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  1. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...environment.  Be kind to yourself. This is a challenging period for everyone for numerous reasons, and...

  3. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...cloned from include click beetles (Pyrophorus plagiophthalmus), railroad worm (Phrixothrix hirtus), and a... ATP No Jamaican click beetle(Pyrophorus plagiophthalamus) 544-593 64 Luciferin ATP No Railroad worms...

  4. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability...):337-47. PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries...

  5. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...

  6. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ...interested in studying genes whose knockout would be lethal to cells and thus not assayable with CRISPR. Another...could culture cells in the drug of interest at a lethal dose for non-manipulated cells and then assay cells...

  7. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...

  8. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...engineering designs and ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant...of students and teachers. It’s possible to join a half-day workshop for teachers, to schedule a three-day...

  10. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...the power 2-fold does not reduce photobleaching by half. How exactly the photobleaching rate depends on ...described by the t½, which is the time after which half of the initial fluorescence intensity remains. The...

  11. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...2015; Koike-Yusa et al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert... 26190106. PubMed Central PMCID: PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout...

  13. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...that can impact reproduction include embryonic lethality, a short lifespan, or infertility and can arise... number or expression level effects.  Another challenge that may arise when crossing mice with multiple...

  14. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    Published
    July 18, 2019, 12:55 p.m.
    ... tool by splitting cGAL4 in two and binding each half to a gp41-1-N-intein. Split inteins are protein ...With the split system, only cells that have both halves of cGAL can drive expression of your gene of interest...

  15. Hot Plasmids - October 2020

    Type
    Blog Post
    Published
    Oct. 13, 2020, 1:15 p.m.
    ...synaptic connections and physiology is particularly challenging as they are great in number, generally very small...H2B-FT-red is long lasting and was shown to have a half life of 84 hours in primary mouse embryonic fibroblasts...

  16. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...research project. In total, the interviews lasted from half to all of the day.  This whole process may seem ...carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably still...

  20. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...topic or that were published by the lab down the hall.  Scientific journals: They publish papers, so naturally...
Showing: 101 - 120 of 376 results