...This post was contributed by Future of Research Executive Director, Gary McDowell. On Dec 1st, the threshold...How did this change in labor laws come about? In March 2014, the White House issued a memorandum to the...issued by the Department of Labor. At Future of Research, our volunteers have been helping to gather information... to the National Institutes of Health National Research Service Award (NRSA) stipend levels. In theory...workforce. Based on our communications with and research on these institutions, we can say with relative...climate combined with the lack of a sustainable research enterprise, there is no easy way for academia ...enterprise in the U.S. will decrease. At Future of Research, we think this is a good thing. Reliance on a ...

...is a component of the immune system useful as a research reagent? Let’s find out! What is an antibody? ...system and a tool that can be used for a variety of research applications. Within the immune system, antibodies...ability of antibodies to bind proteins is useful for research applications as well because they allow scientists...and initiates different immune cascades. In the research setting, antibodies of different isotypes can ...antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always going to spot the whole...string of letters is a part of the word you’re searching for. Antibodies may recognize a linearized sequence... many ways that antibodies can be produced for research purposes, some of which take advantage of natural...

...fluorescent proteins in their research. These awards help early career researchers attend a conference of their...opened up applications to research technicians and undergraduate researchers, in addition to postdocs ...postbaccalaureate research technician in Kaspar Podgorski’s lab at Janelia Research Campus, HHMI where.... Below, read about the five winners and their research.   Abhi Aggarwal: Glutamate sensing reporters... Pedre Pérez is a postdoc at the German Cancer Research Center (DKFZ) who has developed new redox sensitive...that detect new redox metabolites are vital for researchers to better understand the processes that happen...University in the department of Physiology. Her research focuses on the neural circuitry involved in mechanical...

...Get on that barcode gravy train Buy a barcode label printer. Buy barcode scanners. Barcode everything....another. If you are using barcodes (and you should be!) bear in mind that your barcodes are being applied to...everything. If it’s barcoded, it should have a corresponding entry in your database. Enjoy your happy lab staff...staff. Barcoded materials make everyone's life easier, the time and capital cost of getting up to speed...containers, not to the contents. Tightly coupling barcodes and containers might not be such a bad idea, tightly...Resources on the Addgene Blog Learn about How We Use Barcodes in the Lab Get Tips on How to Keep a Lab Notebook...Resources on Addgene.org Find Plasmids for Your Research ...

...consider while writing each section. Research challenge The Research Challenge motivates the request for...lead to new milestones in the field. Research strategy The Research Strategy highlights i) the significance...Research at universities typically requires funding from a variety of government and academic institutions...overview information, key dates, funding description, research plan, award information, eligibility information...aims requested in the call. Step 2: Develop a research plan Most grant applications restrict the space... and ii) how you, the expert, will address the research challenge. Now is the time to make bold statements...that your solution is the best approach to the research challenge. Demonstrating expertise in the field...

...to expect: Search bar The biggest change is to our most frequently used feature: the search bar. We’ve... now has two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is... the search box to go straight to your materials of interest!   Figure 1: The new search bar.   Pro tip...is our same search bar. And if you’re in the mood to browse our catalog, you can use the links under the... tip! You can use a manuscript title to search for plasmids from a specific paper. If you’re ready to ...deposit, simply click on “Deposit Materials” and the search bar will change to an easy-to-use depositing guide...common activities can be done straight from the new search bar, but the menu just above it can help you quickly...

... by guest blogger Greg Lohman, a biochemistry researcher at New England Biolabs. When do you need a high...high-throughput profiling New England Biolabs® (NEB) researchers recently published a method for the high-throughput...blogger, Greg Lohman! Greg Lohman is a biochemistry researcher at New England Biolabs, specializing in the enzymology...Thermus thermophilus DNA ligase." Nucleic acids research 24.15 (1996): 3079-3085. PubMed PMID: 8760897....in duplex DNA by T4 DNA ligase." Nucleic acids research 10.5 (1982): 1425-1437. PubMed PMID: 7041091. ...can be sealed by T4 DNA ligase." Nucleic acids research 15.21 (1987): 8755-8771. PubMed PMID: 3684572....mononucleotide repeat sequences." Nucleic acids research 27.24 (1999): e40-e47. PubMed PMID: 10572192. ...

...idea of the themes in the field, I searched for recent research papers on these particular themes, for... your ability to write, to read efficiently, to search the literature, and to synthesize a large volume...particular topic. Alternatively, maybe you’re researching a topic for which no relevant or recent review... the field you plan to review. If it’s a well-researched field, you may need to get specific to make sure... has the benefit of exposing you to relevant researchers in the field. Finally, you can sound credible...an expert in the field. To find reviews, I just searched online and found ones that I thought “looked good...cared about. I also used reviews to get a list of research papers that I needed to read. Once I had an idea...

...distribution of plasmids for COVID-19 research is accelerating essential research in laboratories all around the...sometimes wonder if we can take another blow. Back in March, we never expected to be in this here and now. Our...Here's the message I shared with the Addgenies on March 11, 2020 after rumors spread in our building that...scientists around the world mobilized on COVID-19 research, cures, and diagnostics. Almost immediately we...proud of the work we’ve done to support COVID-19 research. Our collection of available materials is growing...the crucial role Addgene plays in accelerating research. Yet this was still not enough to ensure we could... doing almost every type of biomedical science research. Our work accelerates and enhances productive ...

...individuals whose research ranged from wildlife conservation to abortion law to poetry. A research discipline...not ultimately translate to a desire to do the research itself. Several years of exclusively writing lab...depicting nuclear fission; a podcast with a genetics researcher on parasitoid wasps; and an essay on the life...versatility in the face of disparate spheres of research. In my case, an interest in neuroscience was carried...occupied my attention, until these coalesced into a research topic on concussion in sport and the public’s ...sport my entire life, I was curious as to what the research had to say. Moreover, this subject allowed me ...public utilizes insights gleaned from scientific research is, after all, one of the enduring goals of science...

...and an inability to improve upon these critical research tools. Addgene is excited to announce that we ...problem.The grant, which is part of the NIH’s Brain Research through Advancing Innovative Neurotechnologies...high-quality antibodies/nanobodies for neuroscience research and is an expert at validating these tools. While...offer numerous advantages, including more secure archiving, unambiguous molecular definition, and potential...the most commonly used reagents in neuroscience research today, but there is a critical unmet need for ...financially self-sustaining through distributions of research materials, such as plasmids and viral vectors....acquisition of affinity reagents and will enable researchers everywhere.” The timing is ideal as the NIH’...

... Overview of the CRISPR classification hierarchy. The hierarchy flows from Class to type to subtype to...a decade of frenzied interest in bacterial and archaeal immune systems. During this time, scientists discovered...perhaps provide insights and ideas for your own research! Classification Structure Principles of CRISPR...fast evolution of these adaptive bacterial and archaeal immune systems has led to extreme sequence divergence...classifications were placed into a fairly simple hierarchy: class to type to subtype to variant. Despite ...seemingly complicated steps to arrive at this hierarchy, the main aspects of each class and type are easy...identified CRISPR-Cas in bacteria and nearly 100% in archaea, though Class 1 remains largely underutilized compared...

...proteins have enabled scientists to pursue creative research avenues previously unavailable to them. With these...interactions. Beyond these common applications, researchers are finding new ways to apply fluorescent proteins...Roger Tsien played enormous roles in enabling researchers to utilize and develop these tools by making ...fluorescent protein technologies widely available to the research community. To honor their legacy, we recently ...help scientists share their fluorescent protein research with the academic community by funding their travel...demonstrated the utility of these FLiPs in a variety of research applications and particularly notes their ability...inducible enzyme technologies. She hopes that other researchers will be able to use similar designs to create...

...publication.   Do research! Nearly everything we write entails a primary literature search to collect up ...webinars, or use other research methods. In the case of the antibody guide, primary research and previously ...easily and quickly access tools to further their research. However, tools aren’t very useful if you don...goal is to provide informative content to help researchers in creating and sharing knowledge, related to... types of materials we help distribute and the research communities that we serve. How we curate (and...you didn’t know you wanted to know about, like research breakthroughs or cool new tools. And while this... Center, help.addgene.org, where you can easily search articles answering all of our frequently asked ...

...Addgene.org Find plasmids for your research Find cancer research plasmids and resources ...represents a considerable barrier to furthering research and developing effective treatments for the disease...work, and how these plasmids can help cancer researchers studying an assortment of genes. Using patient...Anderson Cancer Center patient databases, this research team identified 1,049 perturbations in several...the High-Throughput Mutagenesis and Molecular Barcoding (HiTMMoB) technique (Tsang et al., 2016), creating...functional annotation of thousands of mutations The researchers used the collection to screen individual perturbations... platform is an incredible boost to the cancer research community, but what else are these plasmids useful...

...mission: Accelerate research and discovery by improving access to useful research materials and information...which we promote our mission and work to enable researchers around the world. We take this responsibility... by generating revenue through distribution of research materials. Our small shipping and handling fees...materials shared through Addgene are being used for research purposes and not for a profit motive. We build... we will always look for new ways to serve the research community. Questions? Feel free to reach out to...science and Addgene in our mission to accelerate research with easier sharing and collaboration. Joanne ... Kayla, Kelly, Kory, Kris, Leila, Lianna, Luke, Marcy, Mary, Max, Maya, Meghan, Melina, Meron, Michael...

... the progress of research, they can also present hurdles for the team of researchers involved. Communication... where researchers spent time in each other’s labs. This is an opportunity for the researchers involved...Communication is a major challenge when researchers are communicating across disciplines or doing different...or Google Hangouts is a great way to include researchers from different locations. Having virtual face-to-face...best for them. This also accelerates the pace of research by circumventing the slower, sequential communication...document. Set and manage expectations When the researchers involved in a project are scattered across the...future directions of the project. The state of research is constantly shifting, and long-distance collaborations...

...describe all cell lines used in research, using four methods to search out cell lines: Periodic surveying...approximately one-third of cell lines used in scientific research are misidentified! Rigor and reproducibility of...collections websites and catalogues Literature searches utilizing Google Scholar alerts and the NCBI LitSuggest..., such as their vaccine production, SARS-CoV-2 research, or adenovirus packaging cell lines dataset collections...was described. Using Cellosaurus Cellosaurus is searchable by cell line name (recommended names and synonyms...You can also use the Cellosaurus STR similarity search tool (CLASTR) to find cells lines similar to your...adjusted based on query, reference, and shared allele search preferences to help you identify proximal lines...

...Neurodegeneration Research Collection. This page is regularly updated with useful materials for research into Alzheimer...editor PE7 Antibodies to facilitate SARS-CoV-2 research New viral vector preps of Voltron2 voltage sensors...1: CROPseq-multi uses two sgRNAs with internal barcodes (iBARs), multiplexed using tRNAs, within the lentiviral... team designed sgRNAs that include an internal barcode and separated multiple sgRNAs with tRNAs, which...motif to stabilize the 3’ end of the pegRNA. As research has just begun with PE7, it will be exciting to...38570691. Antibodies to facilitate SARS-CoV-2 research   By Ashley Waldron Ready-to-use recombinant antibodies... the Addgene repository of tools for SAR-CoV-2 research. Two new antibodies, Anti-SARS-CoV-2 Nucleocapsid...

...pAAV.hSyn-FLEX.iGABASnFR.F102G pAAV-Syn-Archon1-KGC-GFP-ER2 pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2] pAAV-FLEX-tdTomato...constitutive (pAAV-Syn-Archon1-KGC-GFP-ER2) and cre-dependent version (pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]) ...iGABASnFR and its F102G variant in AAV1 at Addgene! Archon1, a voltage sensor AAV Historically, electrical ...brain was measured with implanted electrodes. The Archon1 voltage reporter allows a way to monitor neuronal...imaging. Since it’s illuminated by red/orange light, Archon1 can also be used in conjunction with optogenetic...this membrane-embedding voltage detector. Find Archon1 and its cre-dependent version at Addgene! Fluorescent... viral protocols and videos Find AAVs for your research ...