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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 284 results for: cki

Showing: 101 - 120 of 284 results

  1. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...Figure 4). Figure 4: STITCHR consists of a nicking Cas9 fused to a shortened R2Tocc transposon protein...Cas9 nickase to improve target recruitment and nicking, producing STITCHR. STITCHR can make a wide variety...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...searching, DNA synthesis, and plasmid assembly. Luckily, researchers from Douglas Densmore’s lab at Boston...Nucleic Acids Res. 1996. PubMed PMID: 8692687 Tracking autophagy in yeast with Rosella, a pH-sensitive...the actuator’s subcellular localization using trafficking signals and named it Magneto 2.0. Using Magneto2.0...

  3. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...found here. Figure 3: A surprise for the author. Checking Cas9 expression Overview: By Western blotting ...C, Vogelstein B, Bunz F. Genetic knockouts and knockins in human somatic cells. Nature Protocols 2007;...

  4. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...silencing effect through targeted mRNA degradation, blocking translation, or chromatin modifications of complementary...researchers studying plants and other organisms lacking this innate immunity need not worry and have benefited...

  6. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    Published
    March 14, 2017, 2:30 p.m.
    ...pathway, VEGF production, and CTLA4 and CD protein blocking. Other molecular targets under research include...collaborative therapies must be explored. When attacking cancer, multiple immunotherapy targets can be ...

  7. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...more! Engineering disease resistance through gene stacking, increasing production of a protein of interest...requirements and editing abilities, such as base editing, nicking, cleaving, etc. The possibilities really are endless...

  8. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...using the application correctly is imperative. Picking your chemistry  The two most common qPCR chemistries...thermodynamic and experimental parameters (neighbor stacking energies, loop entropy effects, cation concentrations...

  9. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...Addgene we’re getting into the holiday spirit by kicking off our annual #DeckTheLab contest. The bar is ...

  12. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...crafty holiday decor that we will never forget! The Mackin Lab from University of Colorado -Anschutz Medical...

  15. New Videos: Addgene Lab Tips

    Type
    Blog Post
    Published
    Feb. 15, 2018, 2:41 p.m.
    ...keep track of reagents in your lab. With barcode tracking, you will always be able to locate materials quickly...

  16. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...video channels are also underrated and I recommend checking them out, especially since videos on synthetic...

  18. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...powered by 100% renewable electricity! A (not so) shocking transition  In early July the Town of Watertown...

  20. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...with Eye Researcher Connie Cepko Get Advice on Picking a Graduate Lab and Project Check Out 5 Science ...
Showing: 101 - 120 of 284 results