We narrowed to 139 results for: tox

...performed 12–18 hours post-transfection to help remove toxic transfection reagents and keep the cells happy. ...

...Infects dividing and non-dividing cells. AAV is least toxic method for in vivo viral delivery. Cas9 mRNA and...

...order to improve axial resolution and reduce phototoxicity for imaging live specimens, they combined light...

...the Brunello knock-out library while mitigating toxicity induced by high copy number dsDNA cutting. A Dolcetto...

...long-term expression; and 3) they have limited toxicity. However, a key challenge of using AAVs for neuroscience...

...pair change in DNA gyrase, CRISPR phagemids were cytotoxic only for resistant bacteria. Bikard et al. used...

...fluorescent dyes of the time was the fact that it was non-toxic and could be expressed in living cells, enabling...

...the Survival of MeCP2-Null Mice without Apparent Toxicity after Intracisternal Delivery. Molecular Therapy...

...did so without a substantial immune response or toxicity. More AAV-based CRISPR systems Smaller Cas proteins...

...to spontaneously develop resistance to selection toxins. Auxotrophic selection is useful for producing ...

...visualization technique. Ultimately, MG proved to be too toxic for many in vivo applications, but this work proved...

...containing the ccdB gene. Since the ccdB product is toxic to the cell, Gateway cloning efficiency can reach...

...career topics. However, many of the posts were about toxic advisors, fruitless job searches, and even despair...

... protein, which can be useful if the product is toxic in high amounts. These plasmids are also useful ...

...even kill your cells if your gene of interest is toxic. To combat this, scientists have created synthetic...

...for the sake of disagreeing. It can be extremely toxic in a lab or work environment if left unchecked. ...

...non-functional, they could be dominant-negative and/or toxic. A potential solution is to include an in-frame ...

...specificity, so in some cases they can lead to toxicity and off target effects. More recently, shRNA plasmids...

...Cas9 is no longer necessary. However, Cas9 can be toxic if continuously expressed (Fu et al., 2013). Using...

...methyltransferase for epigenetic silencing, which can be toxic, CHARM uses a cofactor fused to a histone tail to...