...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...manager can be efficient without delegating and empowering the team to solve problems together (tactics ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...

...projects spanning from the identification of plant compounds and insecticidal proteins to the development of...Improvement of Cowpea, a knowledge database for this important agricultural plant. Her current research focuses... reside within living plants and that produce compounds with various antimicrobial activities (1). She... newly identified proteins and enzymes. These compounds will later get tested for their potential agricultural...

...worldwide and the presentation made it clear how important it is for us to improve our resources for this...Needless to say we all got very good at applying temporary tattoos on the spot with our trusty spray bottles...as well as the serious business of science. Two symposia on Sunday afternoon were dedicated to science ...Doench’s opening presentation at the CRISPR screens symposia outlined the fantastic work this Broad Institute...

...scientists start out in academia, it is especially important for graduate students and postdocs to get a perspective...interesting topics Having strong non-science skills is important for success Must always justify use of resources...rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting Work is...scientists following diverse career paths is an important first step. Developing mentoring relationships...

...downstream control – this is important later! Next, additional components are added - the ‘bells and whistles...times more flexible than the brain fibers and importantly distortion of the fibers didn’t affect their ...temperature proximal to the implanted fibers. As important as its capabilities are the practical considerations...

...for this purpose. This second area is extremely important and given the lack of attention to it, it is an...tool unique. The CRISPR Software Matchmaker is composed of these features and enables you to select the... interact with the software. This category is important for users wishing to select tools based on their...Matchmaker, it provides a list of terms I think are important to define for the CRISPR software space. It is...many different communities that it is all but impossible to fairly judge individual tools. I believe at...

...colors of orange and white. The decor was very contemporary and futuristic with block chairs in the hallway...must first fragment a DNA sample into many small components. Without proper processing, these small DNA strands...for Fragmentation System and this is the most important part of this newly released prep kit. In their...for the research community, so it is extremely important for us as a company to constantly update our processes...

...a PhD student in his first year. “It was very important to me to be with them in the lab when they started...the lab and to take things slowly! It is more important to carefully select first hires who are well-suited...people talk themselves out of great projects as ‘impossible' or useless.” How do you run your lab differently...a challenging process. Each PI emphasizes the importance of slowly building up your lab and selectively...

...cells. Before getting into the mammalian plasmid components, it may be useful to describe the means of introducing...void. These ORIs, however, require additional components expressed in trans within the cell for effective...ORIs, respectively. The presence of these viral components greatly reduces the rate of plasmid dilution ...Before doing a transfection experiment, it is important to determine the proper concentration required...

...transcription factor important for T cell differentiation (a cell type important for adaptive immune responses... to generate a transgenic fly There are a few important points to consider when choosing the vector you...affecting the body color of the adult fly). This is important to note so that you know what to look for. Making...

...about the process that was used to create this important collection.  Creating the Plasmids To begin constructing...antibody (R-mAb) expression plasmids, allowing for important reagents that could have been lost to continue...sequences available from each plasmid webpage. Importantly, the methods developed by the Trimmer Lab for...why choosing the right secondary is of primary importance. PLoS One. 2012;7(6):e38313. doi: 10.1371/journal.pone...

...nuclease for your experiments, there are three important factors to consider: site accessibility, specificity...potential for off-target cleavage should be an important consideration during any experiment. Sensitivity...stringent PAM specificity. This can be especially important for base editing techniques, where Cas9 must be...many options! Comic by Maya Kostman. Another important consideration when choosing a Cas9 is balancing...

... optogenetics is renowned for enabling precise temporal and spatial control. Optogenetics uses genetically... on and off gene expression while imparting spatiotemporal control, reversibility, and repeatability. .... The system developed by the Sata lab is composed of two fusion proteins: 1) the genomic anchor - an ...) A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR‐Cas9. Angew Chem Int Ed 58...Rauen RR, Kahn SI, Tsang M, Deiters A (2020) Spatiotemporal Control of CRISPR/Cas9 Function in Cells and...

...transcription factors with important roles in Multiple Myeloma), it is important to ensure lack of degradation...degradation In the Bradner and Gray labs, we synthesize compounds that enable selective removal of proteins-of-interest... chimeras For each protein-of-interest, it is important to determine which terminus can accommodate the...

...mutations. Moreover, introducing new DNA seems impossible with current tools, thus quieting some fears ...in cases of disease prevention. However, it is important to consider that already-tested CRISPR alternatives...clinical applications could be considered. It is important to consider the question of broader applicability... large step forward in the CRISPR field, it’s important to remember that the study hasn’t achieved any...

... how these structures are generated, why it’s important for cells to sense their formation, and how clinicians...in turn lead to genome instability. Thus, it’s important to strike a balance between productive levels ...be delivered or anticipating this response are important considerations when using these reagents. RNA...stranded isn’t just for DNA. Untwisting RNA is important for therapeutic delivery, viruses, human disease...

...Nature has evolved ways to prevent degradation of important cellular RNA molecules with post translational...Frontiers in Genome Editing). This is particularly important in experiments in which viability is being compromised...Ramel, MC., et al. Photoswitchable gRNAs for Spatiotemporally Controlled CRSIPR-Cas-based Genomic Regulation... CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...

... Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins of replication...recombination events. The system consists of two components derived from the P1 bacteriophage: the Cre recombinase...recognition site. The P1 bacteriophage uses these components as part of its natural viral life-cycle, and ...and researchers have adapted the components for use in genome manipulation. Cre recombinase, originally...

...project is the best approach to a given problem, Empowers the writer, And offers insight into a career ...understand the RFA. The RFA contains all of the important information required for a successful grant application... to determine the exact value of costs. It is important to be aware of supply costs per year, how many...Grant applications have a standard “Our work will empower the world” tone that differs from publication writing...

... grant early! First off, and perhaps the most important bit of advice I can suggest, is to give yourself...Originality is essential for grant success The two most important things reviewers will be looking for in your research...from accounts or HR on the judging panel. It’s important for this person to get a clear and concise explanation...going to be an element of luck involved so it’s important not to throw in the towel if 1 or 2 funding bodies...