Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 282 results for: fus

Showing: 101 - 120 of 282 results

  1. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    Published
    Feb. 16, 2023, 7:05 p.m.
    ...interference (CRISPRi) targets a catalytically dead Cas9 fused to a transcriptional repressor to your gene of interest...activators (CRISPRa) are transcriptional activators fused to a dead Cas9. The system is led, via a custom ...specific ligand to mark the tag and whatever it is fused to for degradation. Since the tagged protein of ...

  2. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type
    Blog Post
    Published
    Nov. 24, 2015, 3:30 p.m.
    ...showed that nanobodies could be fused to other proteins, and these fusions retained the ability to bind ...Caussinus, E., Kanca, O., and Affolter, M. “Fluorescent fusion protein knockout mediated by anti-GFP nanobody....

  3. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...our FRET Resource page and can be used to create fusions with a gene of interest. These plasmids will be..., where the donor and acceptor fluorophores are fused to two separate proteins. (In contrast, intramolecular...achieve, because the ratio of acceptor to donor fusions varies with transfection efficiency and any unpaired...

  4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...protein-protein interactions. One popular approach is to fuse a protein of interest to each part of a split fluorescent...General Design Figure 1: Two proteins are fused to the FP(11) and FP(1-10) fragments. When Protein...cell environments). Also consider that a protein fusion may disrupt functionality or binding of your target...

  5. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...replace” genome editing technique that relies on a fusion enzyme of Cas9 nickase + reverse transcriptase ...Nelson et al., 2022). PE7: This editor includes the fused RNA-binding protein La to protect the end of the... not epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). Figure 9:...

  6. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...with Peptide-Assisted Genome Editing (PAGE). They fused the Cas enzyme to cell-penetrating peptides and ...guides. Figure 1: A) Cell-penetrating Cas9, fused to HIV TAT, Myc and SV40 Nuclear Localization Signals... et al. (2023). StayGold variants for molecular fusion and membrane-targeting applications. Nature Methods...

  7. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type
    Blog Post
    Published
    April 6, 2021, 12:30 p.m.
    ...  Confirming tags and fusion proteins Finally, we confirm promoters, tags, fusion proteins, and selectable...NCBI entry or using BLAST.   Confirming tags and fusion proteins. We’ll break down each of these steps...

  8. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...2: CLIP-SNAP fusion proteins for single-molecule FRET. A) Schematic of a CLIP-SNAP fusion protein (a.k.a...YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active (GTP-...

  9. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ...co-expressed with a fusion protein containing VP2C and a protein of interest (POI). The VP2C fusion protein anchors...while still allowing substrates and products to diffuse freely into or out of the protein compartment. ...

  10. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...Cas break for HR is to fuse an HR protein to the nuclease. Early HR factors fused to Cas9 have been shown..., A. H. Y., Menerot, S., Brion, A., et al. CtIP fusion to Cas9 enhances transgene integration by homology-dependent...

  11. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    ...live cells. Indeed, groups have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing...Leptotrichia wadei (LwaCas13a), using a superfolder GFP fusion to stabilize the protein in mammalian cells. LwaCas13a-msfGFP...programmable RNA-binding protein, creating the fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. ...

  12. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...integrase via protein fusions, using Prime to lay a “beacon” into the genome that a fused integrase would then... single protein, computationally described as a fusion of four Cas7 proteins and a putative Cas11-like...

  13. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ... Learn about in vivo biotinylation of bacterial fusion proteins Biotin is an essential vitamin for life...protein of interest with biotin by simply creating a fusion in which AviTag is added to either the N or C terminus...Addgene blog In vivo biotinylation of bacterial fusion proteins Learn how biotin is used in Split-BioID...

  14. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...colleagues devised a “Modular fluorescence unit fused with TRansmembrane region-to-IntrAcellular loop ...immunoreceptor tyrosinase-based activation motif (ITAM). By fusing ITAM to an RTK of interest and co-expressing a ...to meet those requirements (Lu et al. 2022). By fusing two bright fluorescent proteins with different ...

  15. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...tetracycline-controlled transactivator (tTA). They fused TetR with the transcriptional activation domain ... A chimeric transactivator, cTA, was created by fusing CymR to the activation domain VP16. cTA binds to...with LexA binding to the lexA operator (lexAop). Fusing the LexA DNA-binding domain (DBD) to GAL4 or VP16...

  16. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...are artificial restriction enzymes generated by fusing a zinc finger DNA-binding domain to a DNA-cleavage...to introduce point mutations in the genome. They fused a catalytically impaired Cas9 protein to a DNA-editing...in 2019. It’s based on a prime editor which is a fusion between Cas9 and reverse transcriptase. Using an...

  18. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...such as inducible expression of the hpRNA and/or fusing your gene target sequence to a fluorescent reporter...of the DNA construct, where your RNAi-target is fused to the DNA backbone that contains a target sequence...achieved this by creating constructs that directly fused any gene targeting sequence to a targeting sequence...

  19. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...as a fusion protein. An ScFv is made up of the variable regions of the heavy and light chains fused together...produced from hybridomas. Hybridomas are formed by fusing B cells that have been extracted from animals injected...

  20. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    .... Empty backbones often contain epitope tags or fusion proteins that are appended to new inserts through... the gene cloned into your plasmid, any tags or fusion proteins contained in the plasmid, any mutations...affinity for calcium". Please describe any tags or fusions (e.g. His, FLAG, EGFP, etc) on your insert. Use...
Showing: 101 - 120 of 282 results