We narrowed to 388 results for: hal

...these indicators difficult to work with.  The Gottschalk lab has surveyed a range of microbial rhodopsin...previously reported for other organisms, and the Gottschalk group hypothesizes that this may be due to different...

... J, Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S (2006) Lethal Infection of...

...exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...environment.  Be kind to yourself. This is a challenging period for everyone for numerous reasons, and...

...Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands...could be played when and, depending upon who your challenger was, you might have to drastically alter your...

...seqWell, Inc., our mission is to overcome crucial challenges in NGS by developing technologies that can help...problem in multiplex NGS library preparation. The challenge here is that, as more samples are sequenced simultaneously...

...management, the journey can be as exciting as it is challenging. How do you prepare for this shift and navigate...experiences, and practical advice for overcoming challenges. Experimenting with leadership opportunities...

...interested in studying genes whose knockout would be lethal to cells and thus not assayable with CRISPR. Another...could culture cells in the drug of interest at a lethal dose for non-manipulated cells and then assay cells...

...such as sequencing or qPCR. Yay!  Where’s the challenge in that?  So while this method is certainly long...the sample type and size.) It can be quite the challenge, and take a few rounds of adjustments, to get ...

...Renilla reniformis), click beetle (Pyrophorus plagiophthalamus), deep-sea copepod (Gaussia princeps), Japanese...None No Click Beetle Red (CBR)(Pyrophorus plagiophthalamus) Natural or codon-optimized 613 (luciferin...

...engineering designs and ideas ranged from a biological breathalyzer to pesticide breakdown, production of fire-resistant...of students and teachers. It’s possible to join a half-day workshop for teachers, to schedule a three-day...

...makes generation of high titer Cas9 lentiviruses challenging. This cytotoxicity also causes growth rate variability...):337-47. PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries...

... (such as Nicotiana benthamiana or Arabidopsis thaliana (Xing et al., 2016)). During Agrobacterium-mediated...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...

...the power 2-fold does not reduce photobleaching by half. How exactly the photobleaching rate depends on ...described by the t½, which is the time after which half of the initial fluorescence intensity remains. The...

...2015; Koike-Yusa et al., 2014; Ma et al., 2015; Shalem et al., 2014; Wang et al., 2014), knock down (Gilbert... 26190106. PubMed Central PMCID: PMC4559080. 8. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9 knockout...

...that can impact reproduction include embryonic lethality, a short lifespan, or infertility and can arise... number or expression level effects.  Another challenge that may arise when crossing mice with multiple...

... tool by splitting cGAL4 in two and binding each half to a gp41-1-N-intein. Split inteins are protein ...With the split system, only cells that have both halves of cGAL can drive expression of your gene of interest...

...synaptic connections and physiology is particularly challenging as they are great in number, generally very small...H2B-FT-red is long lasting and was shown to have a half life of 84 hours in primary mouse embryonic fibroblasts...

...research project. In total, the interviews lasted from half to all of the day.  This whole process may seem ...carefully. And even if you don’t have technical challenges, your interviewers might.  You’ll probably still...

...Tebo et al. Nat Commun. 2019 Arnaud Gautier HaloTag TagBiFC allows exogenous labeling for single-molecule...and reversible complementation. TagBiFC (split HaloTag) provides maximum flexibility in color labeling...

...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...𝛽-globin), which cause sickle cell disease and 𝛽-thalassemia, can be mitigated with the expression of fetal...