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We narrowed to 486 results for: mme

Showing: 101 - 120 of 486 results

  1. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...results to a standard curve. For CNV assays, it is recommended to use a reference sample with two copies of ...Taqman chemistry (see image, left, of droplet immersion technique, courtesy of CAR15.) Each droplet contains... 29717442. Arya M, Shergill IS, Williamson M, Gommersall L, Arya N, Patel HR. Basic principles of real-time...

  2. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    Published
    June 25, 2024, 1:15 p.m.
    ...trained our models in close collaboration with the Krauthammer lab (University of Zurich), especially with my...F., Schmidheini, L., Solari, C., Balázs, Z., Krauthammer, M., & Schwank, G. (2023). Predicting prime editing...Ioannidi, E. I., Böck, D., Steensel, B. van, Krauthammer, M., & Schwank, G. (2024). Machine learning prediction...

  3. Pooled Library Amplifications

    Type
    Blog Post
    Published
    May 30, 2024, 1:30 p.m.
    ...overexpression library) or is prone to recombination, we recommend amplification on solid media. If your plasmid ...recombination. If you do see recombination, we recommend ensuring the cells used are meant for repetitive...when developing your amplification protocol! We recommend looking at protocols used for libraries that are...

  4. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...Cell lysates are then treated with benzonase immediately before iodixanol purification. This approach ...production tips or tricks? Please share them in the comments below! Special thanks for Dr. Dan Stone and...AAV production and purification! References 1. Aurnhammer, C., Haase, M., Muether, N., Hausl, M.A., Rauschhuber...

  5. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...purification kits, it is low cost and can be immediately adopted by any laboratory. All procedures are...new DNA fragment, encode homology arms to allow immediate co-transformation with linearized vector backbone...This will minimize random errors during PCR: we recommend Phusion or Q5. Speed up your life Rather than ...

  6. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...beta-lactamase prior to division, instead of immediately experiencing the effects of the antibiotic. This...little more time, since bacteria won’t divide immediately after it has been heat-shocked or electrically...Resources References Hwang, J. M., Piccinini, T. E., Lammel, C. J., Hadley, W. K., & Brooks, G. F. (1986). ...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...but they cannot be entirely eliminated. It is recommended that you image samples before and after expansion...representation of reality. Third, expansion results in dimmer samples. Reduced signal can be caused by loss of...https://doi.org/10.1038/nbt.3625  Truckenbrodt S, Sommer C, Rizzoli SO, Danzl JG (2019) A practical guide...

  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    Published
    Aug. 13, 2024, 1:15 p.m.
    ...reliable expression, and easy distribution as DNA (Trimmer, 2020), you may be interested in using them in ... effective compared to those aliquots frozen immediately post-harvest. Stress-tested antibodies that are...Purification Protocol Addgene's Antibody Guide References Trimmer JS. Recombinant Antibodies in Basic Neuroscience...

  9. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...company. The Addgene summer picnic This past weekend was the annual Addgene Summer Picnic - a time when...sometimes teams disagree. Yes, sometimes work is a bummer when you’d rather be at the beach or binging a ...

  10. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...conjugated to agarose or magnetic beads with commercially available chemical agents. Directly conjugating...Protein A/G to immobilize the antibody, it is recommended to preclear the sample to remove any proteins...low pH buffer such as 0.1M glycine pH 2.5 and immediately neutralized with Tris pH 8-8.5. Low pH glycine...

  11. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...the difference between materials available from commercial repositories and the same cell line from a colleague...difference is the QC and validation carried out by the commercial repository. Repositories generally ensure cell...mutation rate of the cell line, but it is broadly recommended that the lower the passage number the better ...

  12. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ... the two fluorophore colours. Experts in FRET recommend to using two fluorophores that have a similar ...one that is better than another. It is often recommended by FRET experts to use as many measurement methods... Trends Biochem Sci. 2007 Sep; 32(9): 407-14. Zimmermann T,  Terjung S. FRET Basics and Applications: ...

  13. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...with almost 80% rating it as very important. One comment reflected the desire for a flexible generic solution...Important collaborative functionalities include comments, notifications, sharing files, assigning team ...actions. These records can take the form of written comments, added files, notes etc. Detailed records about...

  14. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

    Type
    Blog Post
    Published
    March 1, 2016, 3:30 p.m.
    ...DNA fragments. As a fun way to start, I highly recommend watching this entertaining video created by our...at 50 °C. After an hour or so, the sample is immediately ready to transform into competent cells. The ...contain the appropriate homologous sequences. NEB recommends an overlap of 15-40 bp, with a primer melting...

  15. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...crossed strand. While this pathway may not be immediately applicable in DSB-induced genome engineering,... the vector. Alternatively, PCR-generated or commercially synthesized linear dsDNA templates can be used...catalytically active and inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech....

  16. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...corresponding fluorophore-conjugated antibody commercially available. In other cases, fluorophore-conjugated...this system is that streptavidin conjugates are commercially available for a wide range of fluorophores, ...on the location of the targeted markers. Some commercially available fix/perm kits allow for labeling of...

  17. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...IPI’s anti-integrin rAbs are unconjugated and recommended for use in flow cytometry. Based on the established...resulting in cells that elongated and morphed asymmetrically (Jo et al., 2022). By contrast, LVDP-binding...conferred less force, leading cells to spread into symmetrical circles. While elucidating the role of tension...

  18. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...addition of translation-blocking STOP cassette immediately following the promoter. The STOP cassette includes...homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells. Snippert HJ, van der...Freundlich T, Weissman TA, Schoppik D, Wang XC, Zimmerman S, Ciruna B, Sanes JR, Luchtman JW, Schier AF....

  19. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...sections you’re particularly interested in, but I’d recommend watching the whole livestream for a more holistic...pre-publish) sound data even if it isn’t considered immediately impactful. Down the line we may see further adoption...issues in the biological sciences. I’d highly recommend listening to the panel discussion to learn a bit...

  20. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...template and CRISPR machinery, Quadros et al. recommend ribonucleoprotein (RNP) complexes of Cas9 protein...splice donor/acceptor sites, Miura et al. (2018) recommend targeting sites at least 100 bp away from the ...infundibulum. In vivo electroporation is performed immediately after injection. Image from Ohtsuka et al., 2018...
Showing: 101 - 120 of 486 results