... mouse strains. PHP.B plasmids and PHP.eB viral preps are available from Addgene. References Holmes,...

...sent from depositors, bacterial glycerol stocks, prepped DNA ready for our quality control processes - all...The lab prepares bacterial glycerol stocks on Fridays. From each overnight culture, we prepare three sets...sample is barcoded, the lab team takes over and prepares the sample for entry into Addgene’s repository...is contaminated. We use filtered pipet tips and prepare fresh media with antibiotics at each stage. The...Addgene Get help with plasmid transformation, preparation, and analysis from our protocols and molecular...

...! Now, let’s head west, intrepid reader! Protein Prep The first step in any western blot is to lyse your...minutes) at 37 °C, if you suspect aggregation in your prep. If your proteins are the wrong size on your gel...

...that temperature-sensitive materials like viral preps and libraries were kept at the appropriate temperature...

...some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...

...Taylor et al., 2017). However, because purified AAV preps contain similar and low levels of background protein...vector preparations. Addgene has used this method to generally validate our viral vector preparation protocol...al., 2010). The specific infectivity of viral preparations is defined by the ratio of physical viral particles...viral capsids in representative viral vector preparations are genome-containing (Figure 1).    ...Electron micrograph of an Addgene AAV viral vector preparation after negative staining. Empty vector particles...viral service, we currently titrate AAV vector preparations by qPCR. To ensure accurate and reliable results...

...appropriate for a CRISPR-based approach. Second, the AAV preps generally used for gene editing are low titre, and...

...product (see figure). Template A high purity plasmid prep significantly increases the success rate of site...’ll want to steer clear of these strains when preparing the plasmid template for site directed mutagenesis...

... auxillary plasmids may be isolated during the prepping process. How does inducible expression work? As...) General protein expression  m15 pREP4*, ** Kanamycin (pREP4) Cis-repression of the E. coli T5 promoter...similar), inducible under IPTG (lac repressor on the pREP4 plasmid). Expression vector cannot have p15A origin...the strain. Conversely, other strains such as M15(pREP4) use a lac repressor to act directly on the expression...

...recombine. This strain is endA+, so column-based DNA preps require an additional wash step. F- glnV44 recA13...cloning, routine cloning, cloning unstable DNA, preparing unmethylated DNA, and more. Many mutations that...Consequence dam DNA adenine methylase mutation (GATC) Preparing unmethylated DNA, important when trying to cut... dcm DNA cytosine methylase mutation (CCWGG) Preparing unmethylated DNA, important when trying to cut...restriction enzymes to cut the plasmid after preparation. rpsL thr leu thi lacY galK galT ara tonA tsx...

...some of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles...

...out the Addgene AAV guide Find ready-to-use AAV preps from Addgene's viral service ...

...as a kit from Addgene.   Find Ready-to-Use Viral Preps for Epigenetic Modification Why use epigenetic modifiers...

...can vary "Lab assistants" usually wash glassware, prep media, etc. "Research assistants" are more involved...

...removes, not just reduces, empty viral capsids in your prep can help reduce the risk of an immune response. ...LY6C1) Stanton et al., 2023 * indicates viral preps available. Mechanisms of crossing the BBB are indicated...

...Resources on Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...intracellular transport machinery. Addgene offers viral preparations of AAV2-retro vectors with a variety of transgene...

...confirm the identity and serotype of our AAV preparations. We recently published a description of the ... For labs that produce a small number of AAV preparations this level of quality control is sufficient ...facilities producing a large number of viral preparations in parallel, there is a very real risk of mixing...AAVs and the TA-based ligation and tagmentation preparation approaches described previously require a preliminary...effective substrates for tagmentation-based library preparation procedures. This finding eliminates the double-stranding...simplified approach, we sequenced over 100 AAV vector preparations and showed that VGS can quickly and reliably... script that can confirm the serotype of AAV preparations.  Using viral genome sequencing to determine...

...first scientific poster, but unsure how best to prepare. Maybe you’re a presentation veteran, but have ...experience. No matter the reason for attending, your preparation and day-of game plan will ensure you have the...experience possible. Tips for poster session presenters Prepare for success You’ve put a great deal of thought ...it’s time to expertly show off your science.  Prepare the night before: To avoid rushing out the door...poster as a visual aid, not a crutch. Even the most prepared of us can find ourselves talking to our posters... part of a poster session, but with a little preparation and a few deep breaths the day-of, you’ll handle...Strategically traverse the sea of posters If you come prepared with a list of posters of interest, then you’ve...

...reference: OpenWetWare Preparing chemically competent cells (protocol)  Preparing electrocompetent cells... Untergasser Lab Preparation of chemically competent cells (protocol) Preparation of electrocompetent...Research to make competent cells because cells prepared using this kit can be transformed without heat...of electrocompetent cells (protocol)  Cells prepared using the Z-competent TM buffer can be easily ...

...talk to the occasion Don’t just plan to reuse a prepared talk unless it perfectly fits the occasion.  I...figure out how to make it suspenseful. 4. Start preparing your talk early - like today Don’t wait until ...for poor quality slides with sufficient time to prepare (and it does take time). For important talks, ask...loves a great animation. 8. Predict questions, prepare answers Speaking pros always have a few extra slides...come up with clear and concise answers. This preparation will help you avoid giving only yes or no answers...their phones. Speaking longer just makes you seem unprepared or arrogant. A bit short is always better than...