We narrowed to 855 results for: NTS

...such as RNaseZAP. When harvesting tissues, cells, plants, fungi, or bacteria, keep samples cold and work...the homogenate was loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate...instead of RNA separation using cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform...

...restriction sites to easily assemble multiple DNA fragments. This system has been used to construct plasmids...McCormick lab generated knock-out and knock-in mutants, determined protein expression levels from different...critical for reducing Cre toxicity in long-term experiments.  Figure 4: The C-terminus of Cre (CreC...

...reactions on tissues can be cumbersome Tyramide reagents can be expensive Can only stain with one HRP-RANbody...consider leaving a link to its sequence in the comments below. References Sanes, J.R., & Yamagata, M....versatile, small, sensitive immunohistochemical reagents. PNAS. PubMed PMID: 29440485. Additional Resources...

...about it. Building a diverse team with different talents and expertise Fortunately, the eLife ambassadors... had previous experience organizing open access events and grassroots efforts. After thoughtful conversations...Twitter (@repro4everyone), or email the team. Acknowledgements One of the highlights of 2018 for me was to...

...It’s also a great way to keep track of past accomplishments. Follow all the rules of good resume writing...writing. Include examples of concrete accomplishments in both the technical and “plus skills” areas. Include...’s news and updates. Share your own good ideas, events and favorite resources. Respect the network and...

... 4B and C in Zhou et al for details on these experiments. Photoswitchable kinases could possibly be used...can I try using photoswitchable kinases in my experiments? You can find the plasmids from Zhou et al here...Carrasco, and Markus W. Covert. "High-Sensitivity Measurements of Multiple Kinase Activities in Live Single...

... aggregate. The basics and pitfalls of these experiments are discussed here. In vitro tests for aggregation...your cell type of interest before starting your experiments.  Selecting a non-oligomerizing protein A fluorescent... Of course, you want the ‘latest and greatest’ variants with the best properties for your research. But...

...understand mechanisms and, eventually, develop treatments, we need tools to specifically edit methylation...original methylated strand is diluted to undetectable amounts: ergo, DNA demethylation! With optimization, this...Genome-wide tracking of dCas9-methyltransferase footprints. Nat Commun 9, 597. https://doi.org/10.1038/s41467...

...result to a reference sequence to confirm backbone elements.   Confirming the gene/insert by aligning to NCBI...origin of replication or other common backbone elements. Since we’ve successfully grown the plasmid in... about our sequencing results in the Depositor Comments section at the bottom of the plasmid page. Viral...

...of the mutations engineered into each of their parents. Here’s how to cross mice, using a simple example... all of your desired alleles to conduct your experiments. You should also maintain your parental strains...contain a mix of genetic material from each of the parents. If both of the mutations that you are trying to...