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Showing: 101 - 120 of 292 results

  1. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    Published
    Nov. 14, 2024, 2:15 p.m.
    ...polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.    Alternative ...Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial Immunity. Science. 2012;337(6096):816...

  2. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ...routinely in biotechnology to transform plant cells. Degradative plasmids: microbial hardiness There are also ...organisms. To conserve energy, these specialized degradative capabilities are often tightly regulated to be...

  3. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...include a lot more. Font size: Making your poster readable While we are talking about font, remember this...this: bigger is better! Your poster should be readable from several feet away. It can also be useful to ...

  4. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ... the simplest CRISPR systems and the easiest to adapt to genome editing, but the introduction of type ...positions are indicated below each graph. Figure adapted from Kleinstiver et al., 2016. Until recently...

  5. Plasmids 101: Terminators and PolyA signals

    Type
    Blog Post
    Published
    March 31, 2016, 2:30 p.m.
    ...longevity in eukaryotes and promote transcript degradation in prokaryotes.  Prokaryotic termination   Prokaryotic...important for stability of the mRNA, protection from degradation, and is integral to the nuclear export and translation...

  6. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...interference (CRISPRi) has been limited to lab-adapted bacteria because it has been challenging to introduce...for using CRISPRi in many types of bacteria: lab-adapted, environmental isolated, and disease-associated...

  7. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ...References and Resources References Braun, C. J., Adames, A. C., Saur, D., & Rad, R. (2022). Tutorial: design...Teyssier, N., Pan, A., Danner-Bocks, S., Sanghal, T., Yadanar, L., Tian, R., Ma, K., Palop, J. J., & Kampmann...

  8. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic tools, they’re optimistic...optimistic that MagLOV can be similarly adaptable into future magnetogenetic tools.  Embracing science...

  9. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...the kinetic response time from signal input to readable output, which generally exceeds that of the VSD...of the previously described systems. Several adaptations of this method have been to tag, cage, uncage...

  10. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... co-purify with your plasmid DNA and lead to degradation of your prep. Many plasmid prep kits recommend...strains are not good for cloning Bacterial strains adapted for protein purification use the cell’s resources...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...disadvantages of the IRES element, scientists have adapted "self-cleaving" 2A peptides into their muliticistronic...blog posts and additional resources into one downloadable PDF that you can save on your desktop. The Plasmids...

  12. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...or TE. The Bishop Lab’s glass milk protocol is adapted from the 1979 Patterson Protocol. When published...100 bp to 48 kb with high yields and without degradation during the extraction procedure. Additionally...

  13. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...resistance to both carb and amp, due to their common degradation mechanism. How to choose between the two? Carb...high pH environments and is less susceptible to degradation in agar plates. This prevents satellite colonies...

  14. An Introduction to Adenovirus

    Type
    Blog Post
    Published
    Oct. 3, 2023, 1:15 p.m.
    ...cellular events, all recruit strong innate and adaptive immune responses that inactivate viral genomes..., J. S., Lovett, S. P., Parker, K., Radune, D., Hatada, A., Pan, C. Y., Rizzo, K., Bertumen, J. B., Morales...

  15. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    Published
    Sept. 6, 2022, 1:15 p.m.
    ...Brans, M. A. D., Luijendijk, M. C., Garner, K. M., & Adan, R. A. H. (2010). Optimization of Adeno-Associated..., Malaker, S. A., Poweleit, N., Maynard, L. H., Adams, C. M., Leib, R. D., Cirolia, G., Thomas, D., Stamnes...

  16. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    Published
    Dec. 7, 2020, 2:53 p.m.
    ...spreading within and between genomes, and have been adapted for a wide variety of applications - as mutagenesis...at two different incubation temperatures. Data adapted from Vo et al., 2020. In E. coli, the new streamlined...

  18. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon...
Showing: 101 - 120 of 292 results