We narrowed to 631 results for: rel

...efficiency of Cas9 cleavage is relatively high while the efficiency of HDR is relatively low, meaning that most...Anzalone et al., 2019). PE3, as originally introduced, relies on the PE2 enzyme. PE4 and PE5: Engineering mismatch...act first. PE4 and PE5, as originally introduced, rely on the PE2 enzyme. PEmax: Optimizing prime editor...

...widefield imaging fluorophores are excited with relatively low light levels (for a prolonged time). Other...illumination, TIRF or spinning disk confocal use entirely different excitation power regimes. Therefore,...experiments. As the photobleaching rate is not linearly related to power, the conclusions reached at high power...application (Goedhart et al, 2012). The third issue relates to the environment of the fluorescent protein. ...water/oil emulsion at high power, it is far more relevant to measure how fluorescence intensity evolves ...

...vector systems Gamma-retroviral vectors have a relatively high transduction efficiency, but in contrast...

...in the native virus. Since the WT sequence is relatively unstable, a more stable 130 bp version is used... D. V. (2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10,...

...binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins containing...or function of a single protein? Oftentimes, they rely on protein purification. In this strategy a single...contrast, the polyhistidine tag is very small and rarely affects protein function (Bornhorst 2000).  One...other affinity purification methods where binding relies on a specific protein conformation, the polyhistidine-metal...questions about polyhistidine tags. For more antibody-related information, sign up to receive alerts for antibody...

...our engineers (Fig. 2). Creating this guide was relatively easy. I took a picture of virtually every container...each given container, when applicable Any other relevant information Prescribing an official name to all...important documentation, including SOPs or project-related documents, we define new and potentially ambiguous...transferable skills Find all of our science career related blog posts Read our science communication blog ...

...DREADDs are ideal for chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...

...measurement. Like a p24 ELISA, this method is relatively quick, providing results in less than a day, ...

...structure, constructing split variants should be relatively straightforward, but it is also possible to create...

.... insulin). Proteins, unlike DNA which can be relatively easily synthesized, must be produced using complex...protein expression, S. cerevisiae, is the most reliable and frequently used species due to its use as ...

...If the phenotype is corrected, students can be relatively confident that the gene from the test species...

... are enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One ...These interactions are often modeled as binary relationships, i.e. protein A interacts with protein B; but...transient PPIs. Many PPI identification techniques rely on protein interactors to stay in close contact ...

...identical antibodies (See Fig 1.). rAbs are a relatively new technology that takes advantage of DNA sequencing...revealing the unreliability of antibodies. There are three main underlying causes of this unreliability: cross-reactivity...sifting through comments and papers to find the most reliable (and cost-effective) antibody for my experiment...the antibody can no longer bind to the target). Unreliable antibodies cause research that cannot be reproduced... of all commercially available antibodies are unreliable (Baker, 2015), causing a huge waste of precious...specific (minimal binding to off-target molecules), reliable (wouldn’t perform differently over time), compatible... thousands of scientists to confidently choose reliable and consistent antibodies for specific markers...

...support scientists wear many hats at this still relatively small nonprofit. If you do go longer than a few...these tips and tricks will apply to any biology-related customer support interaction. Before contacting...

...City The conference was located in the modern, relatively new “Science City” area on the outskirts of Guangzhouon...Rajewsky lab is studying circular RNAs (who knew…I had barely heard of these). These single stranded, covalently...of Georgia studies the CRISPR/Cas system as it relates to its natural role in prokaryotic immunity to ...

...genomes Compared to humans and mice, flies have a relatively simple genome. They have four chromosomes: the...females do not. Also, males have sex combs on their forelegs which are black, bristly hairs. Females do not...

...8.2 kb, and in vivo transgene expression ceases relatively quickly due to immune response against AdV. Importantly...

...While this method can be time-consuming, it is relatively effective in reducing off-target effects since...

...agarose gel to confirm product presence, size, and relative quantity. End point PCR is most commonly used ...reagents and several heating and cooling steps. PCR relies on heat resistant DNA polymerase from the thermophilic...

... 100-fold excess of wild-type bacteria and the relative frequency of each of the 40 library strains was...