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Showing: 101 - 120 of 238 results

  1. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...have deposited the best performing sensors and constructs encoding corresponding portions of the Zika virus...virus research community. Find the Zika Sensor Constructs at Addgene As we report, we have embedded this...

  2. Introducing Addgene's Microbiology Pages

    Type
    Blog Post
    Published
    June 13, 2016, 7:08 p.m.
    ... free distribution services for their popular constructs, why not let us know? Fill out our referral form...

  4. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...the plasmid name: This is the name given to the construct by the depositing lab. There may be other plasmids... Principal Investigator (PI) in whose lab the construct was made. The depositor name links to a page listing...

  5. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... Addgene’s collection of empty backbones for constructing fluorescent fusions. Transcription Reporter:...at Addgene. Cell marking/selection: Expression constructs like plasmids often include GFP as a marker to...

  6. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...experiments. You may have to design several different constructs to find one that could be used for the purpose...monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular ...

  8. Fueled by Coffee at #SfN14

    Type
    Blog Post
    Published
    Nov. 18, 2014, 7:15 p.m.
    ...labs how they can benefit from depositing their constructs to the repository, and getting feedback from ...

  9. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...Molecular Cell Find the Nme2Cas9 All-in-One AAV constructs CRISPR interference in Candida albicans A new...

  11. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ... of different studies. Elly Tanaka’s lab has constructed several vectors for use in the axolotl including...GreenGate Toolkit which allows for easy cloning of constructs specifically designed for use in Arabidopsis....

  12. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...two-photon illumination than previous GEVIs. Some constructs are also available as viral vectors (here and...and voltage sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-syn-FLEX-...

  15. Editor's Choice, August 2016

    Type
    Blog Post
    Published
    Sept. 2, 2016, 1:32 p.m.
    ... a quick way to generate many mutated plasmid constructs. The second post was contributed by Addgene Scientist...

  16. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...mice pre-CRISPR was very arduous. First, you’d construct a plasmid with your desired changes, long homology...template, you'd use ivTRT to create the ssDNA construct as described in Figure 2.  You can also use Addgene...

  18. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...hypothesis that the lower expression levels of SunTag constructs would avoid negative effects on cell physiology...

  19. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ...and tracking cells that express both the dCas9 construct and the fluorescently-labeled gRNA multiplex. ...
Showing: 101 - 120 of 238 results