We narrowed to 253 results for: IRES

1. How Do I Place an Addgene Order Using a PO Number?

   Type

   Blog Post

   Published

   Feb. 27, 2020, 2:15 p.m.

   ...copy of your PO. If your purchasing department requires a quote in order to generate a PO, learn more ...

2. 6 Tips for Analyzing and Troubleshooting Sanger Sequencing Results

   Type

   Blog Post

   Published

   June 26, 2014, 2:23 p.m.

   ...for DNA sequencing If your sequencing facility requires you to perform your own Big Dye PCR amplification...

3. Designer PUF Proteins for Any RNA Target

   Type

   Blog Post

   Published

   June 17, 2014, 7:57 p.m.

   ...RNA. As a result, targeting the RNA of interest requires that the RNA be tagged. “This tool makes it possible...

4. Let There Be LITE Plasmids

   Type

   Blog Post

   Published

   Nov. 21, 2013, 3:56 p.m.

   ...environments. We are able to react and learn. All of this requires genes to be regulated dynamically.” To understand...

5. Phage Directory: From Phage Therapy to a Repository of Phage Information

   Type

   Blog Post

   Published

   Jan. 10, 2019, 2:38 p.m.

   ... archive phage reagents. If a phage researcher retires, it's likely that the phages are lost if they are...

6. Easing the Protein Purification Process with pCri

   Type

   Blog Post

   Published

   June 19, 2015, 3:08 p.m.

   ...stressful lab activities. Working with proteins requires a substantial amount of properly folded, relatively...

7. Science as Art

   Type

   Blog Post

   Published

   March 2, 2015, 3:13 p.m.

   ...The other thing photography has shown to me - it requires an awful amount of skill,  time for which most...

8. New Norepinephrine Indicators: nLightG and nLightR

   Type

   Blog Post

   Published

   Dec. 12, 2023, 2:15 p.m.

   ..., Wu, Z., Wu, H., Chen, W., Zhang, P., Zou, J., Hires, S. A., Zhu, J. J., Cui, G., Lin, D., Du, J., & ...

9. What's New in CRISPR - September 2019

   Type

   Blog Post

   Published

   Sept. 10, 2019, 12:56 p.m.

   ...gene) on the donor vector. Therefore, targeting requires two sgRNAS: one that targets the knock-in site...

10. Build Your CRISPR Vocabulary

    Type

    Blog Post

    Published

    July 2, 2024, 1:15 p.m.

    ... of an RNA guide (either crRNA or sgRNA). Cas9 requires perfect complementarity in this region between...

11. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type

    Blog Post

    Published

    June 11, 2020, 1:34 p.m.

    ...chemogenetics on the Addgene website. Optogenetics also requires that light of the correct wavelength reach receptors...

12. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type

    Blog Post

    Published

    May 21, 2020, 1:15 p.m.

    ...transgene (Lin et al., 2018). However, this system requires precise titration of the AAV. So far, tools to...

13. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type

    Blog Post

    Published

    June 11, 2019, 1:16 p.m.

    ...are under a Creative Commons 3.0 license, which requires users to give appropriate credit, provide a link...

14. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type

    Blog Post

    Published

    Nov. 1, 2017, 2:04 p.m.

    ...about kit cost and safety.  Being a manager also requires Caitlin to look at the big picture: “It’s been...

15. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

    Type

    Blog Post

    Published

    July 18, 2017, 12:41 p.m.

    ...acids are essential, which means that the body requires them but cannot produce them. By using yeast integration...

16. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type

    Blog Post

    Published

    Dec. 3, 2020, 2:15 p.m.

    ...Your next cool experiment requires some AAV.  Where do you start? Plasmids of course! You just need three...

17. Management for Scientists: What Makes a Good Manager Anyway?

    Type

    Blog Post

    Published

    Dec. 9, 2014, 2:22 p.m.

    ...time spent at the desk, but it is worth it. This requires a focus on deliverables. It does not mean a manager...

18. Advice for Choosing a Research Project

    Type

    Blog Post

    Published

    April 17, 2014, 2:45 p.m.

    ...approach. At Addgene, we've heard of labs where new hires spend the first 3 months reading and writing a proposal...

19. Summer SciComm: Science Storytelling

    Type

    Blog Post

    Published

    Sept. 16, 2022, 1:15 p.m.

    ...opposing forces or (2) the result of competing desires or (3) the presence of obstacles that need to be...

20. RUBY-Red Siliques

    Type

    Blog Post

    Published

    March 1, 2022, 2:15 p.m.

    ...plants, but screening these can be challenging and requires specialized equipment. Reporters for studying ...