...

.... This tool-kit includes an array of FPs as well as additional useful tags, including 3xFLAG epitopes,... chromatin. A fluorescent protein can still be included for visualization purposes, but the key component...green. This system can be used in many cell types including endothelial cell-pericyte, neuron-astrocyte, and...

...for affecting change. On Friday, the symposium includes a panel discussion and keynote by former congressman...implementation and compliance for the FLSA changes, including finding money in already tight lab budgets and...their advocacy skills. Friday’s symposium will include a discussion on effective advocacy strategies on... on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor MIT Washington ...

...unstained flow controls). Spectral flow cytometers include a tool called “autofluorescence extraction” which...various tissue types varies, it is critical to include an unstained sample for each tissue type you are... the expression levels of the markers you are including in your panel. Highly expressed markers should...during unmixing. Additionally, it is critical to include unstained beads or cells within your reference ...

...Over the years, Addgene’s repository has grown to include many CRISPR tools.  Distributing and depositing...this tell us? Well, it’s hard to draw any exact conclusions, but it certainly suggests that Addgene depositors...approximately 104 plasmids shipped per working day, including appropriate MTAs and customs forms. Of course,...repository have been cited a total of 23,006 times, including 14,677 unique citations.  CRISPR is a story that...

...year’s Nobel Prize in Chemistry, he joined an elite club, becoming just the fifth repeat Nobel Prize winner...proposed as a mechanism for increasing diversity and inclusion in STEM disciplines (Bangera & Brownell, 2014;...promote professional success (many of my students include their in-class research experience on their resumes...experiences can make scientific research more inclusive. CBE Life Sciences Education. 13(4):602-606. Brian...

...proteins have replicated many of the properties once exclusive to small-molecules: red-shifted spectra, ion sensitivity... have been developed based off of this concept include: Self-labeling tags (e.g., SNAP-tag, HaloTag, ...innovative labeling strategies, several groups—including mine—have been revisiting the old chemistry of...and contain polar groups (Figure 2b), which can preclude their use inside living cells. We recently discovered...

...beer. Ha! We had over 60 people join the fun - including a lot of future Addgenies as shown in the photo...employees. We now have a team of three (myself included) that plan big company events and fun things to...people you spend 40+ hours with each week. In conclusion - get out there any have some fun together! Laura...at all really! We've had plenty of events that included a simple corn hole set and a cooler. Chances are...

... flexibility is their broad range of targets, including proteins, peptides, amino acids, drugs, metal ...biosensors are used in a variety of applications, including detection of environmental pollutants, drug discovery...aptamers for use in many entirely new applications including aptamer based fluorescent biosensors. Aptabeacons...-based instead of protein-based fluorophores, including: Environments with higher temperatures, since...

...you start reading, there will be a temptation to include every piece of information that was ever published...figures/schematics or tables. And don’t forget to include citations so that people can go back and read the...studies that reported mutations in that protein (including how frequently a mutation was found and the size...doesn’t have to be revolutionary, but you could include a few comments on where you think the field is ...

...the CRISPR workflow. For a multiplex sample, ICE includes visual representations of all detected edit types...indel.   For a complete guide to ICE analysis, including detailed explanation and examples, read the paper...of the DNA-targeting region of the guide RNA, excluding the PAM sequence. PAM Sequence - The Protospacer...stack Genome Engineering Solutions, which also includes our CRISPR knockout guide design tool, guide validation...

...an interview next week and it includes a chalk talk. What should I include in my chalk talk?" So what exactly...proposed approach to resolve the knowledge gap? This includes the overview and the individual aims. Describe...Howard Hughes Medical Institute. His interests include the quality control of secretory proteins in the...

...made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~3.3 kb) from Streptococcus... approximately 1350 amino acids in size, which includes SpCas9, and shortern orthologs approximately 1000...in mouse liver.  Two other smaller Cas proteins include Cas12b and CasX. While there are no papers yet ...capabilities of full-length SpCas9. This set of plasmids includes AAV-Cas9C-VPR for targeted gene activation. Split...

... hardest part of any revolutionary discovery, including CRISPR, is turning potential into impact. In molecular..., PhD, and Jonathan Gootenberg, PhD, focuses exclusively on this space — developing tools for cellular...measurable cell toxicity – using Cas7-11. This included tests done in cell lines known to be more sensitive... — the ability to now make any small changes, including base edits, insertions, and deletions. But CRISPR...

...an X,Y axis to generate a dot plot (Figure 1). Clustered events are called a population.                           ..., like a box, ellipse, or circle, drawn around clusters to define populations. This shape is called a ...diameter and volume of the cell, thus allowing the exclusion of doublets and debris. The side scatter roughly...show frequency of events, instead of relying on clustered dots. In a contour plot, each line contains the...

...inside the body. So we screened gas vesicles gene clusters from a diverse set of gas vesicle-expressing species...right: colony whiteness on inducer plates, white inclusions in cells with GVs via phase contrast microscopy...this was the Anabaena flos-aquae gas vesicle gene cluster because of its small size and its ability to produce...a time-intensive endeavor. That is why we also included an analogous set of transient constitutively-expressed...

...domain - VP64.  Later generations of activators include the SunTag system which facilitates recruitment...Addgene’s website. Fig. 3: Cas9 repressors include dCas9 alone and a dCas9-KRAB fusion, both targeted...targeted to the promoter region. Cas9 activators include Cas9 fused to VP64, VP64 along with other SAM factors...expression in different species and cell types including (but not limited to) bacteria, yeast, plants, ...