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We narrowed to 577 results for: SIM

Showing: 101 - 120 of 577 results

  1. AAV Packaged on Request is Here!

    Type
    Blog Post
    Published
    Jan. 10, 2025, 1:01 p.m.
    .... To start the AAV packaged on request process, simply navigate to your plasmid of interest. If it’s eligible...cargo you requested at the quality you expect. Similar to our on-demand viral service, we expect titers...There are no quotes or special processes involved; simply request your plasmid of interest in your desired...

  2. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ... used as the first letter of a plasmid name and simply denotes that the object is a ‘plasmid’. “p” is ... empty backbone name in your plasmid name. This simple piece of information can often convey many important...pBACKBONE-Flag-hGene-EGFP or pBACKBONE-Flag-hGene(wt)-EGFP These simple rules allow any scientist to know what is in a ...

  3. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...edited strand. The first iteration of prime editor simply fused the wild-type Moloney Murine Leukemia Virus...or exceed PEmax editing efficiencies for short, simple edits. PE6c and PE6d further evolved the Tf1 and...pegRNAs, but combining PE7 with epegRNAs resulted in similar or reduced editing efficiencies, likely because...Ravisankar, P., Ward, C. C., Levesque, S., Jing, Y., Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt...

  4. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...Interspaced Short Palindromic Repeats. That contrast of simplicity and complexity is reflected in the biology, too...into a single strand that folds back on itself, simplifying the CRISPR-Cas9 system for laboratory applications...post on the structural biology of Cas9. Want a simpler guide to keep at your desk? We’ve got a CRISPR ...

  5. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ... the Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your STR profiles ...CLASTR results by minimal number of markers and similarity score match. This tool’s algorithm can be adjusted...

  6. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...control over neuronal activity. While they serve similar purposes, both techniques offer researchers different...resolution as receptors can be turned on and off simply with the presence or absence of the correct wavelength...flexible as it relies on designer ligands that can be simply introduced by injection. The main challenge in ...

  7. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

    Type
    Blog Post
    Published
    Feb. 13, 2018, 2:51 p.m.
    ...and cell lines. Although ChIP-seq is a relatively simple and robust protocol it does have limitations. The...change offers a significant improvement and simplification of the original technique, specifically in ...CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids for isolating...

  8. Sensing Neuronal Dopamine

    Type
    Blog Post
    Published
    July 30, 2019, 12:59 p.m.
    ... DA in live animals (Patriarchi et al., 2018). Similar to calcium and glutamate sensors, the new dLight1...s lab has created the GRABDA sensor based on a similar strategy: a GFP variant bound to a DA receptor,...cyclase in caudate nucleus of rat brain, and its similarity to the “dopamine receptor”." Proceedings of the...

  10. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...ve ever wondered how they are different from or similar to the other Ts, we have your answers. If you know...into a cell by a viral agent. Transductions are similar to transfections, processes which introduce foreign... Transfection and transduction seem to achieve similar outcomes, so why and when should you opt to make...

  11. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...multifunctional fibers, embedded, and validated simultaneously in the brain and the intestines of mice. The...organisms, including the Anikeeva lab’s work on a similar neural fiber (Park et al., 2019). This provided... To operate both types of implanted fibers simultaneously, a mini-Bluetooth system named NeuroStack was...

  12. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...Viruses are simple: a genome packaged in a protein shell (Taylor, 2014). They’re so simple that we can... even decide if they’re alive or not. Yet these simple, small particles have quite the outsized impact...

  13. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ...equipment. Reporters for studying gene expression have similar drawbacks, often requiring the destruction of plants... events and am currently planning to use it to simplify my work with an Arabidopsis mutant that can’t ...unfamiliar with these mutant plants. Using RUBY to simplify the identification of these mutants will speed...

  16. Single-cell tracking of lineage and identity with CellTag

    Type
    Blog Post
    Published
    May 9, 2019, 1:15 p.m.
    ...provides a combinatorial indexing strategy that simultaneously tracks clonal history and cell identity at ...that cells with common ancestors tended to have similar reprogramming outcomes. For example, cells with...with shared CellTagD13 tags often had similar gene expression pattern changes and reprogramming outcomes...

  17. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ... multiple sgRNA constructs to be introduced simultaneously in a single plasmid. The functionality of the...of S. cerevisiae and E. coli. The utility of simultaneous and multidirectional multiplex control via scRNA...Multiple scaffold RNAs can be designed to allow simultaneous, multidirectional and tunable control of gene...

  18. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...levels for 6 days - after just 15 minutes of FUS.  Similar constructs to the one above can be made for expressing...But why might you need a second GFP antibody?  Similarities:  Generated against full length GFP and converted...the group rationally designed GFP variants with similar capabilities. If you’re looking for a super stable...

  19. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

    Type
    Blog Post
    Published
    July 21, 2017, 12:48 p.m.
    ...the Gal4/UAS system. This incredibly useful, yet simple system allows you to systematically study gene ...downstream of a tissue-specific promoter sounds simpler, this modular system saves you from having to create...compartment, you could express them individually or simultaneously. Do note for control purposes that there are...

  20. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ...disappears from the video, and prepares a coffee, or simply moves away from the desk to switch off a few minutes...GA, O’Reilly CM, Lofton ME, Catalán N (2018) Ten simple rules for collaboratively writing a multi-authored...journal.pcbi.1006508 Vicens Q, Bourne PE (2007) Ten Simple Rules for a Successful Collaboration. PLoS Comput...
Showing: 101 - 120 of 577 results