We narrowed to 600 results for: ELP

...and viruses, from crossing into the brain. While helpful from a biological standpoint, it makes delivering...information about commonly used and emerging capsids to help you decide which ones may be suitable for your experiment...area of interest, and health. While it’s always helpful to start with a literature search to see what others... promoters and enhancers (Mich et al., 2021) can help restrict expression to your cell type of interest...just reduces, empty viral capsids in your prep can help reduce the risk of an immune response. Strain-specific...table.     Figure 2: This decision tree can help guide your choice on which systemic capsid to use...options for systemic capsids, but we hope this post helps you find the right one to test in your application...

...there's a chance it'll never work. If you help others out, they'll help you too; both with your research and... not a fluke.” 3. Engage in various projects and help colleagues with their work. “Your one project may...with this passion. “Love what you are doing!” It helps when the time comes to work with full force. George...

...genome engineering, and studying its structure can help us understand the parameters of CRISPR experimental...arginine-rich bridge helix connects the two lobes and helps recognize the guide RNA. Unlike the cleavage domains...the CTD, ready to check for PAM sequencines. This helps explain why Cas9 can’t locate PAM sites until after...correctly before HNH or RuvC can cleave DNA, which helps limit off-target activity. What next? It’s not actually...different uses. In all cases, structural biology can help you visualize the system you’re working with and...

...techniques and tools that helped make my experience run smoothly and can help other teams successfully ...results or ask questions to your collaborators. This helps everyone feel included in the project and accelerates...members of your lab that you see in person, and helps improve your interpersonal relationships with people...

...for Publication policy with two changes that will help us ensure that plasmids don’t get left behind in...plasmids available as soon as your paper is published helps maximize the potential impact of your work. Plus...and waiting for replies. This updated policy will help our teams have clear workflows and keep things moving...questions about depositing, check out our website, Help Center, or email us at [email protected]. ...

...like to help share useful new resources with the scientific community – particularly ones that help address...lines you are currently or planning to use for helpful information on the known features and relevant ...reference, and shared allele search preferences to help you identify proximal lines you care about. If you...

...now has a helpful graphic to introduce you to the plasmid class covered on that page. To help you find ...also give you high-quality educational resources to help you do better research. CRISPR is an incredibly ...research toolbox, as well as additional resources to help you Plan Your Experiment. #12DaysofCRISPR and blog...

...this in the future. Here’s a list of blog posts to help you tackle the challenges of DNA repair (with bonus...how a better understanding of Cas9 biophysics can help enhance HDR. CRISPR 101: Ribonucleoprotein (RNP)...genome and just stick to RNA? Cas13 may be able to help you do that! How to Design your gRNA for CRISPR ...had great things to say about CRISPOR, a tool to help you design, pick, and close guides for Cas9 and ...

...closely with TTOs to help protect our depositors’ intellectual property, and to help requesting institutions...you requested from Addgene...the plasmid that will help you run that last additional experiment requested...commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology Transfer Information...

... While we love academic research, we hope we can help all researchers achieve happiness in their careers...Scientists: Why and Where Do I Find One? If you need help making a transition into a new job or maybe to an...new career path, a career coach might be able to help you. Head Addgenie Joanne Kamens explains what career...engineering. 10. Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need...

...has certainly noticed: This year, the BeHEARD (Helping Empower and Accelerate Research Discoveries) Award...and his colleagues at the Wistar Institute in Philadelphia, PA will put their award to work on a condition...different variants and strategies out there. This will help us acquire a lot of those plasmids so that we can...after periods of high activity. The new work will help them to understand how different mutations lead ...

...CRISPR, there are two major areas where software can help. The foremost handles the design of sgRNA and represents... Benchling. Utility functions: These functions help speed up the sgRNA design process by removing repetitive...repetitive tasks and/or by providing features to help with the post-design process such as primer and ...applying CRISPR technologies to new organisms and will help you whether you have a full assembly or not. It ...to re-evaluate all tools. Report your findings to help everyone else. CRISPR technology has reached into...

...).  Evaluating a drug’s immunogenicity in-vitro helps researchers understand a drug’s safety and efficacy...silico and in vitro assessment of immunogenicity can help evaluate risk of an antibody.  In silico prediction...Treating these cells with the therapeutic antibody can help us assess the effects of the drug on the actual ...in silico method of immunogenicity assessment can help in determining the T-cell epitopes that will likely...

...bench mentor or anyone else available to directly help you during your visit. Reach out to your bench mentor...comfortable initiating this conversation, your mentor can help get the ball rolling! If you are applying to a structured...Remember that your host is going out of their way to help you with your research and teach you new techniques...questions if you don’t understand something. Offer to help out wherever you can and don’t forget to express...

...atlas, including over 3,500 human TF isoforms, to help researchers understand this regulatory network. ...mechanisms, although the unique barcodes for each TF help streamline identification. While the Zhang lab controlled...overexpression programs on cellular processes. For help with library transformation and amplification, refer... yet available, just ask an Addgene scientist at help@addgene.org! References and Resources  References...

...these tips and resources collated by Addgenies to help you keep calm and science on. Practice self-care...mental break, while meditating right before bed will help you prepare for sleep. At Addgene, our mindfulness..., you can contribute data to the AAV Data Hub to help the research community. Set expectations for your...overwhelmed right now, but hopefully these tips are helpful to you no matter what you’re trying to accomplish...

...catalog number for identifying a reagent can be helpful, but it lacks context unless it links to a verified...sequence information, and more. The plasmid page also helps researchers find other publications that have used...Having IDs linked to these metadata and publications help to enable reproducibility of experiments. Additionally...services such as Identifiers.org and N2T.net. To help authors comply with journal policies, Addgene now...

...a selection strategy is not enough on its own to help you find your desired plasmid. In many cases, you...a screen to your cloning strategy? A screen will help you more easily identify successful clones so you...restriction digests to analyze plasmids, which can help you visualize this process, or visit our restriction...just 1 kb of DNA, custom primers are especially helpful in verifying mutations that wouldn't be reached...

...finding ways to intentionally set up your bench to help manage details. For example, setting up your pipette...pipette tips to match your plate layout can help you track samples as you add them. Moving your reagents...your left side to your right as you use them can help you remember what's been added and what hasn't. ... as you work, and the possibilities for small, helpful changes are endless.    Fig. 2: Blugene...

...cells and molecules are recruited by the antibody to help destroy and remove a pathogen. Different isotypes...system proteins, which can initiate a cascade that helps attack extracellular pathogens.  Transportation ...: IgG1, IgG2, IgG3, and IgG4. IgE IgE antibodies help protect against parasites but also play a role in...isotype heavy chain constant domains. This switching helps B cells adapt during the course of an immune response...