Chromoproteins: Colorful Proteins For Molecular Biology Experiments
Type
Blog Post
Published
Feb. 4, 2020, 2:15 p.m.
...high-copy plasmid-borne chromoproteins lost color overnight in liquid culture, though expressing the chromoproteins...for allelic exchange
Figure 3: pTOX plasmids expressing amilCP and tsPurple genes. Image: Lazarus...donor in HeLa cells.
Find ShadowR for mammalian cell expression here
Now that you’ve learned what chromoproteins...at the cells on the plate. For example, if your plasmid contains a gene encoding a blue chromoprotein, ...colonies to determine which cells have taken up the plasmid. Of course, other follow up methods like sequencing... chromoprotein sequences into E. coli BioBrick plasmids (meffRed, eforRed, asPink, spisPink, scOrange,...gfasPurple) (Figure 2).
Figure 2: Chromoprotein expression from bacteria on agar plate. Image: Liljeruhm...