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Showing: 101 - 120 of 246 results

  3. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...not need to be as high to achieve an observable signal in your experiment, therefore titer may not need...fluorescent proteins required to get a good fluorescent signal. It may not be necessary to concentrate this type...

  4. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ... Monoclonal antibodies can yield relatively low signal in immunoassays due to only having one binding ...you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal, with ...

  7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... set, we would expect TagRFP to give a brighter signal than mCherry. In general, filter sets designed ...all organisms and that if you want the brightest signal, you may need to try several proteins in your system...

  8. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    Published
    Dec. 15, 2017, 2:07 p.m.
    ...technique can be applied to an important neuronal signalling pathway - if you’ve got a useful tool, there’...neuronal networks, and selectively activating signaling pathways. Many scientists came to the Addgene ...

  9. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...now-common business practices, like accepting e-signatures and designing a website users could easily navigate...institutions with standardized agreements and electronic signatures to dramatically speed up MTA approvals. In 2013...

  12. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...antibody is ideal as it will help to amplify the signal. Some applications use either direct (primary-conjugated..., remember that a secondary should be used when signal amplification is needed.  Species  Next, check ...

  14. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator. Because of its...pdb-top069609. Palmer AE, Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators...

  15. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...Gutless Ad maintain only the ITRs and the packaging signal (Ψ), both of which are essential for assembly of...sequences and retain only the ITRs and packaging signal in cis. Therefore, co-infection with a helper adenovirus...

  18. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...  Figure 2: Specific binding of scFVs to cognate aaRS antigens as measured by suspension bead assay...enriched in the pre-synaptic terminal providing better signal to noise for imaging calcium dynamics in fine structures...

  19. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ...light to detect, measure, and control molecular signals, cells, and groups of cells in order to understand... into distinct populations based on fluorescent signal. Thus, FACS can be used to separate cells expressing...

  20. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

    Type
    Blog Post
    Published
    Jan. 24, 2023, 2:15 p.m.
    ...-expressing E. coli. The GV-specific ultrasound signal inside the tumors was overlaid with the anatomical... in situ expression. The GV-specific ultrasound signal is in hot and the anatomical B-mode images are ...
Showing: 101 - 120 of 246 results