...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...double-stranded break. The Xiao Wang Lab has recently deposited a set of plasmids that helps identify base edited...plasmids use “Cas9-mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify...CRISPR plasmids are always being added to the repository. To find all of the CRISPR plasmids available...

... plasmids in the repository, each verified by sequencing, which makes the repository a convenient source...genes deposited at Addgene were synthetic. But nine years later, over 20% of the genes deposited that ...most common expression system within the Addgene repository is mammalian, but the largest source of unique...

...Page Thank you to all of our generous, sharing depositors. Over 3,000 laboratories are sharing plasmids...currently sharing viral preps) through the Addgene repository. Without these scientists and the support of ...had to pave the way for this project with many depositors and partners. The Addgene Scientist Team has ...Team acquired permission from relevant plasmid depositors and their institutions to distribute viral preps...preps derived from plasmids in the repository. The Outreach Team has been making sure scientists know ...

...have perfected processes to curate the data our depositors share and enrich it with sequencing data; as ...categories — catalog, educational resources, and depositors — each with subcategories such as plasmids and...educational resources, and labs and institutions for depositors (see below). Even if the user experience is similar... match. For example, matches of a name in the depositor field will rank higher than matches of the same...that contains a plasmid with the catalog ID). Depositor suggestions provide shortcuts to matching PI labs...

...If you’re depositing a pooled library with Addgene, you may be surprised to learn that we ask for an ...an amplification protocol with your deposit. This is because repeated amplifications of pooled libraries...amplification protocol for your pooled library deposit. When a requesting scientist receives a pooled... ensure that it works for your library before depositing it with Addgene. If you have any specific questions...amplification protocol, feel free to contact us at deposit@addgene.org. Happy amplifying! More resources ...

...step. Scorpion chemistry in particular has been reported to work well in the Lightcycler while being relatively...Yes Agarose gel Sunrise primers   Low Reporter and quencher on hairpin structure included in ...much as sixfold variation in expression levels reported (Biassoni, 2014), so you’ll want to ensure that...conceptualize. Just remember to switch them back before reporting the data!   Statistical analysis  Getting...frown upon statistical laxness in all its forms. Reporting your data  Once you’ve done the work to produce...(Bustin, 2009) to ensure you’re recording and reporting the right information so others can reproduce ...

... the names. We also have many plasmids in our repository that you can use to study these genes! Groucho... contain the groucho gene? Check out plasmids deposited by the Shivdasani Lab from the Dana Farber Cancer...here! The most popular SHH plasmid however was deposited by none other than Cliff Tabin himself.   Sleeping... see for yourself. We have one plasmid in our repository, plasmid 39624, that contains cmr.   tinman and...on Addgene.org Find plasmids for your research Deposit plasmids at Addgene  ...

...readers may locate data supporting the research reported in PLOS publications. The policy was not an overnight...is calculated and placed in the legend. Widely reported difficulties in reproducing experimental data ...datasets are too large to transfer to a journal repository, they are likely too large to be retrieved by...Scientific Data, which aims to act as a peer-reviewed repository for “scientifically valuable” datasets. It likely...likely won’t be long before other data repositories emerge that allow efficient searching and extraction...

...only 46% could be reproduced. In a recent Nature report from researchers at Amgen, it was stated that only...through all saved data and automated writing of reports. To be able to understand the situation in depth...keep paper records as well as digital, or for reporting on a project – printing your data out can be required... you might need share data with them including reports and protocols. To facilitate this, some ELNs allow...Software for Biology Resources on the Addgene Blog Deposit your plasmids with Addgene and make your work more...

...Russell and Hirata from the University of Washington reported a surprising finding - when delivered via AAV ...take up ~6.5 kB if delivered in a single AAV. They report roughly 40% repair efficiency in the K562 leukemia...develop AAVs for your own research and encourage to deposit any new AAV tools you create. Many thanks to our...AAV vector cures haemophilia B mice." Scientific Reports 7.1 (2017): 4159. PubMed PMID: 28646206. PubMed... gene cassettes using AAV donor vectors." Cell reports 20.3 (2017): 750-756. PubMed PMID: 28723575. PubMed...

...Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope ...pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for more information Miguel Sena-Esteves...pcDNA-SADB19L Contains rabies viral RNA polymerase; see depositing paper for more information Edward Callaway ...tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab....

... When we started in 2004, we were the plasmid repository. In 2016, we added viral vectors, and in 2022...ready-to-use antibodies. Now, we think of ourselves as a repository for DNA-based materials, including our popular... kits and libraries. This DNA-based materials repository contains 139,868 reagents from 5,821 labs in ... system for scientists using materials in our repository, from our customer and science support to our...

...at specified loci. Charles Gersbach’s lab has deposited mammalian expression constructs including pcDNA-dCas9...Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9-DNMT3A-EGFP and pdCas9-DNMT3A-PuroR for...Methyltransferase MQ1 Margaret Goodell's lab has deposited pcDNA3.1-dCas9-MQ1(Q147L)-EGFP, a fusion of dCas9...Demethylase 1 (LSD1) Tatjana Sauka-Spengler's lab has deposited pX330a dCas9-LSD1 for targeted removal of H3K4me1...-dead Staphylococcus aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. Here's what... intensity-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren ... license. While the GENIE team tested and deposited versions with both PDGFR and NGR fusions for membrane...optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of one of seven enhancers...interneurons in mice. A) AAV expression of dTomato reporter (magenta) under the control of the BiPVe3 enhancer...

... fans of all our depositors here at Addgene — your contributions make the repository what it is. So we...we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry...pressures during protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along with ...

... SHERLOCK (specific high-sensitivity enzymatic reporter unlocking) to detect single molecules of RNA or...transcription factors with GFP Kevin White’s lab recently deposited a set of CRISPR plasmids to tag human transcription...If you have a new CRISPR tool you’ve recently deposited to Addgene and you’d like it to be included in...Find CRISPR plasmids by function Find recently deposited plasmids ...

...Our plasmid repository succeeds because of our amazing community. The trust of our depositors to allow us...more scientists outside of the US now use the repository – about 50% of plasmids are shipped to labs outside...manage our website, and continue to improve the repository. We love being able to provide a useful (and ...

... to all articles, Anurag Acharya and colleagues report, the percentage of citations to articles in non-elite...publications. (As an interesting aside, Nature recently reported that the most cited work in history is a 1951 ... the best way to measure impact? As that Nature report also noted, papers detailing Nobel Prize-winning... for the impact of research" (2013) Scientific Reports 3, Article number: 1649 doi:10.1038/srep01649 ...

...We’ve dug into the data from our repository to find our most requested plasmid in 2015. In line with ...that the plasmid most requested from the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR...fantastic research tool has been requested from the repository nearly 1,000 times in 2015! If you haven’t already... labs all around the world. We thank our many depositors for making these reagents available to the research...

...visualize them. You can also “hear” them. Addgene depositor Dr. Mikhail Shapiro from Caltech presented an ...ultrasound to image bacteria in vivo: acoustic reporter genes’ on our blog. Creative approaches to presenting...References 1. Bourdeau, Raymond W., et al. "Acoustic reporter genes for noninvasive imaging of microorganisms...Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology Plasmids with Addgene ...