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We narrowed to 628 results for: rel

Showing: 101 - 120 of 628 results

  1. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... generating iPSCs. The generation of iPSCs is relatively simple in concept: ectopically express a cocktail.... The advantages of this method are that it’s relatively simple to implement and doesn’t require time-...Overall, integrating viral vectors efficiently and reliably generate iPSCs, but they have several safety concerns...important control. Additionally, Sendai virus doesn’t rely on promoters for transcriptional regulation, so ...Check Out Our Interview with Stem Cell Researcher Darrell Kotton Four Factors that Differentiate the Stem...

  2. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...in the native virus. Since the WT sequence is relatively unstable, a more stable 130 bp version is used... D. V. (2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10,...

  3. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    Published
    June 8, 2017, 2:30 p.m.
    ...widefield imaging fluorophores are excited with relatively low light levels (for a prolonged time). Other...illumination, TIRF or spinning disk confocal use entirely different excitation power regimes. Therefore,...experiments. As the photobleaching rate is not linearly related to power, the conclusions reached at high power...application (Goedhart et al, 2012). The third issue relates to the environment of the fluorescent protein. ...water/oil emulsion at high power, it is far more relevant to measure how fluorescence intensity evolves ...

  5. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins containing...or function of a single protein? Oftentimes, they rely on protein purification. In this strategy a single...contrast, the polyhistidine tag is very small and rarely affects protein function (Bornhorst 2000).  One...other affinity purification methods where binding relies on a specific protein conformation, the polyhistidine-metal...questions about polyhistidine tags. For more antibody-related information, sign up to receive alerts for antibody...

  6. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    Published
    Nov. 21, 2019, 1:53 p.m.
    ...our engineers (Fig. 2). Creating this guide was relatively easy. I took a picture of virtually every container...each given container, when applicable Any other relevant information Prescribing an official name to all...important documentation, including SOPs or project-related documents, we define new and potentially ambiguous...transferable skills Find all of our science career related blog posts Read our science communication blog ...

  7. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...DREADDs are ideal for chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...

  8. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...measurement. Like a p24 ELISA, this method is relatively quick, providing results in less than a day, ...

  10. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    .... insulin). Proteins, unlike DNA which can be relatively easily synthesized, must be produced using complex...protein expression, S. cerevisiae, is the most reliable and frequently used species due to its use as ...

  12. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ... are enriched relative to controls using Split-BioID-GFP and BioID run on six unrelated proteins. One ...These interactions are often modeled as binary relationships, i.e. protein A interacts with protein B; but...transient PPIs. Many PPI identification techniques rely on protein interactors to stay in close contact ...

  13. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...identical antibodies (See Fig 1.). rAbs are a relatively new technology that takes advantage of DNA sequencing...revealing the unreliability of antibodies. There are three main underlying causes of this unreliability: cross-reactivity...sifting through comments and papers to find the most reliable (and cost-effective) antibody for my experiment...the antibody can no longer bind to the target). Unreliable antibodies cause research that cannot be reproduced... of all commercially available antibodies are unreliable (Baker, 2015), causing a huge waste of precious...specific (minimal binding to off-target molecules), reliable (wouldn’t perform differently over time), compatible... thousands of scientists to confidently choose reliable and consistent antibodies for specific markers...

  14. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...support scientists wear many hats at this still relatively small nonprofit. If you do go longer than a few...these tips and tricks will apply to any biology-related customer support interaction. Before contacting...

  15. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    Published
    Dec. 1, 2015, 3:30 p.m.
    ...City The conference was located in the modern, relatively new “Science City” area on the outskirts of Guangzhouon...Rajewsky lab is studying circular RNAs (who knew…I had barely heard of these). These single stranded, covalently...of Georgia studies the CRISPR/Cas system as it relates to its natural role in prokaryotic immunity to ...

  19. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...attending the session, and what is their background relative to your research? Tailor your language to your... you may receive. People often ask about the relationship of your work to recently published papers or...audience just that. Explain what you do know about a related topic, and let them know while you appreciate the...collaborate with a group who presented on a topic related to your research? Find a way to get in contact ...
Showing: 101 - 120 of 628 results