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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 406 results for: mag

Showing: 101 - 120 of 406 results

  1. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    Published
    May 18, 2021, 1:15 p.m.
    ... antigens as measured by suspension bead assay. Image from  Preger et al., 2020. Preger et al., Journal...3: Schematic representation of the MPyV system. Image from Cheah et al., 2021.   After validating ... vectors expressing GCaMP6f with this promoter. Image calcium transients in PV interneurons with AAV-S5E2... terminal providing better signal to noise for imaging calcium dynamics in fine structures. We have expanded...

  2. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...engineering of biology much easier. So you could imagine engineering the entirety of a cell. That's a big... or so sensor domains for everything you could imagine. That I think is the next priority; we need the...clear, but the way I'd leave it is mostly to the imagination. The question is: what can you use biology to...off-the-shelf genetic elements that allow you to begin to imagine designing 10-gene systems with quantitative precision...

  3. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ... Math, sarcasm, romance, and spacecraft! Image courtesy of xkcd.com Science communication should...enough information to tell a compelling story in an image or do you need a full exhibit? In a five minute ... to convey an entire story or context via still images. Some things cannot be photographed or need text...interpretation. Infographics Low Combines both words and images; especially useful for conveying and contextualizing...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...mCyRFP1: Novel fluorescent protein that enables imaging of two biosensors simultaneously   Researchers...energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy) applications. To demonstrate the power...shown great performance in cellular functional imaging and in protein fusions. The Gadella lab has deposited...toolbox of fluorescent proteins (FP) for cellular imaging is constantly expanding. Case in point - check ...could be better suited for deep-tissue in vivo imaging. These variants were developed as part of a larger...

  5. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ...Cas9-mediated editing (ACE) reporter in action. Image from St. Martin et al., 2018. APOBEC-Cas9n-UGI...Editing efficiencies using base editing reporters. Image from St. Martin et al., 2019. The Harris lab ...results in shift in emission spectra from BFP to GFP. Image from Standage-Beier et al., 2019. The team put...luciferase reporter, and neomycin resistance reporter. Image adapted from Katti et al., 2020. As a proof of...

  6. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...temperature than its Spinach ancestors, and has a lower magnesium dependence. Though initially still unstable in...Central PMCID: PMC4364232. 2. Baker, Monya. “RNA imaging in situ”. Nature Methods 9, 787–790 (2012). 3. .... Filonov, Gregory S., et al. “In-gel imaging of RNA processing using broccoli reveals optimal aptamer...PMCID: PMC4071757. 6. Hong, He, et al. “Molecular Imaging with Nucleic Acid Aptamers.” Curr Med Chem.  2011...

  7. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    Published
    Oct. 27, 2015, 3 p.m.
    ...non-fluorescent, and misfolded FP-fusions on quantitative imaging experiments and cell physiology in general. Pitfalls...readily apparent and can confound quantitative imaging experiments or even negatively impact cells (see... represent one of the most inert solutions for imaging in a variety of cellular compartments including...proteins in the Endoplasmic Reticulum and live cell imaging approaches. Erik is also an avid long distance ...

  8. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...material is a factor of practicality (given image sizes) but images should be unprocessed and in capture format...other than the actual author. Instead, selected images are said to be representative or the statistical...charts and tables in the paper are in scope. For images, the number of examples supporting the published...

  9. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...blocking, and washing, the membrane is reassembled and imaged. In this example, a 1:1,000 dilution gives a high...eye; a fluorescent ladder compatible with your imaging system; or a combination of the two. There are ... wash for the full amount of time, every time. Imaging The last step in a western blot tends to be the...technical design of a western blot”) post, most imaging issues can be fixed real-time by exposing for either...seeing high background or low signal during the imaging process, try adjusting your exposure time first...

  10. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...Scientists around the world began looking for the magic molecule that serves as the code of life. In 1944...Raymond Gosling took the first ever X-ray diffracted image of the DNA. Finally, in 1953, Franklin along with...the two most popular topics that sparked the imagination of most of the authors during this period.  Genetic...engineering in pop culture SciFi is one of the most imaginative and most important genres of literary fiction...

  11. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

    Type
    Blog Post
    Published
    Nov. 6, 2018, 1:12 p.m.
    ...of regenerative medicine is to replace lost or damaged cells. Here, I will discuss the two major processes... is observed in the pancreas when excess β-cell damage results in the transdifferentiation of glucagon-producing...cells from the patient in vitro then correct the damaged gene in the resulting iPSCs before differentiating...to replenish and repair cells that are lost or damaged. Both transdifferentiation and reprogramming give...

  12. New Podcast Segment: Hot Plasmids

    Type
    Blog Post
    Published
    May 17, 2017, 3:37 p.m.
    ...Hot Plasmid 2: Magneto 2.0 form Ali Guler's Lab - Control neuronal activity with a magnetic field. Listen...Listen to Our Segment on Magneto 2.0 Hot Plasmid 3: The Human Kinase Domain Constructs Kit from John Chodera's...

  13. Activate Addgene's "Find Plasmids" Badge on Pubmed Abstracts

    Type
    Blog Post
    Published
    May 12, 2017, 12:58 p.m.
    ... beautiful Addgene “Find Plasmids” badge in the image below. If an abstract on PubMed has the “Find Plasmids... plasmids badge appear So how do you make this magical Addgene badge appear? First you’ll need to create...Preferences” and click on the “Filters & Icons” link (see image to the right). Once on the “Filters” page, select...

  14. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    Published
    Aug. 13, 2014, 3:49 p.m.
    ... them. For the application we were looking at, magnetic isolation of circulating tumor cells, we found...may have an impact on this challenge either for magnetic capture or for capture using microfluidic devices...peptide-peptide ligation polymerizes affibodies to enhance magnetic cancer cell capture. Fierer et al (Proc Natl Acad...

  15. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...retain the stop cassette following recombination. Image from Isaacman-Beck et al., 2019. Sparse Predictive...indicator, GCaMP6f, in the Drosophila optic lobe. Image from Isaacman-Beck et al., 2019. Applications...for optogenetics. The Clandinin lab used SPARC to image the calcium response in the dendrites of T5 neurons...

  16. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...control. C) Iterative transduction workflow overview. Image from Sun et al. 2021. The Organoid EasyTag ...of the receptor-based SL fluorescent biosensor. Image from Chesterfield et al., 2020     Chesterfield... calcium sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON vector 137128...

  17. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type
    Blog Post
    Published
    Dec. 13, 2017, 3:20 p.m.
    ...through entire papers, perhaps you search google images for specific experimental results but, obviously...simple Chrome plugin that allows you to save any image from articles openly available on PubMed Central.... For example, you might include a confirmatory image to show that a previously used reagent performs ...

  18. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...looking for songs and sounds to synchronize with the images thereby making the videos more real. To give you...box, the Big Bang Theory set, and then use our imaginations to mold it all into a full laboratory. It is...It was a great treat for us to put our rusty imaginations to use in this world where we are often trained...

  19. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    Published
    Sept. 26, 2017, 12:12 p.m.
    ... Pamela J. Hines PhD, Senior Editor at Science Magazine. Although we only walk one path at a time, the...spotted an opening for being an editor at Science magazine. That one I just could not resist applying for... Pamela J. Hines is a Senior Editor at Science magazine, published by the American Association for the...
Showing: 101 - 120 of 406 results