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We narrowed to 367 results for: ERG

Showing: 101 - 120 of 367 results

  2. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... luciferase substrate, the luciferase transfers energy to the FP, resulting in a fluorescent signal. Since...based on the principle of bioluminescence resonance energy transfer (BRET). Photons emitted by a Renilla luciferase...

  3. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ... products are then transformed into E. coli to undergo nick repair. This method can work well, but poor... starts out in familiar territory, but quickly diverges from previously discussed methods. As described...

  4. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    Published
    April 16, 2015, 3:45 p.m.
    ... Dept. of Biochemistry and Biophysics and the Lineberger Comprehensive Cancer Center at UNC Chapel Hill...j.molcel.2014.08.002 Ran FA, Hsu PD, Lin C-Y, Gootenberg JS, Konermann S, Trevino AE, Scott DA, Inoue ...

  7. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...batch to batch variability. Once an animal has undergone a terminal bleed to acquire all of its serum, ...https://doi.org/10.2144/btn-2018-0065 Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold...

  8. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ... by ion channels and pumps in the membrane. An emerging interdisciplinary field of biology known as bioelectricity...Developmental bioelectricity is an extremely exciting emerging field, with many implications for regenerative...

  9. Transferable Skills Guide: Public Speaking

    Type
    Blog Post
    Published
    April 20, 2023, 4 p.m.
    ...learned along the way. Own your time When I was an undergrad, I was assigned a paper to give as a journal club... of a very confused group of visiting Chinese undergrads. You get nervous and freeze, or forget a point...

  10. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a method for the visualization...14166766. PubMed Central PMCID: PMC300121. 3. Söderberg, Ola, et al. "Direct observation of individual...

  11. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites of the same type...recombination. I. Recombination between loxP sites. Sternberg, N. and Hamilton, D. 1981. PubMed PMID: 6276558...

  13. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ...the Auxin Inducible Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered ...  Plasmids for cGAL and split cGAL systems The Sternberg lab in 2016 created cGAL, a GAL-4 based bipartite...

  14. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...The stringent stripping solution uses heat and detergent to denature and remove antibodies but is also ...Tris HCl 62.5 mM Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%...

  15. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ... only hypothetical at this point. More time and energy must be devoted to studying Cas3 and its Cascade...S., Lin, C., Owen, A. L. G., Künne, T., . . . Sternberg, S. H. (2019). Harnessing type I CRISPR–Cas systems...

  17. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...tags into the genome of the pathogenic fungus Aspergillus fumigatus, which has been difficult to modify...mutagenesis by microhomology-mediated end joining in Aspergillus fumigatus.” Fungal Genet Biol. 86: 47-57. PMID...

  18. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer from one FP (e.g. CFP...

  20. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    .... Find plasmids from this paper at Addgene. Gootenberg, Jonathan S., et al. "Nucleic acid detection ...plasmids from this paper at Addgene. Abudayyeh, Gootenberg, et al. “A cytosine deaminase for programmable...
Showing: 101 - 120 of 367 results