Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 815 results for: ache

Showing: 101 - 120 of 815 results

  1. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ... viral vectors, and high-throughput screening approaches. Here, we’ll highlight these recent advancements...copies of the p65-HSF1 transcriptional activator to each genome-bound dCas9 (see Figure 1a for the transgenic... a larger packaging capacity than AAV. Such an approach was described recently by the Day Lab, who developed...different untranslated or coding regions. Typically, each gene isoform has a distinct transcriptional start...least, more straightforward than generating cDNA for each gene isoform individually. To highlight this advantage...protein. To demonstrate the specificity that can be achieved with CRISPRa, the Day lab singled out two Bdnf... developed a subgenome-scale CRISPRa screening approach in embryonic stem (ES) cells to determine which...

  2. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    Published
    May 8, 2018, 1 p.m.
    ...then characterizes the sequence and abundance of each particular indel.   For a complete guide to ICE ... to gather details on the issues associated with each sample. Successfully analyzed samples will display...Sample - The unique label name that you provided for each sample. Guide Target - This is the user-defined ... name. You can perform more in-depth analyses on each sample by clicking on the sample name or on its ...analysis will open up a new window with three tabs.  Each of the three tabs - Contributions, Indel Distribution...entire edited population of genomes. Hovering over each bar of the indel plot shows the size of the insertion...base calls from the .ab1 file are also shown above each trace. The horizontal black underlined region represents...

  3. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    Published
    March 7, 2019, 2:39 p.m.
    ...cytometers. These fluidic machines handle biological samples and are cleaned between each use. You wouldn’t throw...New ideas and approaches are encouraged in science. It isn’t surprising that new ideas are also popping...lab lives. Read on to learn about some creative approaches to lab sustainability, and find ways to further... throw away your FACS machine after a single use. So how about washing and reusing pipette tips in a high-throughput...tips. This is part of a cultural change in how we approach waste that needs to be supported at all levels...industrial and residential markets. Rethinking our approach to lab waste is something we all need to do. Remember...

  4. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    Published
    Jan. 10, 2019, 2:38 p.m.
    ...’s very sad because it’s years of work,” Sacher says. Sacher hopes that as Phage Directory expands, it...When Jessica Sacher, a microbiologist from the University of Alberta, saw that scientists were using ...recognize a specific part on the bacterium making each phage is very specific. Some infect only one or ...really needs it, but then it might to be too late,” Sacher says. As the labs in the directory step forward...Since the inception of Phage Directory ~1 year ago, Sacher and Zheng found that they could expand their role...information and potential for successful phage therapy, Sacher and Zheng also saw the need to archive phage reagents...

  5. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...and flexibility to the CRISPR editing world. The approach, originally published in Nature, is a “search-... sequence location The prime editor extends the reach of CRISPR genome editing, as it can edit near to...prime editing typically needs to be optimized for each application, and the efficacy varies widely — it...versatile, but it requires careful optimization to achieve the best efficiency. The type of edit, genomic ...strand (Chen et al., 2021). The team applied this approach to both the PE2 and PE3 systems, naming the new...optimized enzyme can be used with any of the PE2-5 approaches, sometimes referred to as PE2max, PE3max, etc...improved efficiency, although both enzymes still approach or exceed PEmax editing efficiencies for short...

  6. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...   Many thanks to our guest blogger, Joachim Goedhart! Joachim Goedhart is an assistant professor at ...You can follow him on twitter: @joachimgoedhart.   References 1. Zacharias, David A., et al. "Partitioning...This post was contributed by guest blogger Joachim Goedart, an assistant professor at the Section of ...generate the strictly monomeric EGFP variant, mEGFP (Zacharias et al., 2002). GFP needs oxygen Chromophore formation...protein module that can report on location. This approach enables the study of proteins in cells, but not...tolerated in my fusion It is standard practice to attach the GFP to the N- or C-terminus of another protein...

  7. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...intellectual journey. Practical approaches One approach to achieve this goal could involve participating...moments of release and mental freedom. Practical approaches Engaging in mindful meditation, taking field ...or visiting museums can be excellent methods to detach from the constant stream of thoughts related to...predefined path, opening doors to novel ideas and approaches. Moreover, the ability to embrace serendipity....” This quote encourages us to contemplate our approach to life and how our viewpoints influence our encounters...

  8. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ...live across the country or around the world from each other are collaborating on projects. When I was ...experiments can get done and data analyzed between each meeting, but short enough that everyone stays on...meet. You and your collaborators should then hold each other accountable to these expectations once they...checking in about them at your recurring meetings and reaching out if any milestones are missed. Spend time in...think about having collaborators spend time with each other in person. One possibility is to do a lab ...lab exchange, where researchers spent time in each other’s labs. This is an opportunity for the researchers...researchers involved to learn techniques from each other, access specialized equipment, or ensure that techniques...

  9. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...experiment. Nucleic acid based machines Recombinase-based State Machines Enable Order-dependent Logic in... cell. These so-called recombinase-based state machines could potentially be used to report upon and/or...post to learn more about recombinase-based state machines and how they function. Adapting Toehold Switches...Cressy from the labs of Dr. Elke Mühlberger and Dr. Rachel Fearns at Boston University School of Medicine,... describes R-bodies, “force generating protein machines that can extend and retract over many microns ...you can better design your donor DNA template to achieve more efficient genome editing - all influenced ...

  10. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...more progress you will make in reaching them. Of course, you won’t reach all the goals you set, but the...bring more than their knowledge and teaching to help a Mentee reach goals. One of the most important contributions...relationships, so that you can reap rewards in the form of reaching career development goals. Check out Joanne's Reddit... adjust expectations, and record milestones in reaching goals. The more time and thought you put into ...being a Mentor: Learn other perspectives Develop coaching skills Encourage your creative thinking Enhance...

  11. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...with greater spatial and temporal control than is achieved by traditional pharmacologic methods. A number...covalently linked molecules (i.e. photoswitches) attached near the binding pocket of an engineered receptor...PORTLs use a similar concept, except the ligand is attached to a protein binding partner or nanobody that ...secondary signaling pathways with light. This is achieved by coupling endogenous signaling molecules with...the activation curves (Marshel et al., 2019) for each tool, and you may have to test and optimize the ...sources used to prevent activation of both tools with each light source used.   Channel and activation kinetics...it is important to understand the details of how each tool performs and the consequences of off-target...

  12. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    Published
    Feb. 9, 2021, 2:15 p.m.
    ... of each talk - time for free interaction and brainstorming. This could be achieved by asking each presenter...leadership, and inappropriate tools or writing approaches can lead to frustration, delay of publication... someone could raise their hands or signal the approach of a brain break, where everyone stands up, disappears...general chatter will be allowed with a different host each time that moderates conversations. This can help...social distancing. The take home message is the approaches/tools we have to foster creative collaboration...

  13. How to Lead a Great Meeting

    Type
    Blog Post
    Published
    Feb. 9, 2016, 3:30 p.m.
    ... the topic at hand. Invite one “ambassador” from each affected team to be the representative in the meeting...standing team meetings, provide agenda sections for each person to add a bullet or two about what they are...many points as they like to the written agenda, but each only gets to address one point aloud. This keeps...separate section at the top of the agenda, and assign each one using group members’ initials. If appropriate...Consider whether you actually need a full hour to achieve the purpose of the meeting (see “defining the scope... and are able to provide more timely feedback on each other’s projects. For all-company meetings, monthly...still works best. Figure out what works for you for each of your meeting types (see above). We’ve provided...

  14. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...revolutionary physical approaches. However, with each new technical achievement, the complexity of the...disciplines. In biology, innovative new imaging approaches are constantly being developed which combine ...with the community are particularly successful. Machine-learning denoising of raw data (CARE), segmentation... greatly increases accessibility. Through this approach, the Planktoscope, a portable device for studying...and Deployment." bioRxiv. 2020. Diederich, B., Lachmann, R., Carlstedt, S. et al. A versatile and customizable...

  15. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...this need in the community, we approached it in the way Addgene approaches everything; we asked ourselves...some of the people and organizations who helped us reach this milestone. Viral service supporters We would...from laboratories all over the world. With this reach and exposure in the community, we saw the opportunity...shipping systems and protocols for viral vectors to reach scientists around the world. Our Software Engineering...our systems for this new type of material. The Outreach Team is getting the word out about this new resource...preps derived from plasmids in the repository. The Outreach Team has been making sure scientists know about...

  16. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    Published
    Feb. 2, 2021, 2:15 p.m.
    ... any eligibility provision, don’t guess. Simply reach out to the program coordinator with your question...best to draft a cover letter that is customized for each internship. Lastly, have your resume and cover letter...statement.  4. Letters of recommendation wisdom  Reach out to 2-3 individuals in your professional circle...opportunity for themselves. This is ultimately the wrong approach. It is very likely you know individuals in your...cohort. This may sound counterintuitive since that approach seems to create competition for your own spot.... Pasteur These tips are a set of common sense approaches that will prepare you for the competitive landscape...Hospital of Braintree. Rose is passionate about outreach among low-income communities to educate parents...

  17. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ...overview). Until recently, the approach has been hampered by the need to tag each protein of interest and, ...Proximity-dependent labeling has become a powerful approach for proteomic mapping in both cell culture and...provides researchers better flexibility and makes the approach more accessible, as it reduces the need to generate... barcodes for live cell clonal multiplexing by: Rachel Leeson Multiplexed images in live cells remains...multiplexing of cells in live imaging application. Each visual barcode consists of a fluorescent protein...four different fluorescent proteins, and inserting each visual barcode into its own subclone, they were ...

  18. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ... of each other. That is, multiple independent variables are assumed to not have any effect on each other...variables are affecting each other – or if you think they may be affecting each other – then a MANCOVA ...variance, data ranges. how closely data points fall to each other (see Fig. 1). Alpha values – numerical ... if only two of those samples are different from each other, or if every permutation of pairs within the...null hypotheses. Benjamini uses a complementary approach which identifies a p value cut off. This method...Duncan’s multiple range test, and several others. Each test has different likelihoods of returning false...

  19. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...ions crosses the cell membrane, a threshold is reached and the neuron fires, sending the electrical signal...specificity far greater than the one that can be achieved using pharmacological or electrophysiological ...., 2016; Hudry and Vandenberghe, 2019). How to achieve strong opsin expression in specific neuronal cell...expression in vivo (Schnütgen et al., 2003). This is achieved through site-specific recombinases such as Cre...expression in specific neuronal cells? This can be achieved using Cre-dependent viruses, such as the optogenetic...love for educational science led her to pursue a teaching career, and she now is a very happy scientific...Philippe Coulon, and Ilka Diester. "Optogenetic approaches to study the mammalian brain." Current opinion...

  20. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    Published
    March 3, 2020, 2:15 p.m.
    ... identical in sequence to the unmodified enzyme. Each half of the base editor is fused to a fast-splicing...GFP. The lab tested the base editors in vivo and achieved therapeutically relevant editing efficiencies ...heart, and skeletal muscle. The dosing required to achieve these efficiencies was also between 1013 - 1014...organisms such as E. coli and yeast. Because this approach uses standardized parts, it allows for greater...controlled by red light by splitting Cre and fusing each half with PhyB or PIF6, phytochrome-like fusion ...prosthetic group PCB, PhyB and PIF6 dimerize, bringing each terminus of Cre together to restore Cre activity...fluorescent proteins controlled by Cre recombination. Each cell can have multiple copies of the transgene that...
Showing: 101 - 120 of 815 results