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We narrowed to 461 results for: MEA;

Showing: 101 - 120 of 461 results

  1. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ... as real time PCR is a PCR technique used for measuring a starting DNA concentration using PCR. qPCR requires...fluorometer feature built into the thermocycler to measure that fluorescent output. With this fluorescent ...droplet is analyzed by a droplet reader, which measures the fluorescence amplitude of each droplet. The...

  2. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...specific antibody to the C-terminal five amino acids, meaning the tag could be quite small. However, substance...sequence context.    So, do Schüchner, et al.’s results mean the end of 9E10’s time in the saga of the Myc tag...the variety of formats you can find it in likely mean it will remain an important tool for the foreseeable...

  3. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...initiation site and open circles mark pixels used to measure signal along the dendrite. D) Fluorescence signal...signal as a function of the distance between measured pixel and the soma. The initiation site identified...Figure 4). Figure 4: ONE-GO biosensors measure the formation of Gα-GTP by BRET. The biosensor ...

  4. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...the expression level in a specific system (by measuring the fluorescence intensity); and 2) to visualize...activated by a specific excitation wavelength. This means that the emission wavelength can change. In a few...studies (24-30°C). Brightness: Brightness is a measure of how bright is the emission. Brightness is calculated...

  5. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    Published
    Sept. 28, 2017, 2:01 p.m.
    ...intensity and transduction efficiency of neurons were measured. On average, AAV.PHP.eB and AAV.PHP.S had 1.5-...1x1011 vg per mouse and the level of transduction, measured via GFP expression, was determined 3 weeks later...1x1012 vg per mouse and the level of transduction, measured via GFP expression, was determined 3 weeks later...

  6. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...as "the best way" to communicate their science, meaning that they will heavily evangelize for the mode ...and contextualizing data. Often overlooked as a means of scicomm. Digital or print distribution.  Typically...for conceptualization not possible through other means. Easy to combine multiple types of media. High barrier...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...level of resolution that they can achieve. This means that regardless of how powerful of an objective ...you image samples before and after expansion and measure the degree of agreement between them to ensure ...fragmented appearance post-expansion, but that doesn’t mean we should start re-conceptualizing actin filaments...

  8. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...Healthy plants produce lots of seeds. More seeds mean more chances to recover a transformant. Use young... expose the flowers to Agrobacterium. More seeds mean more chances to recover a transformant. Use lots...-10 plants per construct I transform. More seeds mean more chances to recover a transformant. Remove mature...

  9. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...viral constructs is a useful tool as it provides a means to quickly check that a construct is functional ... 2nd- and 3rd-generation vectors? What does this mean for transfer vectors? A4: The lentiviral packaging...Addgene transfer plasmids are replication-deficient meaning they can be used to create virus that is capable...

  10. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...eventually called aptamers, from the Latin aptus, meaning “to fit”. SELEX could be used to classify DNA aptamers...Making aptamers glow The flexibility of aptamers means they can be used with or as fluorophores in several...Fig. 3), such as Radish and Carrot, and further means to increase aptamer stability and decrease background...

  11. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    Published
    Sept. 17, 2019, 12:40 p.m.
    ...behavioral tasks was an elevated plus maze that measures risk aversion/anxiety. The mouse can move freely...lit (risky) and a dark (protected) area. When measuring the time spent in a particular part of the apparatus...46 (2014): 78-87. PubMed PMID: 24882160. Creed, Meaghan, Vincent Jean Pascoli, and Christian Lüscher. "...

  12. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    Published
    July 25, 2024, 1:15 p.m.
    ...et al. originally developed the ELISA assay to measure antibody levels, scientists have since adapted ...typically use a competitive or inhibition ELISA when measuring small molecules that cannot bind efficiently to...the amount of antigen in the sample: more antigen means less antibody available to bind to the reference...

  13. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression... revolutionized Neuroscience. They are the only means to target and manipulate specific cell types (populations... FLPe recombinase have been developed. A common means to achieve ligand control is to fuse a mutant estrogen...

  14. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...vector and internal control A transfection control measures transfection efficiency and enables observation...transfection control is an internal control vector, which measures transfection efficiency. An internal control may...aliquot/preparation. The replicate control isn’t measuring or assessing reproducibility of the effect of ...

  15. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...High-throughput DNA sequencing is then used to measure the frequency of each variant during the selection...contains their respective recognition site. This means that if the BbvCI enzyme used for ssDNA preparation...Douglas M., Jason J. Stephany, and Stanley Fields. “Measuring the Activity of Protein Variants on a Large Scale...

  16. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ... needs to be asked going forward is: How do we measure inclusion and equity? This is a difficult question...discluded/not treated equitably even when hired; well-meaning mentors often fail to judge the work of underrepresented...and participants of the symposium began providing means to answer some of these questions and provided ways...

  17. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...that you’re looking at institutes that have the means to support your work. For example, if your work ...Sometimes, “no we don’t have the funds,” really means they don’t have the money. If the department has...help you achieve your research goals, even if that means saying no to a negotiation or, in this case, to ...

  18. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...consequences in an experiment. For CRISPR work, this often means DNA cleavage at unanticipated sites. While off-target...experimental results and 2) perform targeted off-target measurements at those sites to get an idea of how high the...factor, etc.) this control is usually not very meaningful. Unfortunately, there really aren’t any effective...

  19. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...abilities, or other phenotypes that can be efficiently measured at the single cell level), are separated by, for...mouse libraries available through Addgene. This means you could perform a screen with one library from...can be used: Design a screen with an easily measureable cellular phenotype (e.g. survival of a cancer...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...GPCRs in the human genome are orphan receptors meaning their ligands are unknown. Identifying new ligands...Protein A tag. The luciferase activities can then be measured and normalized to assess interactions. Higher ...of CRISPR and AID technologies provides a clever means of investigating hard-to-study genes in hard-to-... work, they used the CRISPR/Cas9 technology to measure the contribution of each gene from the model apicomplexan...
Showing: 101 - 120 of 461 results