We narrowed to 115 results for: N-pac

...2017).Walton, R. T., Christie, K. A., Whittaker, M. N. & Kleinstiver, B. P. Unconstrained genome targeting... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... SpCas9 and its enhanced variants, eSpCas9 and HypaCas9, are great choices. Check out our SpCas9 containing...Cas9 variant with higher specificity (eSpCas9 and HypaCas9) instead.  Choosing a Cas9 for site-specific modifications... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can...

...(2009): 495-505. PubMed PMID: 19300443. 7. Holic, N., Seye, A.K., Majdoul, S., Martin, S., Merten, O.W...lentiviral infection begins with your cells, both your packaging cells and your target cells. Poor tissue culture...harvest after transfecting with the transfer and packaging plasmids. A benefit of the multiple harvest strategy...benefitting your cells, medium exchanges may also impact the quality of the lentiviral particles you harvest...density, volume, incubation time, and MOI can all impact the success of your lentiviral infection. To ensure...

...Dennys-Rivers C, Huffenberger A, Braun L, Rinaldi F, Wein N, Meyer KC, Solano S, Nguyen K , Lang E, Kaspar AA,...trans-splicing, and protein replacement to reverse the impacts of this disease.  As a non-degenerative, monogenic...

...membrane versus the amount of protein loaded on the gel (n=3). Data is presented as mean ± SEM. Adapted from ...controls only address one type of error that can impact your western blot, and depending too much on normalization...

...likelihood of an off-target artifact. Increasing the N in your experiment is the only way to effectively ...in mind, but likely will not have a significant impact on your data. You are trying to generate a knockout...depending on your platform, off-target effects should impact your experimental design differently. Yes, it is...optimization which have led to more specific variants. HypaCas9, sSpCas9(1.1), SpCas9HF1, and evoCas9 are a few...

... https://doi.org/10.1242/dmm.002733 Idalia, V.-M. N., & Bernardo, F. (2017). Escherichia coli as a Model...more model organisms that have left a historical impact on the scientific community. (And, check out this...

...10.1038/onc.2014.470 Taghbalout A, Du M, Jillette N, et al (2019) Enhanced CRISPR-based DNA demethylation...modifications to DNA or histones, the proteins that help package DNA, were associated with gene expression or silencing...particularly large protein, and this fusion allows for packaging into viral vectors and targeted delivery into ...

...vivo. Find MitoTRACER plasmids here! Borcherding, N., & Brestoff, J. R. (2023). The power and potential...Plus, the tools used here (also available as AAV Packaged on Request) will be useful for related studies...transfer increases the metabolic and metastatic capacity of cancer cells. This is certainly just the beginning...

...biocomputing and biological circuits.     References 1. N. Roquet et al., Synthetic recombinase-based state ...are composed. Fear not – the state machine design space is highly degenerate and often times there are multiple... et al. "Permanent genetic memory with> 1-byte capacity." Nature methods 11.12 (2014): 1261-1266. PubMed...

...avoid target sites that code for amino acids near the N’ terminus of the protein, in order to mitigate the...:{"color":"#0052a9","opacity":100},"text_hover_color":{"color":"#ffffff","opacity":100},"button_hover_...button_hover_color":{"color":"#0052a9","opacity":75}}{% endraw %}{% end_module_attribute %}{% module_attribute "tag...thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes Cas9 (SpCas9.../doi.org/10.1038/s41587-020-0561-9 Dempster JM, Pacini C, Pantel S, Behan FM, Green T, Krill-Burger J,...

...Williams P, Zolotukhin S, Reier PJ, Mandel RJ, Muzyczka N (2004) Recombinant AAV Viral Vectors Pseudotyped with...recombination between homologous pairs before viral packaging even begins. This spontaneous recombination persists...2020). In general, we observe that 0.1 - 0.8% of packaged viral genomes contain a recombined/functional ...which can lead to off-target expression of the packaged transgene in neurons and glia.   Check the recombination...v14715, titer 2.4x10e13 GC/mL) for this study were packaged at Addgene and had a relatively low recombination...

...transposons-the-jumping-genes-518/   Sandoval-Villegas N, Nurieva W, Amberger M, Ivics Z (2021) Contemporary...between the transposon’s LTRs, similar to when packaging viral vectors.   Let’s take a closer look at three...after cleavage by the transposase. It has a cargo capacity >100 kB, although integration efficiency decreases...without decreased efficiency, with a maximum cargo capacity of ~200 kB of DNA. Similar to piggyBac, Tol2 also...

... Lisnic VJ, Kremer L, Li D, Koch-Nolte F, Pascual N, Rodríguez-Barbosa J-I, Torensma R, Turley H, Pulford...specificity. However, there are countless variables that impact an antibody’s affinity and specificity for a given...

...The pCoofy series of plasmids contain a variety of N- and C-terminal tags (including His, S-tag, OneStrep...types of pre-cloned insert modules (plant promoter, N-terminal tag, coding sequence of the gene of interest...optimal scaffold can depend on the length of the spacer region between the target sequences. These updates...

...drop-down menu to indicate whether the tag is on the N or C terminus and whether it was present in the backbone...with a unique Deposit ID. Addgene will send you a package containing instructions on how to prepare your ...